Strain identifier

BacDive ID: 1355

Type strain: Yes

Species: Halobacillus alkaliphilus

Strain Designation: FP5

Strain history: <- B. Nicolaus <- I. Romano; FP5

NCBI tax ID(s): 396056 (species)

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General

@ref: 7576

BacDive-ID: 1355

DSM-Number: 18525

keywords: genome sequence, 16S sequence, Bacteria, aerobe, halophilic, spore-forming, mesophilic, Gram-positive, coccus-shaped, colony-forming

description: Halobacillus alkaliphilus FP5 is an aerobe, halophilic, spore-forming bacterium that forms circular colonies and was isolated from salt lake.

NCBI tax id

  • NCBI tax id: 396056
  • Matching level: species

strain history

  • @ref: 7576
  • history: <- B. Nicolaus <- I. Romano; FP5

doi: 10.13145/bacdive1355.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Halobacillus
  • species: Halobacillus alkaliphilus
  • full scientific name: Halobacillus alkaliphilus Romano et al. 2008

@ref: 7576

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Halobacillus

species: Halobacillus alkaliphilus

full scientific name: Halobacillus alkaliphilus Romano et al. 2008

strain designation: FP5

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell shapemotilityconfidence
22992positive1.57 µmcoccus-shapedno
69480yes94.492
69480positive100

colony morphology

  • @ref: 22992
  • colony size: 1.0-2.0 mm
  • colony color: pale orange
  • colony shape: circular
  • medium used: agar medium

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7576HALOBACILLUS MEDIUM (DSMZ Medium 755)yeshttps://mediadive.dsmz.de/medium/755Name: HALOBACILLUS MEDIUM (DSMZ Medium 755) Composition: NaCl 100.0 g/l Peptone 5.0 g/l MgSO4 x 7 H2O 5.0 g/l Yeast extract 3.0 g/l Distilled water
22992agar mediumyes

culture temp

@refgrowthtypetemperaturerange
7576positivegrowth30mesophilic
22992positivegrowth25.0-45.0
22992maximum50.0thermophilic
22992positiveoptimum37.0mesophilic

culture pH

@refabilitytypepHPH range
22992positivegrowth6.0-10.0alkaliphile
22992positiveoptimum9.0

Physiology and metabolism

oxygen tolerance

  • @ref: 22992
  • oxygen tolerance: aerobe

spore formation

@refspore descriptionspore formationconfidence
22992sphericalyes
69481yes100
69480yes99.543

halophily

@refhalophily levelsaltgrowthtested relationconcentration
22992halophilicNaClpositivegrowth0.5-20.0 %(w/v)
22992NaCloptimum10.0 %(w/v)

murein

@refmurein short keytype
7576A21.04A4ß L-Orn-D-Asp
22992A21.04A4ß L-Orn-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2299217057cellobiose-builds acid from
2299212936D-galactose-builds acid from
22992casein-hydrolysis
229925291gelatin-hydrolysis
22992606565hippurate-hydrolysis
2299228017starch-hydrolysis
2299217632nitrate-reduction
2299228757fructose+builds acid from
2299217234glucose+builds acid from
2299217306maltose+builds acid from
2299237684mannose+builds acid from
2299216634raffinose+builds acid from
2299233942ribose+builds acid from
2299217992sucrose+builds acid from
2299227082trehalose+builds acid from
2299218222xylose+builds acid from
2299218186tyrosine+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
229927660nystatinyesyes100 µg (disc)
2299217076streptomycinyesyes25 µg (disc)
229926104kanamycinyesyes30 µg (disc)
229927507neomycinyesyes30 µg (disc)
2299217334penicillinyesyes10 Unit
2299228669bacitracinyesyes10 µg (disc)
2299217698chloramphenicolyesyes10 µg (disc)
2299271321fusidateyesyes10 µg (disc)
229926472lincomycinyesyes10 µg (disc)
2299228971ampicillinyesyes25 µg (disc)
2299228368novobiocinyesyes30 µg (disc)
2299227902tetracyclineyesyes30 µg (disc)
2299228001vancomycinyesyes30 µg (disc)
2299248923erythromycinyesyes5 µg (disc)

enzymes

@refvalueactivityec
22992catalase+1.11.1.6
22992cytochrome oxidase+1.9.3.1
22992phenylalanine deaminase-4.3.1.5
22992urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
22992salt lakeFuente de PiedraSpainESPEurope37.1-4.73333
7576salt lakeFuente de PiedraSpainESPEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_3747.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_469;97_536;98_617;99_3747&stattab=map
  • Last taxonomy: Halobacillus
  • 16S sequence: AM295006
  • Sequence Identity:
  • Total samples: 448
  • soil counts: 116
  • aquatic counts: 246
  • animal counts: 13
  • plant counts: 73

Safety information

risk assessment

  • @ref: 7576
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7576
  • description: Halobacillus alkaliphilus 16S rRNA gene, type strain FP5T
  • accession: AM295006
  • length: 1484
  • database: ena
  • NCBI tax ID: 396056

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Halobacillus alkaliphilus FP5GCA_900113125scaffoldncbi396056
66792Halobacillus alkaliphilus FP52654587900draftimg396056

GC content

@refGC-contentmethod
757643.5
2299243.5high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes86.289no
flagellatedyes78.435yes
gram-positiveyes90.692yes
anaerobicno98.903yes
aerobicyes95.754yes
halophileyes95.273no
spore-formingyes91.925no
glucose-fermentno89.34no
thermophileno98.624no
glucose-utilyes90.862no

External links

@ref: 7576

culture collection no.: DSM 18525, ATCC BAA 1361

straininfo link

  • @ref: 71006
  • straininfo: 298318

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18398188Halobacillus alkaliphilus sp. nov., a halophilic bacterium isolated from a salt lake in Fuente de Piedra, southern Spain.Romano I, Finore I, Nicolaus G, Huertas FJ, Lama L, Nicolaus B, Poli AInt J Syst Evol Microbiol10.1099/ijs.0.65457-02008Bacillaceae/*classification/genetics/*isolation & purification/metabolism, Base Composition, Base Sequence, DNA Primers/genetics, DNA, Bacterial/chemistry/genetics, Fatty Acids/metabolism, Fresh Water/microbiology, Genes, Bacterial, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Salinity, Spain, Terminology as TopicMetabolism
Phylogeny25795064Halobacillus andaensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soil.Wang K, Zhang L, Yang Y, Pan Y, Meng L, Liu H, Hong S, Huang H, Jiang JInt J Syst Evol Microbiol10.1099/ijs.0.0001982015Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Halobacillus/*classification/genetics/isolation & purification, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Soil/chemistry, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7576Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18525)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18525
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
22992Ida Romano,Ilaria Finore,Giancarlo Nicolaus,F. Javier Huertas,Licia Lama,Barbara Nicolaus,Annarita Poli10.1099/ijs.0.65457-0Halobacillus alkaliphilus sp. nov., a halophilic bacterium isolated from a salt lake in Fuente de Piedra, southern SpainIJSEM 58: 886-890 200818398188
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71006Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID298318.1StrainInfo: A central database for resolving microbial strain identifiers