Strain identifier

BacDive ID: 133389

Type strain: Yes

Species: Chryseobacterium lineare

Strain Designation: XC0022

Strain history: <- Zhe Zhao, Zhejiang Univ.

NCBI tax ID(s): 1859201 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 8.1 (current version)

General

@ref: 25281

BacDive-ID: 133389

keywords: 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative, rod-shaped, colony-forming, pigmented

description: Chryseobacterium lineare XC0022 is an aerobe, psychrophilic, Gram-negative bacterium that forms circular colonies and has a yellow pigmentation.

NCBI tax id

  • NCBI tax id: 1859201
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Zhe Zhao, Zhejiang Univ.

doi: 10.13145/bacdive133389.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Weeksellaceae
  • genus: Chryseobacterium
  • species: Chryseobacterium lineare
  • full scientific name: Chryseobacterium lineare Zhao et al. 2017

@ref: 25281

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Chryseobacterium

species: Chryseobacterium lineare

strain designation: XC0022

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
25281negative2.0-3.0 µm0.8-1.0 µmrod-shapedno
67771rod-shapedno
67771negative

colony morphology

  • @ref: 25281
  • colony color: orange-yellow
  • colony shape: circular

pigmentation

  • @ref: 25281
  • production: yes
  • color: yellow
  • name: Flexirubin-type

Culture and growth conditions

culture medium

@refnamegrowthcomposition
25281PTYGyes5 g/l tryptone,5 g/l soya peptone, 10 g/l yeast extract, 10 g/l glucose, 1 ml Tween80, 0.05 g/l L-cysteine hydrochloride, 0.8 mg/l CaCl2, 4 mg/l K2HPO4, 4 mg/l KH2PO4, 1.92 mg/l MgSO4x7H2O, 40 mg/l Na2CO3, 8 mg/l NaCl, 15 g/l agar, pH 6.8–7.0
25281TSByes
25281nutritient agar (NA)yes
25281MacConkey agar (BD)no

culture temp

@refgrowthtypetemperaturerange
25281positivegrowth4psychrophilic
25281nogrowth43thermophilic
25281positiveoptimum30mesophilic
67771positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
25281positivegrowth5.0-10.0alkaliphile
25281positiveoptimum6.0-6.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
25281aerobe
67771aerobe

spore formation

  • @ref: 25281
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
25281NaClpositivegrowth0.0-4.0 %(w/v)
25281NaCloptimum0 %(w/v)

observation

  • @ref: 67771
  • observation: quinones: MK-6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2528130089acetate-carbon source
2528116947citrate-carbon source
2528112936D-galactose-carbon source
2528115740formate-carbon source
2528124265gluconate-carbon source
2528117234glucose-fermentation
2528117754glycerol-carbon source
2528124996lactate-carbon source
2528125115malate-carbon source
2528117632nitrate-reduction
2528115361pyruvate-carbon source
2528130911sorbitol-carbon source
2528127082trehalose-carbon source
2528116199urea-hydrolysis
2528127613amygdalin+builds acid from
2528118305arbutin+builds acid from
2528117057cellobiose+carbon source
2528117057cellobiose+builds acid from
2528117108D-arabinose+builds acid from
2528115824D-fructose+carbon source
2528115824D-fructose+builds acid from
2528112936D-galactose+builds acid from
2528116024D-mannose+carbon source
2528165327D-xylose+carbon source
252814853esculin+hydrolysis
25281esculin ferric citrate+builds acid from
2528116236ethanol+carbon source
252815291gelatin+hydrolysis
2528130849L-arabinose+carbon source
2528162345L-rhamnose+carbon source
2528117266L-sorbose+builds acid from
2528117716lactose+carbon source
2528117306maltose+carbon source
2528128053melibiose+builds acid from
25281506227N-acetylglucosamine+builds acid from
2528132032potassium gluconate+builds acid from
2528116634raffinose+carbon source
2528117814salicin+builds acid from
2528128017starch+carbon source
2528128017starch+builds acid from
2528117992sucrose+carbon source
2528153424tween 20+hydrolysis
2528153423tween 40+hydrolysis
2528153425tween 60+hydrolysis
2528153426tween 80+hydrolysis
2528117151xylitol+builds acid from

metabolite production

@refChebi-IDmetaboliteproduction
2528115688acetoinno
2528135581indoleno
2528116136hydrogen sulfideyes

