Strain identifier

BacDive ID: 134232

Type strain: Yes

Species: Azospirillum canadense

Strain Designation: DS2

Strain history: CIP <- 2007, G. Lazarovits, Agri Food Canada, London, Ontario, Canada: strain DS2

NCBI tax ID(s): 403962 (species)

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General

@ref: 32008

BacDive-ID: 134232

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, Gram-negative, motile, rod-shaped

description: Azospirillum canadense DS2 is an obligate aerobe, Gram-negative, motile bacterium that was isolated from Environment, Corn rhizosphere.

NCBI tax id

  • NCBI tax id: 403962
  • Matching level: species

strain history

  • @ref: 117123
  • history: CIP <- 2007, G. Lazarovits, Agri Food Canada, London, Ontario, Canada: strain DS2

doi: 10.13145/bacdive134232.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Azospirillaceae
  • genus: Azospirillum
  • species: Azospirillum canadense
  • full scientific name: Azospirillum canadense Mehnaz et al. 2007

@ref: 32008

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodospirillales

family: Rhodospirillaceae

genus: Azospirillum

species: Azospirillum canadense

strain designation: DS2

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
32008negative2.15 µm0.9 µmrod-shapedyes
117123negativerod-shapedyes

Culture and growth conditions

culture medium

@refnamegrowthlink
37813MEDIUM 697 -for Azospirillum canadenseyes
117123CIP Medium 321yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=321

culture temp

@refgrowthtypetemperaturerange
32008positivegrowth20-37
32008positiveoptimum27.5mesophilic
37813positivegrowth30mesophilic

culture pH

@refabilitytypepH
32008positivegrowth05-07
32008positiveoptimum6

Physiology and metabolism

oxygen tolerance

  • @ref: 117123
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
32008NaClpositivegrowth0.5-1.0 %
32008NaClpositiveoptimum0.75 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3200830089acetate+carbon source
3200816449alanine+carbon source
3200822653asparagine+carbon source
3200835391aspartate+carbon source
3200816947citrate+carbon source
3200815740formate+carbon source
3200824265gluconate+carbon source
3200824996lactate+carbon source
3200825115malate+carbon source
3200815792malonate+carbon source
3200817272propionate+carbon source
3200851850methyl pyruvate+carbon source
3200826490quinate+carbon source
3200830031succinate+carbon source
3200817632nitrate+reduction
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
11712317632nitrate+reduction
11712316301nitrite-reduction

metabolite production

  • @ref: 117123
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
32008catalase+1.11.1.6
32008cytochrome oxidase+1.9.3.1
117123oxidase+
117123catalase+1.11.1.6
117123urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117123--+--++---+---------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
117123------------------------+/-------------------------

Isolation, sampling and environmental information

isolation

  • @ref: 117123
  • sample type: Environment, Corn rhizosphere
  • geographic location: Delhi, Ontario
  • country: Canada
  • origin.country: CAN
  • continent: North America

Safety information

risk assessment

  • @ref: 117123
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 32008
  • description: Azospirillum canadense strain DS2 16S ribosomal RNA gene, partial sequence
  • accession: DQ393891
  • length: 1481
  • database: nuccore
  • NCBI tax ID: 403962

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Azospirillum canadense DS22928826531draftimg191
66792Azospirillum canadense DS2GCA_025961225contigncbi403962

GC content

  • @ref: 32008
  • GC-content: 67.9

External links

@ref: 32008

culture collection no.: NCCB 100108, LMG 23617, CIP 109702

straininfo link

  • @ref: 91826
  • straininfo: 297130

literature

  • topic: Phylogeny
  • Pubmed-ID: 17329796
  • title: Azospirillum canadense sp. nov., a nitrogen-fixing bacterium isolated from corn rhizosphere.
  • authors: Mehnaz S, Weselowski B, Lazarovits G
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.64804-0
  • year: 2007
  • mesh: Azospirillum/*classification/genetics/isolation & purification/physiology, Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, Nitrogen Fixation, Phylogeny, Plant Roots/microbiology, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Zea mays/*microbiology
  • topic2: Genetics

Reference

@idauthorstitledoi/urlID_cross_referencepubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
32008Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172826228776041
37813Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7475
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
91826Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID297130.1
117123Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109702Collection of Institut Pasteur (CIP 109702)