Strain identifier

BacDive ID: 133327

Type strain: Yes

Species: Krasilnikoviella muralis

Strain Designation: T6220-5-2b

Strain history: K. Okada and J. Sugiyama; Tokyo Natl. Res. Inst. for Cult. Prop. and TechnoSuruga Lab. Co., Ltd., Japan; T6220-5-2b.

NCBI tax ID(s): 1840606 (species)

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General

@ref: 66475

BacDive-ID: 133327

DSM-Number: 111259

keywords: 16S sequence, Bacteria, facultative anaerobe, Gram-positive

description: Krasilnikoviella muralis T6220-5-2b is a facultative anaerobe, Gram-positive bacterium that builds substrate mycelia and was isolated from surface of a mural painting on the west wall of the TT stone chamber interior.

NCBI tax id

  • NCBI tax id: 1840606
  • Matching level: species

strain history

@refhistory
66475<-JCM<-K. Okada and J. Sugiyama; Tokyo Natl. Res. Inst. for Cult. Prop. and TechnoSuruga Lab. Co., Ltd., Japan.
67770K. Okada and J. Sugiyama; Tokyo Natl. Res. Inst. for Cult. Prop. and TechnoSuruga Lab. Co., Ltd., Japan; T6220-5-2b.

doi: 10.13145/bacdive133327.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Promicromonosporaceae
  • genus: Krasilnikoviella
  • species: Krasilnikoviella muralis
  • full scientific name: Krasilnikoviella muralis Nishijima et al. 2017

@ref: 66475

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Micrococcales

family: Promicromonosporaceae

genus: Krasilnikoviella

species: Krasilnikoviella muralis

full scientific name: Krasilnikoviella muralis Nishijima et al. 2017

strain designation: T6220-5-2b

type strain: yes

Morphology

cell morphology

  • @ref: 25188
  • gram stain: positive

colony morphology

  • @ref: 25188
  • colony size: 1.0-2.0 mm
  • colony color: pale yellow
  • incubation period: 28 days
  • medium used: ISP 2

multicellular morphology

  • @ref: 25188
  • forms multicellular complex: yes
  • complex name: substrate mycelia
  • further description: non-motile, coccoid, V-shaped, Y-shaped and rod-like elements

pigmentation

  • @ref: 25188
  • production: no
  • name: melanin-like pigments

multimedia

  • @ref: 66475
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_111259.jpg
  • caption: Medium 535 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
25188ISP 2yes
25188ISP 3yes
25188ISP 4yes
25188ISP 5yes
25188Reasoner's 2A agar (R2A)yes
25188nutrient agar (NA)yes
25188SCD agaryes
66475TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
66475GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
66475RICH MEDIUM (DSMZ Medium 736)yeshttps://mediadive.dsmz.de/medium/736Name: RICH MEDIUM (DSMZ Medium 736) Composition: Agar 20.0 g/l Bacto peptone 10.0 g/l Yeast extract 5.0 g/l Malt extract 5.0 g/l Casamino acids 5.0 g/l Glycerol 2.0 g/l Meat extract 2.0 g/l MgSO4 x 7 H2O 1.0 g/l Tween 80 0.05 g/l Distilled water
66475R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
25188positivegrowth4.0-37.0
25188positiveoptimum25.0-30.0mesophilic
66475positivegrowth28mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
25188positivegrowth5.5-10.5alkaliphile
25188positiveoptimum7.5-8.5

Physiology and metabolism

oxygen tolerance

  • @ref: 25188
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 25188
  • spore formation: no

halophily

@refsalttested relationconcentration
25188NaClmaximum12 %(w/v)
25188NaCloptimum0.0-3.0 %(w/v)

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2), MK-9(H0), MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
25188168082-dehydro-D-gluconate-builds acid from
25188581435-dehydro-D-gluconate-builds acid from
2518817128adipate-assimilation
2518817108D-arabinose-builds acid from
2518815824D-fructose-builds acid from
2518828847D-fucose-builds acid from
2518862318D-lyxose-builds acid from
2518816899D-mannitol-builds acid from
2518816988D-ribose-builds acid from
2518817924D-sorbitol-builds acid from
2518816443D-tagatose-builds acid from
2518827689decanoate-assimilation
2518817113erythritol-builds acid from
2518816813galactitol-builds acid from
2518828066gentiobiose-builds acid from
2518824265gluconate-builds acid from
2518828087glycogen-builds acid from
2518817268myo-inositol-builds acid from
2518815443inulin-builds acid from
2518818403L-arabitol-builds acid from
2518818287L-fucose-builds acid from
2518817266L-sorbose-builds acid from
2518865328L-xylose-builds acid from
2518817716lactose-builds acid from
2518825115malate-assimilation
2518817306maltose-builds acid from
2518874863methyl beta-D-xylopyranoside-builds acid from
2518818401phenylacetate-assimilation
2518832032potassium gluconate-assimilation
2518853258sodium citrate-assimilation
2518827897tryptophan-energy source
2518817151xylitol-builds acid from
2518827613amygdalin+builds acid from
2518822599arabinose+assimilation
2518818305arbutin+builds acid from
25188casein+hydrolysis
2518817057cellobiose+builds acid from
2518815963ribitol+builds acid from
2518818333D-arabitol+builds acid from
2518812936D-galactose+builds acid from
2518817634D-glucose+builds acid from
2518816024D-mannose+builds acid from
2518865327D-xylose+builds acid from
2518816991dna+hydrolysis
251884853esculin+hydrolysis
2518817234glucose+fermentation
2518817234glucose+builds acid from
2518817234glucose+assimilation
2518817754glycerol+builds acid from
2518830849L-arabinose+builds acid from
2518817306maltose+assimilation
2518829864mannitol+assimilation
2518837684mannose+assimilation
251886731melezitose+builds acid from
2518828053melibiose+builds acid from
25188320061methyl alpha-D-glucopyranoside+builds acid from
2518843943methyl alpha-D-mannoside+builds acid from
25188506227N-acetylglucosamine+builds acid from
25188506227N-acetylglucosamine+assimilation
2518817632nitrate+reduction
2518816634raffinose+builds acid from
2518817814salicin+builds acid from
2518828017starch+hydrolysis
2518828017starch+builds acid from
2518827082trehalose+builds acid from
2518832528turanose+builds acid from
2518853426tween 80+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
2518816136hydrogen sulfideno
2518835581indoleno

