Strain identifier

BacDive ID: 133400

Type strain: Yes

Species: Desulfuribacillus stibiiarsenatis

Strain Designation: MLFW-2

Strain history: C. A. Abin; Univ. of Georgia, USA; MLFW-2.

NCBI tax ID(s): 1390249 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 8.1 (current version)

General

@ref: 64149

BacDive-ID: 133400

DSM-Number: 28709

keywords: genome sequence, 16S sequence, Bacteria, obligate anaerobe, spore-forming, Gram-negative, motile, rod-shaped

description: Desulfuribacillus stibiiarsenatis MLFW-2 is an obligate anaerobe, spore-forming, Gram-negative bacterium that was isolated from arsenic-rich anoxic sediments from drainage area of a geothermal; spring.

NCBI tax id

  • NCBI tax id: 1390249
  • Matching level: species

strain history

@refhistory
64149<- C. Abin, University of Georgia, Athens, USA ; MLFW-2
67770C. A. Abin; Univ. of Georgia, USA; MLFW-2.

doi: 10.13145/bacdive133400.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Desulfuribacillaceae
  • genus: Desulfuribacillus
  • species: Desulfuribacillus stibiiarsenatis
  • full scientific name: Desulfuribacillus stibiiarsenatis Abin and Hollibaugh 2017

@ref: 64149

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacilli, not assigned to order

family: Caryophanales, not assigned to family

genus: Desulfuribacillus

species: Desulfuribacillus stibiiarsenatis

full scientific name: Desulfuribacillus stibiiarsenatis Abin and Hollibaugh 2017

strain designation: MLFW-2

type strain: yes

Morphology

cell morphology

  • @ref: 25255
  • gram stain: negative
  • cell length: 2.0-11.0 µm
  • cell width: 0.3-0.5 µm
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
25255basal salts medium 2 (BSM-2)yes
25255basal salts medium 3 (BSM-3)yes
25255basal salts medium 4 (BSM-4)yes
64149BSM MEDIUM (DSMZ Medium 1663)yeshttps://mediadive.dsmz.de/medium/1663Name: BSM medium (DSMZ Medium 1663) Composition: NaHCO3 4.2 g/l NaCl 3.62 g/l Na-DL-lactate 1.12 g/l NaNO3 0.85 g/l K2HPO4 0.26 g/l Yeast extract 0.2 g/l (NH4)Cl 0.08 g/l Na2SO4 0.05 g/l MgCl2 x 6 H2O 0.003 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l Pyridoxine hydrochloride 0.0001 g/l CaCl2 x 2 H2O 0.0001 g/l ZnCl2 7e-05 g/l Thiamine HCl 5e-05 g/l (DL)-alpha-Lipoic acid 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Calcium D-(+)-pantothenate 5e-05 g/l Nicotinic acid 5e-05 g/l Riboflavin 5e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Vitamin B12 1e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
25255positivegrowth10-43
25255positiveoptimum34mesophilic
64149positivegrowth35mesophilic
67770positivegrowth34mesophilic

culture pH

@refabilitytypepHPH range
25255positivegrowth7.0-10.0alkaliphile
25255positiveoptimum8.25-8.50

Physiology and metabolism

oxygen tolerance

  • @ref: 25255
  • oxygen tolerance: obligate anaerobe

spore formation

  • @ref: 25255
  • spore description: terminal endospores
  • type of spore: endospore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
25255NaClpositivegrowth0.0-5.0 %(w/v)
25255NaCloptimum0.75 %(w/v)

observation

@refobservation
25255no outer membrane present
67770quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2525530089acetate-electron donor
25255amorphous fe(iii) oxyhydroxid-electron acceptor
2525522651ascorbate-electron donor
25255casein-hydrolysis
2525535404chromate-electron acceptor
2525516947citrate-electron donor
2525515824D-fructose-electron donor
2525512936D-galactose-electron donor
2525517634D-glucose-electron donor
2525517924D-sorbitol-electron donor
2525533403elemental sulfur-electron acceptor
2525516236ethanol-electron donor
2525531605ferric citrate-electron acceptor
2525529806fumarate-electron acceptor
2525529806fumarate-electron donor
2525517754glycerol-electron donor
2525515428glycine-electron donor
2525529805glycolate-electron donor
2525529985L-glutamate-electron donor
2525517115L-serine-electron donor
2525517895L-tyrosine-hydrolysis
2525518300maleic acid-electron donor
2525525115malate-electron donor
25255manganese dioxide-electron acceptor
2525517790methanol-electron donor
2525536264molybdate-electron acceptor
2525530623oxalate-electron donor
2525517272propionate-electron donor
2525528017starch-hydrolysis
2525530031succinate-electron donor
2525516189sulfate-electron acceptor
2525517359sulfite-electron acceptor
25255132950tartrate-electron donor
2525515226tetrathionate-electron acceptor
2525516094thiosulfate-electron acceptor
2525515724trimethylamine n-oxide-electron acceptor
2525553424tween 20-hydrolysis
2525546442vanadate-electron acceptor
2525530089acetate+carbon source
2525530295antimonate+electron acceptor
2525529125arsenate+electron acceptor
2525528262dimethyl sulfoxide+electron acceptor
2525518276diydrogen+electron donor
2525515740formate+electron donor
2525524996lactate+electron donor
2525517632nitrate+electron acceptor
2525516301nitrite+electron acceptor
2525515361pyruvate+electron donor
2525515075selenate+electron acceptor
2525518212selenite+electron acceptor
2525553423tween 40+hydrolysis
2525553426tween 80+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
2525517698chloramphenicolyesyes5 µg/mL
252556104kanamycinyesyes25 µg/mL
25255100147nalidixic acidyesyes75 µg/mL
2525528971ampicillinyesyes5 µg/mL
2525528077rifampicinyesyes5 µg/mL
2525548923erythromycinyesyes5 µg/mL
2525517833gentamicinyesyes5 µg/mL
2525517076streptomycinyesyes5 µg/mL

