Strain identifier

BacDive ID: 133535

Type strain: Yes

Species: Ensifer numidicus

Strain history: CIP <- 2013, P. de Lajudie, Lab. Symbioses Tropicales et Méditerranéennes, UMR 113 IRD-CIRAD-SupAgro-Université Montpellier, Montpellier, France: strain ORS 1407

NCBI tax ID(s): 680248 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 8.1 (current version)

General

@ref: 29389

BacDive-ID: 133535

keywords: Bacteria, aerobe, Gram-negative, rod-shaped

description: Ensifer numidicus LMG 24690 is an aerobe, Gram-negative, rod-shaped bacterium that was isolated from Plant, Root nodule, Argyrolobium uniflorum.

NCBI tax id

  • NCBI tax id: 680248
  • Matching level: species

strain history

  • @ref: 121372
  • history: CIP <- 2013, P. de Lajudie, Lab. Symbioses Tropicales et Méditerranéennes, UMR 113 IRD-CIRAD-SupAgro-Université Montpellier, Montpellier, France: strain ORS 1407

doi: 10.13145/bacdive133535.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Rhizobiaceae
  • genus: Ensifer
  • species: Ensifer numidicus
  • full scientific name: Ensifer numidicus Merabet et al. 2010

  • synonyms

    • @ref: 20215
    • synonym: Sinorhizobium numidicum

@ref: 29389

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Hyphomicrobiales

family: Rhizobiaceae

genus: Ensifer

species: Ensifer numidicus

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
29389negativerod-shaped
121372negativerod-shapedyes

pigmentation

  • @ref: 29389
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
36543MEDIUM 705 - for EnsiferyesDistilled water make up to (1.000 l);Sodium chloride (0.050 g);Magnesiumsulphate heptahydrate (0.200 g);Calcium chloride dihydrate (0.040 g);Agar (15.000 g);Yeast extract (1.000 g);Potassium di-hydrogen phosphate (0.500 g);Sodium glutamate (0.500 g);Ferri
121372CIP Medium 705yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=705

culture temp

@refgrowthtypetemperaturerange
29389positivegrowth28-40
36543positivegrowth30mesophilic

culture pH

  • @ref: 29389
  • ability: positive
  • type: growth
  • pH: 06-09
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29389aerobe
121372obligate aerobe

spore formation

  • @ref: 29389
  • spore formation: no

halophily

  • @ref: 29389
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 01-02 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2938916449alanine+carbon source
2938935391aspartate+carbon source
2938924265gluconate+carbon source
2938927570histidine+carbon source
2938917272propionate+carbon source
2938930031succinate+carbon source
12137217632nitrate+reduction
12137216301nitrite+reduction

metabolite production

  • @ref: 121372
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
121372oxidase+
121372catalase+1.11.1.6
121372urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121372-++--+-++--++--+-+--

Isolation, sampling and environmental information

isolation

  • @ref: 121372
  • sample type: Plant, Root nodule, Argyrolobium uniflorum
  • geographic location: South
  • country: Tunisia
  • origin.country: TUN
  • continent: Africa
  • isolation date: 1993

Safety information

risk assessment

  • @ref: 121372
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

GC content

  • @ref: 29389
  • GC-content: 62.8

External links

@ref: 29389

culture collection no.: LMG 24690, CIP 109850, ORS 1407

straininfo link

  • @ref: 91491
  • straininfo: 97969

literature

  • topic: Phylogeny
  • Pubmed-ID: 19656922
  • title: Multilocus sequence analysis of root nodule isolates from Lotus arabicus (Senegal), Lotus creticus, Argyrolobium uniflorum and Medicago sativa (Tunisia) and description of Ensifer numidicus sp. nov. and Ensifer garamanticus sp. nov.
  • authors: Merabet C, Martens M, Mahdhi M, Zakhia F, Sy A, Le Roux C, Domergue O, Coopman R, Bekki A, Mars M, Willems A, de Lajudie P
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.012088-0
  • year: 2009
  • mesh: Bacterial Proteins/genetics, Bacterial Typing Techniques, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fabaceae/*microbiology, Lotus/*microbiology, Medicago/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizobiaceae/*classification/*genetics/isolation & purification/metabolism, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlID_cross_referencepubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
29389Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172579628776041
36543Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7641
68382Automatically annotated from API zym
91491Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID97969.1
121372Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109850Collection of Institut Pasteur (CIP 109850)