metabolite tests

  • @ref: 25188
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
25188acid phosphatase-3.1.3.2
25188alkaline phosphatase+3.1.3.1
25188alpha-chymotrypsin-3.4.21.1
25188alpha-fucosidase-3.2.1.51
25188alpha-galactosidase-3.2.1.22
25188alpha-glucosidase+3.2.1.20
25188alpha-mannosidase+3.2.1.24
25188arginine dihydrolase-3.5.3.6
25188beta-galactosidase+3.2.1.23
25188beta-glucosidase+3.2.1.21
25188beta-glucuronidase-3.2.1.31
25188catalase+1.11.1.6
25188cystine arylamidase-3.4.11.3
25188cytochrome oxidase-1.9.3.1
25188esterase (C 4)+
25188esterase Lipase (C 8)+
25188lecithinase-
25188leucine arylamidase+3.4.11.1
25188lipase (C 14)-
25188N-acetyl-beta-glucosaminidase+3.2.1.52
25188naphthol-AS-BI-phosphohydrolase+
25188pyrazinamidase-3.5.1.B15
25188pyrrolidonyl arylamidase-3.4.19.3
25188trypsin-3.4.21.4
25188tryptophan deaminase-4.1.99.1
25188urease-3.5.1.5
25188valine arylamidase-

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    25188C15:0 anteiso50.7
    25188C17:0 anteiso1.9
    25188C14:02.9
    25188C16:03.1
    25188C14:0 iso2
    25188C15:0 iso34.2
    25188C16:0 iso5.3
  • type of FA analysis: whole cell analysis
  • incubation medium: SCD
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 3
  • software version: Sherlock 6.0
  • library/peak naming table: TSBA 6
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedurehost species
25188surface of a mural painting (the white tiger, Byakko) on the west wall of the TT stone chamber interiorAsuka village, Nara PrefectureJapanJPNAsianutrient agar (CM3; Oxoid)3 days30under aerobic conditions
66475mouldy spots on the surface of a mural painting (the white tiger, Byakko) inside the stone chamber of the Takamatsuzuka.Asuka Village, Nara Pref., JapanJapanJPNAsia
67770A mouldy spot on the surface of a mural painting (the white tiger, Byakko) inside the stone chamber of the Takamatsuzuka TumulusAsuka Village, Nara Pref.JapanJPNAsiaByakko

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Other
  • Cat3: #Painting

taxonmaps

  • @ref: 69479
  • File name: preview.99_7502.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_179;97_4293;98_5496;99_7502&stattab=map
  • Last taxonomy: Krasilnikoviella
  • 16S sequence: LC148843
  • Sequence Identity:
  • Total samples: 139
  • soil counts: 61
  • aquatic counts: 11
  • animal counts: 20
  • plant counts: 47

Safety information

risk assessment

  • @ref: 66475
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 25188
  • description: Krasilnikoviella muralis gene for 16S ribosomal RNA, partial cds, strain: T6220-5-2b
  • accession: LC148843
  • length: 1447
  • database: nuccore
  • NCBI tax ID: 1840606

GC content

@refGC-contentmethod
2518875.2genome sequence analysis
6647575.2high performance liquid chromatography (HPLC)

External links

@ref: 66475

culture collection no.: JCM 28789, NCIMB 15040, DSM 111259

straininfo link

  • @ref: 91411
  • straininfo: 406028

literature

  • topic: Phylogeny
  • Pubmed-ID: 27902237
  • title: Krasilnikoviella muralis gen. nov., sp. nov., a member of the family Promicromonosporaceae, isolated from the Takamatsuzuka Tumulus stone chamber interior and reclassification of Promicromonospora flava as Krasilnikoviella flava comb. nov.
  • authors: Nishijima M, Tazato N, Handa Y, Umekawa N, Kigawa R, Sano C, Sugiyama J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001618
  • year: 2017
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Japan, Nucleic Acid Hybridization, *Paintings, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25188M. T. Nishijima, N.,Handa, Y.,Umekawa, N.,Kigawa, R.,Sano, C.,Sugiyama, J.Krasilnikoviella muralis gen. nov., sp. nov., a member of the family Promicromonosporaceae, isolated from the Takamatsuzuka Tumulus stone chamber interior and reclassification of Promicromonospora flava as Krasilnikoviella flava comb. nov10.1099/ijsem.0.001618IJSEM 67: 294-300 201727902237
66475Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-111259Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 111259)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
91411Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID406028.1