enzymes

@refvalueactivityec
25255catalase+1.11.1.6
25255cytochrome oxidase-1.9.3.1

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    25255C16:021.7
    25255C16:1ω5c1.3
    25255C16:1ω7c13.9
    25255C16:1ω9c14.1
    25255C18:01.2
    25255C18:1ω5c1.5
    25255C18:1ω7c35.4
    25255C18:1ω9c7.3
  • type of FA analysis: whole cell analysis
  • incubation medium: basal salts medium 5 (BSM-5)
  • agar/liquid: liquid
  • incubation temperature: 34
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture composition
25255arsenic-rich anoxic sediments from drainage area of a geothermal springMono Lake, CaliforniaUSAUSANorth America37-119basal salts medium (BSM-1)supplemented with 1 mM lactate and 2 mM Sb(V)
64149arsenic-rich anoxic sediment from the drainage area of a geothermal springCalifornia, Mono Lake (37° 56' 28.7'' N 119° 1' 22.4" W)USAUSANorth America37.9413-119.023
67770Anaerobic sediments from the drainage area of a geothermal spring near Mono LakeCAUSAUSANorth America

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Environmental#Terrestrial#Sediment
#Condition#Anoxic (anaerobic)
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_6661.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_591;96_3158;97_3868;98_4913;99_6661&stattab=map
  • Last taxonomy: Desulfuribacillus stibiiarsenatis subclade
  • 16S sequence: KF387535
  • Sequence Identity:
  • Total samples: 426
  • soil counts: 91
  • aquatic counts: 121
  • animal counts: 169
  • plant counts: 45

Safety information

risk assessment

  • @ref: 64149
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 64149
  • description: Desulfuribacillus stibiiarsenatis strain MLFW-2 16S ribosomal RNA gene, partial sequence
  • accession: KF387535
  • length: 1446
  • database: ena
  • NCBI tax ID: 1390249

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Desulfuribacillus stibiiarsenatis MLFW-22751185656draftimg1390249
67770Desulfuribacillus stibiiarsenatis MLFW-2GCA_001742305contigncbi1390249

GC content

@refGC-contentmethod
6414938.2sequence analysis
6777038.2genome sequence analysis

External links

@ref: 64149

culture collection no.: DSM 28709, JCM 30866, ATCC PTA-120556

straininfo link

  • @ref: 91426
  • straininfo: 397858

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27974090Desulfuribacillus stibiiarsenatis sp. nov., an obligately anaerobic, dissimilatory antimonate- and arsenate-reducing bacterium isolated from anoxic sediments, and emended description of the genus Desulfuribacillus.Abin CA, Hollibaugh JTInt J Syst Evol Microbiol10.1099/ijsem.0.0017322017Arsenates/metabolism, Bacillales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, California, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Natural Springs/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Metabolism30548120Transcriptional response of the obligate anaerobe Desulfuribacillus stibiiarsenatis MLFW-2(T) to growth on antimonate and other terminal electron acceptors.Abin CA, Hollibaugh JTEnviron Microbiol10.1111/1462-2920.145032019Arsenates/metabolism, Bacillales/*genetics/*growth & development/isolation & purification, Bacterial Proteins/*genetics/metabolism, Electrons, Nitrate Reductase/genetics/metabolism, Nitrates/metabolism, Oxidation-Reduction, Oxidoreductases/genetics/metabolism, Phylogeny, Transcription, GeneticEnzymology

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25255C. A. H. Abin, J. T.Desulfuribacillus stibiiarsenatis sp. nov., an obligately anaerobic, dissimilatory antimonate- and arsenate-reducing bacterium isolated from anoxic sediments, and emended description of the genus Desulfuribacillus10.1099/ijsem.0.001732IJSEM 67: 1011-1017 201727974090
64149Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28709Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28709)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
91426Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397858.1