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-test
2528117234glucose-
2528115688acetoin-

enzymes

@refvalueactivityec
25281acid phosphatase+3.1.3.2
25281alkaline phosphatase+3.1.3.1
25281alpha-galactosidase-3.2.1.22
25281alpha-glucosidase+3.2.1.20
25281alpha-mannosidase-3.2.1.24
25281arginine dihydrolase-3.5.3.6
25281beta-fucosidase-
25281beta-galactosidase+3.2.1.23
25281beta-glucosidase+3.2.1.21
25281beta-glucuronidase-3.2.1.31
25281catalase+1.11.1.6
25281chymotrypsin+3.4.4.5
25281cystine arylamidase+3.4.11.3
25281cytochrome oxidase+1.9.3.1
25281esterase (C 4)+
25281esterase Lipase (C 8)+
25281leucine arylamidase+3.4.11.1
25281lipase (C 14)-
25281N-acetyl-beta-glucosaminidase+3.2.1.52
25281naphthol-AS-BI-phosphohydrolase+
25281trypsin+3.4.21.4
25281valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    25281C16:06.5
    25281C16:0 3OH1.2
    25281C18:1ω7c1.1
    25281C18:1ω9c14.2
    25281C15:0 iso26.5
    25281iso-C15:0 2-OH/C16:1ω7c10.6
    25281C15:0 iso 3OH2.8
    25281C17:0 iso4.4
    25281C17:0 iso 3OH15.3
    25281C17:1 iso ω9c6.6
    25281Unknown3.814
  • type of FA analysis: whole cell analysis
  • incubation medium: PTYG
  • agar/liquid: liquid
  • incubation temperature: 30
  • incubation time: 1
  • software version: Sherlock 4.5
  • library/peak naming table: TSBA 40
  • system: MIS MIDI
  • instrument: 6890N; Agilent
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
25281freshwater samplestream in Zhejiang provinceChinaCHNAsia30119PTYG3 days30standard dilution plating technique
67771From freshwater, clean limpid streamLinan City, Zhejiang ProvinceChinaCHNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Freshwater

taxonmaps

  • @ref: 69479
  • File name: preview.99_5511.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_76;96_869;97_3271;98_4115;99_5511&stattab=map
  • Last taxonomy: Chryseobacterium
  • 16S sequence: KX185933
  • Sequence Identity:
  • Total samples: 540
  • soil counts: 122
  • aquatic counts: 220
  • animal counts: 68
  • plant counts: 130

Sequence information

16S sequences

  • @ref: 25281
  • description: Chryseobacterium sp. XC0022 16S ribosomal RNA gene, partial sequence
  • accession: KX185933
  • length: 1482
  • database: nuccore
  • NCBI tax ID: 1859201

GC content

  • @ref: 25281
  • GC-content: 29.7
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 25281

culture collection no.: KCTC 52364, MCCC 1K02723

literature

  • topic: Phylogeny
  • Pubmed-ID: 27902240
  • title: Chryseobacterium lineare sp. nov., isolated from a limpid stream.
  • authors: Zhao Z, Tu YQ, Shen X, Han SB, Zhang CY, Sun C, Wu M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001629
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, China, Chryseobacterium/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Nucleic Acid Hybridization, Phosphatidylethanolamines/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rivers/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25281Z. T. Zhao, Y. Q.,Shen, X.,Han, S. B.,Zhang, C. Y.,Sun, C.,Wu, M.Chryseobacterium lineare sp. nov., isolated from a limpid stream10.1099/ijsem.0.001629IJSEM 67: 800-805 201727902240
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/