Strain identifier

BacDive ID: 133346

Type strain: Yes

Species: Motilimonas eburnea

Strain Designation: YH6

NCBI tax ID(s): 1737488 (species)

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General

@ref: 25197

BacDive-ID: 133346

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, Gram-negative, motile, rod-shaped, colony-forming

description: Motilimonas eburnea YH6 is a facultative anaerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from marine sediment.

NCBI tax id

  • NCBI tax id: 1737488
  • Matching level: species

doi: 10.13145/bacdive133346.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Alteromonadales, not assigned to family
  • genus: Motilimonas
  • species: Motilimonas eburnea
  • full scientific name: Motilimonas eburnea Ling et al. 2017

@ref: 25197

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Alteromonadales, not assigned to family

genus: Motilimonas

species: Motilimonas eburnea

strain designation: YH6

type strain: yes

Morphology

cell morphology

  • @ref: 25197
  • gram stain: negative
  • cell length: 1.1-2.4 µm
  • cell width: 0.3-0.9 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: polar

colony morphology

  • @ref: 25197
  • colony size: 1 mm
  • colony color: ivory-white
  • colony shape: circular
  • incubation period: 3 days
  • medium used: 2216E agar

Culture and growth conditions

culture medium

  • @ref: 25197
  • name: 2216E agar
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
25197positivegrowth15.0-37.0
25197positiveoptimum28.0-33.0mesophilic

culture pH

@refabilitytypepHPH range
25197positivegrowth6.5-9.0alkaliphile
25197positiveoptimum7.5-8.5

Physiology and metabolism

oxygen tolerance

  • @ref: 25197
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 25197
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
25197NaClpositivegrowth1.0-8.0 %(w/v)
25197NaCloptimum2.0-4.0 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2519712936D-galactose-carbon source
2519712936D-galactose-builds acid from
2519717306maltose-carbon source
2519727082trehalose-builds acid from
2519727897tryptophan-energy source
25197581435-dehydro-D-gluconate+builds acid from
2519717925alpha-D-glucose+carbon source
2519728938ammonium+nitrogen source
2519716947citrate+carbon source
2519715824D-fructose+carbon source
2519715824D-fructose+builds acid from
2519717634D-glucose+builds acid from
2519716024D-mannose+carbon source
2519716024D-mannose+builds acid from
2519717596inosine+carbon source
25197506227N-acetylglucosamine+carbon source
25197506227N-acetylglucosamine+builds acid from

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
2519717076streptomycinyesyes10 µg
251976104kanamycinyesyes30 µg
2519727902tetracyclineyesyes30 µg
2519728001vancomycinyesyes30 µg
251976472lincomycinyesyes2 µg
2519753727sulfamethoxydiazineyesyes5 µg
2519731168acetylspiramycinyesyes30 µg
251973745clindamycinyesyes30 µg
25197100246norfloxacinyesyes30 µg
2519717698chloramphenicolyesyes30 µg
251977507neomycinyesyes30 µg
2519728971ampicillinyesyes10 µg
2519748923erythromycinyesyes15 µg
2519729007ceftriaxoneyesyes30 µg
25197204928cefotaximeyesyes30 µg
2519717334penicillinyesyes10 µg
2519728077rifampicinyesyes5 µg
2519728864tobramycinyesyes10 µg
251977731ofloxacinyesyes5 µg
25197100147nalidixic acidyesyes30 µg
2519717833gentamicinyesyes10 µg

metabolite production

@refChebi-IDmetaboliteproduction
2519715688acetoinno
2519735581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testcitrate test
2519715688acetoin-
2519735581indole-
2519716947citrate+

enzymes

@refvalueactivityec
25197acid phosphatase-3.1.3.2
25197agarase-3.2.1.81
25197alginase-3.2.1.16
25197alkaline phosphatase-3.1.3.1
25197alpha-chymotrypsin-3.4.21.1
25197alpha-fucosidase-3.2.1.51
25197alpha-galactosidase-3.2.1.22
25197alpha-glucosidase-3.2.1.20
25197alpha-mannosidase-3.2.1.24
25197amylase-
25197arginine dihydrolase+3.5.3.6
25197beta-galactosidase-3.2.1.23
25197beta-glucosidase-3.2.1.21
25197beta-glucuronidase-3.2.1.31
25197catalase-1.11.1.6
25197cellulase-3.2.1.4
25197cystine arylamidase-3.4.11.3
25197cytochrome oxidase+1.9.3.1
25197esterase-
25197esterase (C 4)-
25197esterase Lipase (C 8)+
25197gelatinase-
25197leucine arylamidase+3.4.11.1
25197lipase (C 14)-
25197N-acetyl-beta-glucosaminidase+3.2.1.52
25197naphthol-AS-BI-phosphohydrolase-
25197trypsin-3.4.21.4
25197tryptophan deaminase-4.1.99.1
25197urease-3.5.1.5
25197valine arylamidase-

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    25197C12:04.5
    25197C14:02.1
    25197C14:0 3OH / C16:1 iso3.7
    25197C15:02.6
    25197C16:021.5
    25197C16:1ω7c / C15:0 iso 2OH48.1
    25197C17:01.9
    25197C18:1ω7c7.9
    25197C18:1ω9c1.4
  • type of FA analysis: whole cell analysis
  • incubation medium: 2216E medium
  • agar/liquid: liquid
  • incubation temperature: 28
  • incubation time: 2
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA 40
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

  • @ref: 25197
  • sample type: marine sediment
  • sampling date: oktober 2017
  • geographic location: Weihai
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 36
  • longitude: 122
  • enrichment culture: 2216E agar (Hopebio)
  • enrichment culture duration: 4 days
  • enrichment culture temperature: 28
  • isolation procedure: diluted 10 000-fold with sterile seawater

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_130884.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_267;96_17644;97_21736;98_87594;99_130884&stattab=map
  • Last taxonomy: Motilimonas eburnea subclade
  • 16S sequence: KR610526
  • Sequence Identity:
  • Total samples: 2609
  • soil counts: 77
  • aquatic counts: 1055
  • animal counts: 1407
  • plant counts: 70

Sequence information

16S sequences

  • @ref: 25197
  • description: Gammaproteobacteria bacterium YH6 16S ribosomal RNA gene, partial sequence
  • accession: KR610526
  • length: 1467
  • database: nuccore
  • NCBI tax ID: 1737488

Genome sequences

  • @ref: 66792
  • description: Motilimonas eburnea YH6
  • accession: GCA_021295345
  • assembly level: scaffold
  • database: ncbi
  • NCBI tax ID: 1737488

GC content

  • @ref: 25197
  • GC-content: 46.5
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedyes94.02yes
gram-positiveno98.969yes
anaerobicno96.864yes
aerobicyes58.979yes
halophileyes51.332no
spore-formingno93.44yes
motileyes88.657no
glucose-fermentyes71.564no
thermophileno99.652yes
glucose-utilyes89.188yes

External links

@ref: 25197

culture collection no.: KCTC 42594, MCCC 1H00122

literature

  • topic: Phylogeny
  • Pubmed-ID: 27902247
  • title: Motilimonas eburnea gen. nov., sp. nov., isolated from coastal sediment.
  • authors: Ling SK, Guo LY, Chen GJ, Du ZJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001621
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gammaproteobacteria/*classification/genetics/isolation & purification, Geologic Sediments/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25197S. K. G. Ling, L. Y.,Chen, G. J.,Du, Z. J.Motilimonas eburnea gen. nov., sp. nov., isolated from coastal sediment10.1099/ijsem.0.001621IJSEM 67: 306-310 201727902247
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/