Strain identifier

BacDive ID: 1353

Type strain: Yes

Species: Halobacillus mangrovi

Strain Designation: MS10

Strain history: <- CCM <- A. Ventosa; MS10

NCBI tax ID(s): 402384 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 15621

BacDive-ID: 1353

DSM-Number: 21335

keywords: 16S sequence, Bacteria, obligate aerobe, moderately halophilic, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Halobacillus mangrovi MS10 is an obligate aerobe, moderately halophilic, spore-forming bacterium that forms circular colonies and was isolated from leave surface, black mangrove.

NCBI tax id

  • NCBI tax id: 402384
  • Matching level: species

strain history

  • @ref: 15621
  • history: <- CCM <- A. Ventosa; MS10

doi: 10.13145/bacdive1353.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Halobacillus
  • species: Halobacillus mangrovi
  • full scientific name: Halobacillus mangrovi Soto-Ramírez et al. 2008

@ref: 15621

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Halobacillus

species: Halobacillus mangrovi

full scientific name: Halobacillus mangrovi Soto-Ramírez et al. 2008

strain designation: MS10

type strain: yes

Morphology

cell morphology

  • @ref: 22990
  • gram stain: positive
  • cell length: 1.0-2.0 µm
  • cell width: 0.5-1.0 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 22990
  • colony color: cream-brown
  • colony shape: circular

pigmentation

  • @ref: 22990
  • production: yes
  • color: cream

Culture and growth conditions

culture medium

  • @ref: 15621
  • name: MODIFIED BACTO MARINE BROTH (DSMZ Medium 514d)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/514d
  • composition: Name: MODIFIED BACTO MARINE BROTH (DSMZ Medium 514d) Composition: Difco marine broth 37.4 g/l Agar 15.0 g/l Malt extract 1.0 g/l Soy peptone 1.0 g/l Pancreatic digest of casein 1.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
15621positivegrowth35mesophilic
22990positivegrowth10.0-50.0
22990positiveoptimum33.0-35.0mesophilic

culture pH

@refabilitytypepHPH range
22990positivegrowth6.0-9.0alkaliphile
22990positiveoptimum7.0

Physiology and metabolism

oxygen tolerance

  • @ref: 22990
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 22990
  • spore formation: yes

halophily

@refhalophily levelsaltgrowthtested relationconcentration
22990moderately halophilicNaClpositivegrowth5.0-20.0 %(w/v)
22990NaCloptimum10.0 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
22990174265-dehydro-D-gluconate-builds acid from
2299015963ribitol-builds acid from
2299017057cellobiose-builds acid from
2299018333D-arabitol-builds acid from
2299012936D-galactose-builds acid from
2299018024D-galacturonic acid-builds acid from
2299028053melibiose-builds acid from
2299017924D-sorbitol-builds acid from
2299028757fructose-builds acid from
2299017234glucose-builds acid from
2299017268myo-inositol-builds acid from
2299030849L-arabinose-builds acid from
2299018403L-arabitol-builds acid from
2299062345L-rhamnose-builds acid from
2299017306maltose-builds acid from
2299029864mannitol-builds acid from
2299037684mannose-builds acid from
2299018394palatinose-builds acid from
2299030911sorbitol-builds acid from
2299017992sucrose-builds acid from
2299027082trehalose-builds acid from
2299018222xylose-builds acid from
2299017234glucose-fermentation
229904853esculin-hydrolysis
2299017632nitrate-reduction
2299017634D-glucose+carbon source
2299017924D-sorbitol+carbon source
2299028017starch+carbon source
2299030031succinate+carbon source
2299017634D-glucose+energy source
2299017924D-sorbitol+energy source
2299028017starch+energy source
2299030031succinate+energy source
22990casein+hydrolysis
229905291gelatin+hydrolysis
2299028017starch+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is intermediateintermediate conc.is sensitivesensitivity conc.
229908309polymyxin byesyes300 Unit
2299017076streptomycinyesyes10 µg (disc)
229907507neomycinyesyes30 µg (disc)
2299048923erythromycinyesyes15 µg (disc)
22990100147nalidixic acidyesyes30 µg (disc)
2299027902tetracyclineyesyes30 µg (disc)
2299028669bacitracinyesyes10 Unit
2299017334penicillinyesyes10 Unit
229903542cephalothinyesyes30 µg (disc)
2299017698chloramphenicolyesyes30 µg (disc)
229906104kanamycinyesyes30 µg (disc)
2299028368novobiocinyesyes30 µg (disc)
2299028001vancomycinyesyes30 µg (disc)
2299028077rifampicinyesyes5 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
2299035581indoleno
2299016136hydrogen sulfideno
229904853esculinno
2299015688acetoinno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
2299015688acetoin-
2299017234glucose-

