Strain identifier

BacDive ID: 133353

Type strain: Yes

Species: Saccharedens versatilis

Strain Designation: CAG32

NCBI tax ID(s): 1680761 (species)

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General

@ref: 25210

BacDive-ID: 133353

DSM-Number: 100909

keywords: 16S sequence, Bacteria, obligate aerobe, Gram-negative, motile, rod-shaped

description: Saccharedens versatilis CAG32 is an obligate aerobe, Gram-negative, motile bacterium that was isolated from gut of the arboreal ant Cephalotes rohweri.

NCBI tax id

  • NCBI tax id: 1680761
  • Matching level: species

doi: 10.13145/bacdive133353.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Alcaligenaceae
  • genus: Saccharedens
  • species: Saccharedens versatilis
  • full scientific name: Saccharedens versatilis Lin et al. 2017

@ref: 25210

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Alcaligenaceae

genus: Saccharedens

species: Saccharedens versatilis

strain designation: CAG32

type strain: yes

Morphology

cell morphology

  • @ref: 25210
  • gram stain: negative
  • cell length: 0.8-1.6 µm
  • cell width: 0.3-0.4 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 25210
  • type of hemolysis: gamma
  • colony color: pale white

Culture and growth conditions

culture medium

@refnamegrowth
25210brain heart infusion (BHI) agaryes
25210blood agaryes
25210solid trypticase soy mediumyes
25210liquid trypticase soy mediumyes

culture temp

@refgrowthtypetemperaturerange
25210positivegrowth23.0-47.0
25210positiveoptimum40thermophilic

culture pH

@refabilitytypepHPH range
25210positivegrowth4.0-8.0acidophile
25210positiveoptimum7.0-7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 25210
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
25210NaClpositivegrowth0.0-2.0 %(w/v)
25210NaCloptimum0.5 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2521030089acetate-respiration
2521036219alpha-lactose-respiration
2521017126carnitine-respiration
2521017057cellobiose-respiration
2521015740formate-respiration
2521016977L-alanine-respiration
2521017196L-asparagine-respiration
2521029991L-aspartate-respiration
2521018287L-fucose-respiration
2521015971L-histidine-respiration
2521015603L-leucine-respiration
252106359lactulose-respiration
2521028053melibiose-respiration
2521017309pectin-respiration
2521017272propionate-respiration
2521016634raffinose-respiration
2521017992sucrose-respiration
2521017151xylitol-respiration
25210739183-O-methyl-D-glucose+respiration
2521030089acetate+respiration
2521015963ribitol+respiration
2521016947citrate+respiration
2521018333D-arabitol+respiration
2521015824D-fructose+respiration
2521078697D-fructose 6-phosphate+respiration
2521028847D-fucose+respiration
2521012936D-galactose+respiration
252108391D-gluconate+respiration
2521017634D-glucose+respiration
2521014314D-glucose 6-phosphate+respiration
2521016024D-mannose+respiration
2521016523D-serine+respiration
2521029042glucose 1-phosphate+respiration
2521032323glucuronamide+respiration
2521017754glycerol+respiration
2521017268myo-inositol+respiration
2521024996lactate+respiration
2521073784glycyl-l-glutamate+respiration
2521032032potassium gluconate+respiration
2521026271proline+respiration

antibiotic resistance

@refChEBImetaboliteis antibioticis resistant
2521028077rifampicinyesyes
252106472lincomycinyesyes
2521028001vancomycinyesyes
25210100147nalidixic acidyesyes
25210161680aztreonamyesyes

enzymes

@refvalueactivityec
25210acid phosphatase+3.1.3.2
25210alkaline phosphatase+3.1.3.1
25210alpha-chymotrypsin-3.4.21.1
25210alpha-fucosidase-3.2.1.51
25210alpha-galactosidase-3.2.1.22
25210alpha-glucosidase-3.2.1.20
25210alpha-mannosidase-3.2.1.24
25210beta-galactosidase-3.2.1.23
25210beta-glucosidase-3.2.1.21
25210beta-glucuronidase-3.2.1.31
25210catalase-1.11.1.6
25210cystine arylamidase-3.4.11.3
25210cytochrome oxidase+1.9.3.1
25210esterase (C 4)-
25210esterase Lipase (C 8)+
25210leucine arylamidase+3.4.11.1
25210lipase (C 14)-
25210N-acetyl-beta-glucosaminidase-3.2.1.52
25210naphthol-AS-BI-phosphohydrolase+
25210trypsin-3.4.21.4
25210urease-3.5.1.5
25210valine arylamidase-

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    25210C12:07.6
    25210C14:03.3
    25210C14:0 3OH / C16:1 iso I5.3
    25210C15:08
    25210C15:1ω6c4.3
    25210C16:029.3
    25210C16:1ω7c / C16:1ω6c21.1
    25210C17:02.4
    25210C17:0 cyclopropane14.2
    25210C18:00.7
  • type of FA analysis: whole cell analysis
  • incubation medium: Tryptic soy agar (TSA)
  • agar/liquid: agar
  • incubation temperature: 29
  • incubation time: 7
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

  • @ref: 25210
  • sample type: gut of the arboreal ant Cephalotes rohweri
  • sampling date: 2010
  • geographic location: Tucson, AZ
  • country: USA
  • origin.country: USA
  • continent: North America
  • latitude: 32
  • longitude: -111
  • enrichment culture: trypticase soy agar (TSA; Difco BD)
  • enrichment culture duration: 28 days
  • enrichment culture temperature: room temperature

isolation source categories

Cat1Cat2Cat3
#Host#Arthropoda#Insecta
#Host Body-Site#Gastrointestinal tract#Stomach

taxonmaps

  • @ref: 69479
  • File name: preview.99_19470.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_7;96_1447;97_11963;98_14711;99_19470&stattab=map
  • Last taxonomy: Saccharedens versatilis
  • 16S sequence: KT258895
  • Sequence Identity:
  • Total samples: 7
  • soil counts: 2
  • aquatic counts: 3
  • animal counts: 1
  • plant counts: 1

Sequence information

16S sequences

  • @ref: 25210
  • description: Saccharedens versatilis strain CAG32 16S ribosomal RNA gene, partial sequence
  • accession: KT258895
  • length: 1354
  • database: nuccore
  • NCBI tax ID: 1680761

GC content

  • @ref: 25210
  • GC-content: 58.6-59.8
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 25210

culture collection no.: NCIMB 15010, DSM 100909

literature

  • topic: Phylogeny
  • Pubmed-ID: 27902262
  • title: Saccharedens versatilis gen. nov., sp. nov., a sugar-degrading member of the Burkholderiales isolated from Cephalotes rohweri ant guts.
  • authors: Lin JY, Hobson WJ, Wertz JT
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001650
  • year: 2017
  • mesh: Alcaligenaceae/*classification/genetics/isolation & purification, Animals, Ants/*microbiology, Arizona, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Symbiosis, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25210J. Y. H. Lin, W. J.,Wertz, J. T.Saccharedens versatilis gen. nov., sp. nov., a sugar-degrading member of the Burkholderiales isolated from Cephalotes rohweri ant guts10.1099/ijsem.0.001650IJSEM 67: 447-453 201727902262
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/