Strain identifier

BacDive ID: 133386

Type strain: Yes

Species: Mucilaginibacter psychrotolerans

Strain Designation: NH7-4

Strain history: Y. Liu; Inst. of Tibetan Plateau Res., CAS, China; NH7-4.

NCBI tax ID(s): 1524096 (species)

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General

@ref: 25282

BacDive-ID: 133386

keywords: genome sequence, 16S sequence, Bacteria, Gram-negative, rod-shaped, colony-forming

description: Mucilaginibacter psychrotolerans NH7-4 is a Gram-negative, rod-shaped bacterium that forms circular colonies.

NCBI tax id

  • NCBI tax id: 1524096
  • Matching level: species

strain history

  • @ref: 67770
  • history: Y. Liu; Inst. of Tibetan Plateau Res., CAS, China; NH7-4.

doi: 10.13145/bacdive133386.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Sphingobacteriia
  • order: Sphingobacteriales
  • family: Sphingobacteriaceae
  • genus: Mucilaginibacter
  • species: Mucilaginibacter psychrotolerans
  • full scientific name: Mucilaginibacter psychrotolerans Deng et al. 2017

@ref: 25282

domain: Bacteria

phylum: Bacteroidetes

class: Sphingobacteriia

order: Sphingobacteriales

family: Sphingobacteriaceae

genus: Mucilaginibacter

species: Mucilaginibacter psychrotolerans

strain designation: NH7-4

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
25282negative2 µm0.6 µmrod-shapedno
69480no90.444
69480negative99.986

colony morphology

  • @ref: 25282
  • colony color: pink
  • colony shape: circular
  • medium used: R2A agar

Culture and growth conditions

culture medium

  • @ref: 25282
  • name: Reasoner's 2A agar (R2A)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
25282positivegrowth0-30
25282positiveoptimum20psychrophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
25282positivegrowth5.0-9.0alkaliphile
25282positiveoptimum7

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.741

halophily

  • @ref: 25282
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-1 %(w/v)

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2528217128adipate-assimilation
2528222599arabinose-assimilation
2528216947citrate-assimilation
2528227689decanoate-assimilation
252825291gelatin-hydrolysis
2528224265gluconate-assimilation
2528217234glucose-fermentation
2528229864mannitol-assimilation
2528217632nitrate-reduction
2528218401phenylacetate-assimilation
2528227897tryptophan-energy source
252824853esculin+hydrolysis

metabolite production

  • @ref: 25282
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitemethylred-testindole test
2528217234glucose-
2528235581indole-

enzymes

@refvalueactivityec
25282acid phosphatase+3.1.3.2
25282alpha-chymotrypsin-3.4.21.1
25282alpha-fucosidase+3.2.1.51
25282alpha-galactosidase+3.2.1.22
25282alpha-glucosidase+3.2.1.20
25282alpha-mannosidase+3.2.1.24
25282arginine dihydrolase-3.5.3.6
25282beta-galactosidase+3.2.1.23
25282beta-glucosidase+3.2.1.21
25282beta-glucuronidase-3.2.1.31
25282catalase+1.11.1.6
25282cystine arylamidase+3.4.11.3
25282cytochrome oxidase+1.9.3.1
25282esterase (C 4)+
25282esterase Lipase (C 8)+
25282leucine arylamidase+3.4.11.1
25282lipase (C 14)-
25282N-acetyl-beta-glucosaminidase+3.2.1.52
25282naphthol-AS-BI-phosphohydrolase+
25282nitrate reductase-1.7.99.4
25282trypsin+3.4.21.4
25282tryptophan deaminase-4.1.99.1
25282urease-3.5.1.5
25282valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    25282C15:0 anteiso1.2
    25282C16:07.8
    25282C16:1ω5c8.5
    25282C16:1ω7c / C15:0 iso 2OH49.8
    25282C17:1ω8c1.4
    25282C15:0 iso14
    25282C17:0 iso 3OH6.3
    25282C17:1 iso ω9c2.9
  • type of FA analysis: whole cell analysis
  • incubation medium: See reference
  • incubation temperature: 10
  • incubation time: 10
  • software version: Sherlock 6.0
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperaturesample type
25282Riganqiao peatlands, Tibetan PlateauChinaCHNAsia33102R2A3 days20
67770Riganqiao peatlands on the Tibetan Plateau

Sequence information

16S sequences

  • @ref: 25282
  • description: Mucilaginibacter sp. NH7-4 16S ribosomal RNA gene, partial sequence
  • accession: KJ755879
  • length: 1427
  • database: nuccore
  • NCBI tax ID: 1524096

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mucilaginibacter psychrotolerans NH7-4GCA_004519315scaffoldncbi1524096
66792Mucilaginibacter psychrotolerans NH7-42881113850draftimg1524096

GC content

  • @ref: 67770
  • GC-content: 48.6
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno89.803no
flagellatedno97.486yes
gram-positiveno97.346yes
anaerobicno99.242no
aerobicyes87.611no
halophileno95.946yes
spore-formingno92.79no
glucose-utilyes86.666no
thermophileno99.124yes
glucose-fermentno90.974yes

External links

@ref: 25282

culture collection no.: JCM 30607, CGMCC 1.14937

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27902239Mucilaginibacterpsychrotolerans sp. nov., isolated from peatlands.Deng Y, Shen L, Xu B, Liu Y, Gu Z, Liu H, Zhou YInt J Syst Evol Microbiol10.1099/ijsem.0.0015602017Bacterial Typing Techniques, Bacteroidetes/*classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phosphatidylethanolamines/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Tibet, Vitamin K 2/analogs & derivatives/chemistry, *WetlandsTranscriptome
Phylogeny31334699Mucilaginibacter terrenus sp. nov., isolated from manganese mine soil.Zhou Z, Dong Y, Xia X, Wu S, Huang Y, Liao S, Wang GInt J Syst Evol Microbiol10.1099/ijsem.0.0035922019Bacterial Typing Techniques, Bacteroidetes/*classification/isolation & purification, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Manganese, *Mining, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25282Y. S. Deng, L.,Xu, B.,Liu, Y.,Gu, Z.,Liu, H.,Zhou, Y.Mucilaginibacter psychrotolerans sp. nov., isolated from peatlands10.1099/ijsem.0.001560IJSEM 67: 767-771 201727902239
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1