enzymes

@refvalueactivityec
22990alpha-galactosidase+3.2.1.22
22990alpha-glucosidase+3.2.1.20
22990alpha-mannosidase+3.2.1.24
22990arginine dihydrolase-3.5.3.6
22990beta-galactosidase+3.2.1.23
22990beta-glucosidase-3.2.1.21
22990beta-glucuronidase-3.2.1.31
22990catalase+1.11.1.6
22990cytochrome oxidase+1.9.3.1
22990L-aspartate arylamidase-3.4.11.21
22990lipase (C 14)-
22990lysine decarboxylase-4.1.1.18
22990N-acetyl-beta-glucosaminidase-3.2.1.52
22990ornithine decarboxylase-4.1.1.17
22990urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
15621leave surface, black mangrovePuerto Rico, Cabo RojoPortugalPRTEurope
22990agar plates containing Sehgal-Gibbons mediumSG; 15 % NaCl, 2 % MgSO 4 . 7H 2 O, 0.2 % KCl, 0.3 % trisodium citrate, 0.002 % FeCl 2 , 0.75 % Casamino acids, 1 % yeast extract (w/v), pH 7.5-7.83 days30.0

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Tree
#Host Body-Site#Plant#Leaf (Phyllosphere)

Safety information

risk assessment

  • @ref: 15621
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15621
  • description: Halobacillus mangrovi strain MS10 16S ribosomal RNA gene, partial sequence
  • accession: DQ888316
  • length: 1460
  • database: ena
  • NCBI tax ID: 402384

GC content

@refGC-contentmethod
1562145.7
2299045.7thermal denaturation, midpoint method (Tm)

External links

@ref: 15621

culture collection no.: DSM 21335, CCM 7397, CECT 7206

straininfo link

  • @ref: 71004
  • straininfo: 323970

literature

  • topic: Phylogeny
  • Pubmed-ID: 18175696
  • title: Halobacillus mangrovi sp. nov., a moderately halophilic bacterium isolated from the black mangrove Avicennia germinans.
  • authors: Soto-Ramirez N, Sanchez-Porro C, Rosas-Padilla S, Almodovar K, Jimenez G, Machado-Rodriguez M, Zapata M, Ventosa A, Montalvo-Rodriguez R
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.65008-0
  • year: 2008
  • mesh: Avicennia/*microbiology, Bacillaceae/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, Fatty Acids/analysis, Genes, rRNA, Genotype, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Plant Leaves/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium Chloride, Species Specificity
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15621Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21335)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21335
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
22990Nelís Soto-Ramírez,Cristina Sánchez-Porro,Soniris Rosas-Padilla,Karinna Almodóvar,Gina Jimínez,Marlene Machado-Rodríguez,Magaly Zapata,Antonio Ventosa,Rafael Montalvo-Rodríguez10.1099/ijs.0.65008-0Halobacillus mangrovi sp. nov., a moderately halophilic bacterium isolated from the black mangrove Avicennia germinansIJSEM 58: 125-130 200818175696
71004Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID323970.1StrainInfo: A central database for resolving microbial strain identifiers