Archive BacDiveID:10.13145/bacdive134095.20240916.9.1

Strain identifier

BacDive ID: 134095

Type strain:

Species: Tenuibacillus multivorans

Strain Designation: 28-1

NCBI tax ID(s): 237069 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 31272

BacDive-ID: 134095

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, motile, rod-shaped

description: Tenuibacillus multivorans 28-1 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from hypersaline environment.

NCBI tax id

NCBI tax id: 237069
Matching level: species

doi: 10.13145/bacdive134095.20240916.9.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/bacillota
domain: Bacteria
phylum: Bacillota
class: Bacilli
order: Caryophanales
family: Bacillaceae
genus: Tenuibacillus
species: Tenuibacillus multivorans
full scientific name: Tenuibacillus multivorans Ren and Zhou 2005

@ref: 31272

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Tenuibacillus

species: Tenuibacillus multivorans

strain designation: 28-1

type strain: yes

Morphology

cell morphology

@ref: 31272
gram stain: positive
cell length: 4 µm
cell width: 0.4 µm
cell shape: rod-shaped
motility: yes

Culture and growth conditions

culture temp

@ref	growth	type	temperature
31272	positive	growth	21-42
31272	positive	optimum	38.5

culture pH

@ref	ability	type	pH	PH range
31272	positive	growth	6.5-9	alkaliphile
31272	positive	optimum	7.5

Physiology and metabolism

oxygen tolerance

@ref: 31272
oxygen tolerance: aerobe

spore formation

@ref: 31272
spore formation: yes

halophily

@ref	salt	growth	tested relation	concentration
31272	NaCl	positive	growth	01-20 %
31272	NaCl	positive	optimum	5 %

metabolite utilization

@ref	Chebi-ID	metabolite	utilization activity	kind of utilization tested
31272	22599	arabinose	+	carbon source
31272	17057	cellobiose	+	carbon source
31272	28757	fructose	+	carbon source
31272	28260	galactose	+	carbon source
31272	17234	glucose	+	carbon source
31272	17754	glycerol	+	carbon source
31272	17716	lactose	+	carbon source
31272	17306	maltose	+	carbon source
31272	29864	mannitol	+	carbon source
31272	37684	mannose	+	carbon source
31272	28053	melibiose	+	carbon source
31272	16634	raffinose	+	carbon source
31272	26546	rhamnose	+	carbon source
31272	17992	sucrose	+	carbon source
31272	27082	trehalose	+	carbon source
31272	18222	xylose	+	carbon source
31272	4853	esculin	+	hydrolysis

metabolite production

@ref: 31272
Chebi-ID: 16136
metabolite: hydrogen sulfide
production: yes

enzymes

@ref	value	activity	ec
31272	catalase	+	1.11.1.6
31272	cytochrome oxidase	+	1.9.3.1

Isolation, sampling and environmental information

isolation

@ref: 31272
sample type: hypersaline environment

isolation source categories

Cat1: #Condition
Cat2: #Saline

taxonmaps

@ref: 69479
File name: preview.99_18400.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_8920;97_11299;98_13896;99_18400&stattab=map
Last taxonomy: Tenuibacillus multivorans subclade
16S sequence: AY319933
Sequence Identity:
Total samples: 21
soil counts: 7
aquatic counts: 6
animal counts: 6
plant counts: 2

Sequence information

16S sequences

@ref	description	accession	length	database	NCBI tax ID
31272	Tenuibacillus multivorans strain 28-1 16S ribosomal RNA gene, partial sequence	AY319933	1479	nuccore	237069
124043	Tenuibacillus multivorans gene for 16S rRNA, partial sequence, strain: NBRC 100370.	AB681155	1486	nuccore	237069

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Tenuibacillus multivorans NBRC 100370	GCA_007989445	contig	ncbi	237069
66792	Tenuibacillus multivorans strain NBRC 100370	237069.5	wgs	patric	237069

GC content

@ref: 31272
GC-content: 36.75

Genome-based predictions

predictions

@ref	trait	model	description	prediction	confidence	training_data
69480	gram-positive	gram-positive	Positive reaction to Gram-staining	yes	79.811	no
69480	anaerobic	anaerobic	Ability to grow under anoxygenic conditions (including facultative anaerobes)	no	95.386	yes
69480	spore-forming	spore-forming	Ability to form endo- or exospores	yes	75.496	no
69480	aerobic	aerobic	Ability to grow under oxygenic conditions (including facultative aerobes)	yes	86.578	yes
69480	thermophilic	thermophile	Ability to grow at temperatures above or equal to 45°C	no	86.303	yes
69480	flagellated	motile2+	Ability to perform flagellated movement	yes	80.334	no

External links

@ref: 31272

culture collection no.: AS 1.3442, NBRC 100370

straininfo link

@ref: 91703
straininfo: 135138

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Phylogeny	15653860	Tenuibacillus multivorans gen. nov., sp. nov., a moderately halophilic bacterium isolated from saline soil in Xin-Jiang, China.	Ren PG, Zhou PJ	Int J Syst Evol Microbiol	10.1099/ijs.0.63180-0	2005	Bacillaceae/chemistry/classification/genetics/isolation & purification, Bacterial Typing Techniques, China, DNA, Ribosomal, Fresh Water/microbiology, Genes, rRNA, Geologic Sediments/microbiology, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S, *Sodium Chloride	Genetics
Phylogeny	22941247	Tenuibacillus halotolerans sp. nov., a novel bacterium isolated from a soil sample from a salt lake in Xinjiang, China and emended description of the genus Tenuibacillus.	Gao XH, Gao S, Zhou Y, Guan HL, Zhang YJ, Jia M, Huang HW, Yang DX, Li WJ, Tang SK	Antonie Van Leeuwenhoek	10.1007/s10482-012-9802-3	2012	Bacillaceae/classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Hydrogen-Ion Concentration, Microscopy, Electron, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, *Soil Microbiology, Temperature, Vitamin K 2/analysis	Genetics

Reference

@id	authors	title	doi/url	ID_cross_reference	pubmed	journal
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ	10.1099/ijsem.0.004332
31272	Barberan A, Caceres Velazquez H, Jones S, Fierer N.	Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information	10.1128/mSphere.00237-17	27592	28776041
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)	https://diaspora-project.de/progress.html#genomes
66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg	BRENDA, the ELIXIR core data resource in 2021: new developments and updates	10.1093/nar/gkaa1025		33211880	Nucleic Acids Res. 49: D498-D508 2020
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.	MicrobeAtlas 1.0 beta	https://microbeatlas.org/
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)	https://diaspora-project.de/progress.html#genomes
91703	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.	StrainInfo: A central database for resolving microbial strain identifiers	10.60712/SI-ID135138.1
124043	Dr. Isabel Schober, Dr. Julia Koblitz	Data extracted from sequence databases, automatically matched based on designation and taxonomy