Strain identifier

BacDive ID: 133381

Type strain: Yes

Species: Pseudaeromonas pectinilytica

Strain Designation: AR1

Strain history: J.-C. Chae; Div. of Biotechnol., Chonbuk Natl. Univ., South Korea; AR1.

NCBI tax ID(s): 1756761 (species)

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General

@ref: 67671

BacDive-ID: 133381

DSM-Number: 112297

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped, colony-forming

description: Pseudaeromonas pectinilytica AR1 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from water sample from a freshwater stream in Jeonju.

NCBI tax id

  • NCBI tax id: 1756761
  • Matching level: species

strain history

@refhistory
67671<- JCM; JCM 31503 <- Chonbuk Nat. Univ., Jeonju-si, Koreaq; AR1
67770J.-C. Chae; Div. of Biotechnol., Chonbuk Natl. Univ., South Korea; AR1.

doi: 10.13145/bacdive133381.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Aeromonadales
  • family: Aeromonadaceae
  • genus: Pseudaeromonas
  • species: Pseudaeromonas pectinilytica
  • full scientific name: Pseudaeromonas pectinilytica Padakandla and Chae 2017

@ref: 67671

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Aeromonadales

family: Aeromonadaceae

genus: Pseudaeromonas

species: Pseudaeromonas pectinilytica

full scientific name: Pseudaeromonas pectinilytica Padakandla and Chae 2017

strain designation: AR1

type strain: yes

Morphology

cell morphology

  • @ref: 25256
  • gram stain: negative
  • cell length: 1.5-2.5 µm
  • cell width: 0.5-1.0 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: polar

colony morphology

  • @ref: 25256
  • colony color: translucent
  • colony shape: circular
  • medium used: Mueller Hinton agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
25256Mueller Hinton agaryes
25256Mueller Hinton brothyes
67671NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
25256positivegrowth10-45
67671positivegrowth28mesophilic
67770positivegrowth30mesophilic

culture pH

  • @ref: 25256
  • ability: positive
  • type: growth
  • pH: 6.0-8.0

Physiology and metabolism

oxygen tolerance

  • @ref: 25256
  • oxygen tolerance: aerobe

spore formation

  • @ref: 25256
  • type of spore: endospore
  • spore formation: no

halophily

  • @ref: 25256
  • salt: NaCl
  • tested relation: maximum
  • concentration: 1.5 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
25256739183-O-methyl-D-glucose-carbon source
2525627613amygdalin-carbon source
2525616947citrate-assimilation
252565291gelatin-hydrolysis
2525637684mannose-carbon source
2525617632nitrate-reduction
2525617814salicin-carbon source
2525653423tween 40-hydrolysis
2525616199urea-hydrolysis
2525618305arbutin+carbon source
2525617057cellobiose+carbon source
2525615824D-fructose+carbon source
2525678697D-fructose 6-phosphate+carbon source
2525612936D-galactose+carbon source
2525617634D-glucose+carbon source
2525617634D-glucose+fermentation
2525616899D-mannitol+carbon source
2525616024D-mannose+carbon source
2525623652dextrin+carbon source
252564853esculin+hydrolysis
2525617234glucose+fermentation
2525632323glucuronamide+carbon source
2525628087glycogen+carbon source
2525617596inosine+carbon source
2525630849L-arabinose+carbon source
2525617716lactose+carbon source
2525617306maltose+carbon source
25256320055methyl beta-D-glucopyranoside+carbon source
25256506227N-acetylglucosamine+carbon source
2525617309pectin+hydrolysis
2525617309pectin+carbon source
2525633942ribose+carbon source
2525628017starch+hydrolysis
2525628017starch+carbon source
2525617992sucrose+carbon source
2525613705acetoacetate+/-carbon source
2525628066gentiobiose+/-carbon source
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose+fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.
2525629673rifamycin svyesyes
252566472lincomycinyesyes15 µg (disc)
2525675273niaproofyesyes
25256100147nalidixic acidyesyes
2525675248potassium telluriteyesyes
25256161680aztreonamyesyes
252569332sulfamethoxazoleyesyes
2525618208penicillin gyesyes10 µg (disc)
252563745clindamycinyesyes2 µg (disc)
2525628971ampicillinyesyes10 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
2525615688acetoinno
2525616136hydrogen sulfideno
2525635581indoleno
6836935581indoleno

metabolite tests

@refChebi-IDmetabolitemethylred-testindole test
2525617234glucose+
6836935581indole-

enzymes

@refvalueactivityec
25256arginine dihydrolase-3.5.3.6
25256beta-galactosidase-3.2.1.23
25256catalase-1.11.1.6
25256cytochrome oxidase-1.9.3.1
25256lysine decarboxylase-4.1.1.18
25256ornithine decarboxylase-4.1.1.17
25256tryptophan deaminase4.1.99.1
68369cytochrome oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    25256C12:07.3
    25256C14:02.8
    25256C14:0 3OH / C16:1 iso7.2
    25256C16:035.8
    25256C16:1ω7c / C16:1ω6c37.4
    25256C18:01.1
    25256C18:1ω7c8.4
  • type of FA analysis: whole cell analysis
  • incubation medium: Mueller Hinton agar
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 2
  • software version: Sherlock 6.0
  • system: MIS MIDI
  • method/protocol: Sasser 2001

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
67671--+--+-+-+-+++-------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
25256water sample from a freshwater stream in Jeonjufreshwater stream in Jeonju, South KoreaRepublic of KoreaKORAsia
67671freshwater streamJeonjuRepublic of KoreaKORAsia35.8167127.15
67770Freshwater stream in JeonjuRepublic of KoreaKORAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Freshwater

taxonmaps

  • @ref: 69479
  • File name: preview.99_53529.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_11;96_8819;97_19412;98_38320;99_53529&stattab=map
  • Last taxonomy: Pseudaeromonas pectinilytica
  • 16S sequence: KT184595
  • Sequence Identity:
  • Total samples: 267
  • soil counts: 9
  • aquatic counts: 237
  • animal counts: 19
  • plant counts: 2

Safety information

risk assessment

  • @ref: 67671
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
2525616S rRNA gene, dnaJ and cpn60-UT sequencesKT1845951480ena1756761
2525616S rRNA gene, dnaJ and cpn60-UT sequencesLT601553898ena1756761
2525616S rRNA gene, dnaJ and cpn60-UT sequencesLT601554718ena1756761

GC content

@refGC-contentmethod
2525654.7Thermal denaturation, fluorometry
6777054.7thermal denaturation, midpoint method (Tm)

External links

@ref: 67671

culture collection no.: KCTC 42754, JCM 31503, DSM 112297

straininfo link

  • @ref: 91421
  • straininfo: 399569

literature

  • topic: Phylogeny
  • Pubmed-ID: 27974085
  • title: Reclassification of Aeromonas sharmana to a new genus as Pseudaeromonas sharmana gen. nov., comb. nov., and description of Pseudaeromonas pectinilytica sp. nov. isolated from a freshwater stream.
  • authors: Padakandla SR, Chae JC
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001734
  • year: 2017
  • mesh: Aeromonadaceae/*classification/genetics/isolation & purification, Aeromonas/*classification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rivers/*microbiology, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25256S. R. C. Padakandla, J. C.Reclassification of Aeromonas sharmana to a new genus as Pseudaeromonas sharmana gen. nov., comb. nov., and description of Pseudaeromonas pectinilytica sp. nov. isolated from a freshwater stream10.1099/ijsem.0.001734IJSEM 67: 1018-1023 201727974085
67671Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-112297Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 112297)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
91421Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID399569.1