Strain identifier

BacDive ID: 134175

Type strain: Yes

Species: Flectobacillus lacus

Strain Designation: CL-GP79

Strain history: CIP <- 2006, JCM <- 2005, B.C. Cho, Seoul Univ., Seoul, Korea: strain CL-GP79

NCBI tax ID(s): 336819 (species)

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General

@ref: 31696

BacDive-ID: 134175

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped

description: Flectobacillus lacus CL-GP79 is an aerobe, Gram-negative, rod-shaped bacterium that was isolated from Highly eutrophic pond.

NCBI tax id

  • NCBI tax id: 336819
  • Matching level: species

strain history

@refhistory
67770B. C. Cho CL-GP79.
120539CIP <- 2006, JCM <- 2005, B.C. Cho, Seoul Univ., Seoul, Korea: strain CL-GP79

doi: 10.13145/bacdive134175.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Cytophagia
  • order: Cytophagales
  • family: Spirosomataceae
  • genus: Flectobacillus
  • species: Flectobacillus lacus
  • full scientific name: Flectobacillus lacus Hwang and Cho 2006

@ref: 31696

domain: Bacteria

phylum: Bacteroidetes

class: Cytophagia

order: Cytophagales

family: Cytophagaceae

genus: Flectobacillus

species: Flectobacillus lacus

strain designation: CL-GP79

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
31696negative4.7-10 µm0.3-0.6 µmrod-shapedno
120539negativerod-shapedno

pigmentation

  • @ref: 31696
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37513MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromatiyesDistilled water make up to (1000.000 ml);R2A agar (18.200 g)
120539CIP Medium 566yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566

culture temp

@refgrowthtypetemperaturerange
31696positivegrowth10-35
31696positiveoptimum25-30mesophilic
37513positivegrowth30mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
31696positivegrowth06-09alkaliphile
31696positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 31696
  • oxygen tolerance: aerobe

spore formation

  • @ref: 31696
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
31696NaClpositivegrowth0-0.5 %
31696NaClpositiveoptimum0-0.5 %

observation

  • @ref: 31696
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
31696506227N-acetylglucosamine+carbon source
3169615361pyruvate+carbon source
316964853esculin+hydrolysis
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
12053917632nitrate-reduction
12053916301nitrite-reduction

metabolite production

  • @ref: 120539
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
31696acid phosphatase+3.1.3.2
31696alkaline phosphatase+3.1.3.1
31696catalase+1.11.1.6
31696gelatinase+
31696cytochrome oxidase+1.9.3.1
120539oxidase+
120539catalase+1.11.1.6
120539urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120539-+++++++++++-+--++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120539---------+--+-----------------------------------+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
67770Highly eutrophic pondSeoulRepublic of KoreaKORAsia
120539Environment, Highly eutrophic pondGongdae Pond, located in the campus of Seoul National UniversityRepublic of KoreaKORAsia

taxonmaps

  • @ref: 69479
  • File name: preview.99_120737.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_200;96_30185;97_38148;98_49522;99_120737&stattab=map
  • Last taxonomy: Flectobacillus
  • 16S sequence: DQ112352
  • Sequence Identity:
  • Total samples: 1218
  • soil counts: 22
  • aquatic counts: 812
  • animal counts: 365
  • plant counts: 19

Safety information

risk assessment

  • @ref: 120539
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 31696
  • description: Flectobacillus lacus strain CL-GP79 16S ribosomal RNA gene, partial sequence
  • accession: DQ112352
  • length: 1369
  • database: nuccore
  • NCBI tax ID: 336819

GC content

@refGC-contentmethod
3169638.3
6777038.3high performance liquid chromatography (HPLC)

External links

@ref: 31696

culture collection no.: KCCM 42271, JCM 13398, CIP 109329, NCIMB 14446

straininfo link

  • @ref: 91773
  • straininfo: 297214

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16738091Flectobacillus lacus sp. nov., isolated from a highly eutrophic pond in Korea.Hwang CY, Cho BCInt J Syst Evol Microbiol10.1099/ijs.0.64071-02006Bacteroides/*classification/growth & development/*isolation & purification, Environment, Fresh Water/microbiology, Gram-Negative Bacteria/classification/isolation & purification, Korea, Molecular Sequence Data, Phylogeny, *Water MicrobiologyEnzymology
Phylogeny19622645Flectobacillus roseus sp. nov., isolated from freshwater in Taiwan.Sheu SY, Chiu TF, Cho NT, Chou JH, Sheu DS, Arun AB, Young CC, Chen CA, Wang JT, Chen WMInt J Syst Evol Microbiol10.1099/ijs.0.009738-02009Cytophagaceae/chemistry/classification/genetics/*isolation & purification, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry, Fresh Water/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, TaiwanGenetics
Phylogeny20008110Siphonobacter aquaeclarae gen. nov., sp. nov., a novel member of the family 'Flexibacteraceae', phylum Bacteroidetes.Tancsics A, Keki Z, Marialigeti K, Schumann P, Toth EMInt J Syst Evol Microbiol10.1099/ijs.0.019398-02009Base Composition, Cytophagaceae/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, Fatty Acids/metabolism, Fresh Water/*microbiology, Molecular Sequence Data, Phylogeny, Power Plants, RNA, Ribosomal, 16S/geneticsMetabolism
Phylogeny26297007Flectobacillus rhizosphaerae sp. nov., isolated from the rhizosphere soil of Oryza sativa (L.), and emended description of the genus Flectobacillus.Ramaprasad EVV, Sasikala C, Ramana CVInt J Syst Evol Microbiol10.1099/ijsem.0.0004322015Bacterial Typing Techniques, Base Composition, Cytophagaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, India, Molecular Sequence Data, Nucleic Acid Hybridization, Oryza/*microbiology, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny27902252Flectobacillus fontis sp. nov., isolated from a freshwater spring.Chen WM, Lin KR, Young CC, Sheu SYInt J Syst Evol Microbiol10.1099/ijsem.0.0016272017Bacterial Typing Techniques, Base Composition, Cytophagaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Natural Springs/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Taiwan, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny28056226Flectobacillus pallidus sp. nov., isolated from a freshwater spring.Sheu SY, Liu LP, Chen WMInt J Syst Evol Microbiol10.1099/ijsem.0.0017742017Bacterial Typing Techniques, Base Composition, Cytophagaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Natural Springs/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Taiwan, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorstitledoi/urlID_cross_referencepubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
31696Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172797328776041
37513Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7058
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
91773Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID297214.1
120539Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109329Collection of Institut Pasteur (CIP 109329)