Strain identifier

BacDive ID: 135270

Type strain: No

Species: Bacillus subtilis

Strain Designation: FE 9

Strain history: CIP <- 1976, G. Balassa: strain FE 9

NCBI tax ID(s): 1423 (species)

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General

@ref: 34335

BacDive-ID: 135270

keywords: Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Bacillus subtilis FE 9 is an obligate aerobe, spore-forming, mesophilic bacterium of the family Bacillaceae.

NCBI tax id

  • NCBI tax id: 1423
  • Matching level: species

strain history

@refhistory
343351976, G. Balassa: strain FE 9
34335CIP <- 1976, G. Balassa: strain FE 9

doi: 10.13145/bacdive135270.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus subtilis
  • full scientific name: Bacillus subtilis (Ehrenberg 1835) Cohn 1872 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Vibrio subtilis

@ref: 34335

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Bacillus

species: Bacillus subtilis

strain designation: FE 9

type strain: no

Morphology

cell morphology

  • @ref: 34335
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 34335

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
34335MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
34335CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
34335positivegrowth30
34335positivegrowth22-55
34335nogrowth10

culture pH

  • @ref: 34335
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 34335
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 34335
  • spore formation: yes

halophily

  • @ref: 34335
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3433516947citrate+carbon source
343354853esculin+hydrolysis
34335606565hippurate+hydrolysis
3433517632nitrate+reduction
3433516301nitrite-reduction
3433517632nitrate-respiration
68371Potassium 5-ketogluconate-builds acid from
6837127613amygdalin+builds acid from
68371320061methyl alpha-D-glucopyranoside+builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate+builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837159640N-acetylglucosamine+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol+builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol+builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837130849L-arabinose+builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from

metabolite production

  • @ref: 34335
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
3433515688acetoin+
3433517234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382alpha-glucosidase+3.2.1.20
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin-3.4.21.1
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
34335oxidase-
34335beta-galactosidase+3.2.1.23
34335alcohol dehydrogenase-1.1.1.1
34335gelatinase+
34335amylase+
34335DNase+
34335caseinase+3.4.21.50
34335catalase+1.11.1.6
34335tween esterase+
34335gamma-glutamyltransferase+2.3.2.2
34335lecithinase-
34335lipase-
34335lysine decarboxylase-4.1.1.18
34335ornithine decarboxylase-4.1.1.17
34335protease+
34335urease+3.5.1.5
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382beta-glucuronidase-3.2.1.31

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
34335-+++------++---++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
34335+--++/-+/-----+++---+++-+++++++---++---+/-+-++------+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
34335++-++--+-------++++++++--------++++-+----+-++----+++-+-+---++------------+-+---++-----+--+++-+-----

Safety information

risk assessment

  • @ref: 34335
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 34335

culture collection no.: CIP 76.30

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
34335Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2076.30Collection of Institut Pasteur (CIP 76.30)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym