Strain identifier

BacDive ID: 133349

Type strain: Yes

Species: Methylobacillus methanolivorans

Strain Designation: Z

Strain history: N. V. Doronina; G. K. Skryabin Inst. of Biochem. & Physiol. of Microorg., RAS, Russia; Z.

NCBI tax ID(s): 1848927 (species)

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General

@ref: 25194

BacDive-ID: 133349

keywords: 16S sequence, Bacteria, obligate aerobe, methylotroph, Gram-negative, motile, rod-shaped, colony-forming

description: Methylobacillus methanolivorans Z is an obligate aerobe, methylotroph, Gram-negative bacterium that forms circular colonies and was isolated from activated sludge of the drainage system.

NCBI tax id

  • NCBI tax id: 1848927
  • Matching level: species

strain history

  • @ref: 67770
  • history: N. V. Doronina; G. K. Skryabin Inst. of Biochem. & Physiol. of Microorg., RAS, Russia; Z.

doi: 10.13145/bacdive133349.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Nitrosomonadales
  • family: Methylophilaceae
  • genus: Methylobacillus
  • species: Methylobacillus methanolivorans
  • full scientific name: Methylobacillus methanolivorans Kaparullina et al. 2017

@ref: 25194

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Nitrosomonadales

family: Methylophilaceae

genus: Methylobacillus

species: Methylobacillus methanolivorans

strain designation: Z

type strain: yes

Morphology

cell morphology

  • @ref: 25194
  • gram stain: negative
  • cell length: 1.0-2.5 µm
  • cell width: 0.4-0.8 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: monotrichous, polar

colony morphology

@refcolony sizecolony colorcolony shapemedium usedincubation period
251941.0-2.0 mmwhite to slightly creamcircularmineral salts/methanol agar
635332 days

Culture and growth conditions

culture medium

@refnamegrowth
25194mineral salts/methanol agaryes
25194tryptic soy agar (TSA)no
25194LB mediano
25194nutrient agar (NA)no

culture temp

@refgrowthtypetemperaturerange
25194positivegrowth10.0-40.0
25194positiveoptimum29.0-35.0mesophilic
63533positivegrowth37mesophilic
67770positivegrowth29mesophilic

culture pH

@refabilitytypepH
25194positivegrowth6.0-8.0
25194positiveoptimum6.5-7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
25194obligate aerobe
63533aerobe

nutrition type

  • @ref: 25194
  • type: methylotroph

spore formation

  • @ref: 25194
  • spore formation: no

halophily

@refsalttested relationconcentrationgrowth
25194NaCloptimum0.05 %(w/v)
25194NaClgrowth1 %(w/v)no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2519416134ammonia+assimilation
2519428938ammonium+nitrogen source
2519417790methanol+oxidation
2519417632nitrate+reduction
2519417632nitrate+nitrogen source
2519427897tryptophan+energy source
2519416199urea+nitrogen source

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
2519417076streptomycinyesyes10 µg (disc)
2519448923erythromycinyesyes15 µg (disc)
2519428971ampicillinyesyes10 µg (disc)
251946472lincomycinyesyes2 µg (disc)
2519416869oleandomycinyesyes10 µg (disc)
251947507neomycinyesyes30 µg (disc)
251946104kanamycinyesyes30 µg (disc)
25194100147nalidixic acidyesyes30 µg (disc)
2519417833gentamicinyesyes10 µg (disc)
2519428368novobiocinyesyes5 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
2519415688acetoinno
2519416136hydrogen sulfideno
2519435581indoleyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
2519415688acetoin-
2519435581indole+

enzymes

@refvalueactivityec
25194catalase+1.11.1.6
25194cytochrome oxidase+1.9.3.1
25194glutamate dehydrogenase+1.4.1.4
25194methanol dehydrogenase+1.1.1.244
25194oxoglutarate dehydrogenase-1.2.4.2
25194Ribulose-bisphosphate carboxylase-4.1.1.39
25194tryptophan deaminase+4.1.99.1
25194urease+3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation proceduresampling date
25194activated sludge of the drainage systemBaikal paper mill (Irkutskaya oblast)RussiaRUSAsialiquid mineral medium â??Kâ??containing (g l-1): KH2PO4 â?? 2.0; (NH4)2SO4 â?? 2.0; MgSO47H2O â?? 0.025; NaCl â?? 0.5; FeSO47H2O â?? 0.002, pH 7.2 and 0.5 % (v/v) CH3OH7 days30incubation with shaking (180 r.p.m.)
63533Activated sludge,drainage system,Baikal papermillIrkutskaya oblast,SlyudyanskyiRussiaRUSAsia1976
67770Activated sludge of a drainage system of the Baikal paper millIrkutskaya OblastRussiaRUS

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Activated sludge

Sequence information

16S sequences

  • @ref: 25194
  • description: Methylobacillus methanolovorus strain Z 16S ribosomal RNA gene, partial sequence
  • accession: KX057479
  • length: 1443
  • database: nuccore
  • NCBI tax ID: 1848927

GC content

  • @ref: 67770
  • GC-content: 51
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 25194

culture collection no.: VKM B-3037, JCM 31401, CCUG 68999

straininfo link

  • @ref: 91416
  • straininfo: 410722

literature

  • topic: Phylogeny
  • Pubmed-ID: 27902271
  • title: Methylobacillus methanolivorans sp. nov., a novel non-pigmented obligately methylotrophic bacterium.
  • authors: Kaparullina EN, Trotsenko YA, Doronina NV
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001646
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Methanol/metabolism, Methylobacillus/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, Sewage/*microbiology, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25194E. N. T. Kaparullina, Y. A.,Doronina, N. V.Methylobacillus methanolivorans sp. nov., a novel non-pigmented obligately methylotrophic bacterium10.1099/ijsem.0.001646IJSEM 67: 245-431 201727902271
63533Curators of the CCUGhttps://www.ccug.se/strain?id=68999Culture Collection University of Gothenburg (CCUG) (CCUG 68999)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
91416Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID410722.1