Strain identifier

BacDive ID: 135388

Type strain: No

Species: Ralstonia insidiosa

Strain history: CIP <- 1994, Baxter, Roissy, France, Pseudomonas pickettii <- ATCC <- G.J. De Leon: strain AmMS 155, Pseudomonas cepacia

NCBI tax ID(s): 329 (species)

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General

@ref: 34494

BacDive-ID: 135388

keywords: genome sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Ralstonia insidiosa CIP 104062 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from Human.

NCBI tax id

  • NCBI tax id: 329
  • Matching level: species

strain history

  • @ref: 34494
  • history: CIP <- 1994, Baxter, Roissy, France, Pseudomonas pickettii <- ATCC <- G.J. De Leon: strain AmMS 155, Pseudomonas cepacia

doi: 10.13145/bacdive135388.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Ralstonia
  • species: Ralstonia insidiosa
  • full scientific name: Ralstonia insidiosa Coenye et al. 2003

@ref: 34494

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Ralstonia

species: Ralstonia insidiosa

type strain: no

Morphology

cell morphology

  • @ref: 34494
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 34494
  • production: yes
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
34494MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
34494CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
34494positivegrowth30mesophilic
34494nogrowth5psychrophilic
34494nogrowth41thermophilic
34494nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 34494
  • oxygen tolerance: obligate aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3449417632nitrate-reduction
3449416301nitrite-reduction
3449415882phenol-degradation
3449417632nitrate-respiration

antibiotic resistance

  • @ref: 34494
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 34494
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
34494oxidase+
34494beta-galactosidase-3.2.1.23
34494alcohol dehydrogenase-1.1.1.1
34494gelatinase-
34494amylase-
34494DNase-
34494caseinase-3.4.21.50
34494catalase-1.11.1.6
34494tween esterase-
34494lecithinase-
34494lipase-
34494lysine decarboxylase-4.1.1.18
34494ornithine decarboxylase-4.1.1.17
34494tryptophan deaminase-
34494urease-3.5.1.5

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
34494+++----------------------------------------+---+-+++-++-----+-+++--------+-++--++-+-----++++++-++++

Isolation, sampling and environmental information

isolation

  • @ref: 34494
  • sample type: Human

Safety information

risk assessment

  • @ref: 34494
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Ralstonia insidiosa ATCC 49129GCA_001663855completencbi190721
66792Ralstonia insidiosa strain ATCC 49129190721.12plasmidpatric190721
66792Ralstonia insidiosa strain ATCC 49129190721.7completepatric190721
66792Ralstonia insidiosa strain ATCC 49129190721.13plasmidpatric190721
66792Ralstonia insidiosa ATCC 491292721755751completeimg190721

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedyes61.472no
gram-positiveno98.575no
anaerobicno98.963no
aerobicyes91.463no
halophileno93.571no
spore-formingno93.899no
glucose-utilyes79.106no
motileyes89.189no
thermophileno99.411no
glucose-fermentno90.62no

External links

@ref: 34494

culture collection no.: CIP 104062, ATCC 49129

straininfo link

  • @ref: 92755
  • straininfo: 10815

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
34494Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104062Collection of Institut Pasteur (CIP 104062)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
92755Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID10815.1