Strain identifier

BacDive ID: 133321

Type strain: Yes

Species: Marimonas arenosa

Strain history: <- Y. Muramatsu, NBRC; NBRC 111988 <- W. Kim, Chung-Ang Univ., Seoul, Republic of Korea; CAU 1311

NCBI tax ID(s): 1795305 (species)

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General

@ref: 64839

BacDive-ID: 133321

DSM-Number: 108276

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Marimonas arenosa CAU 1311 is an obligate aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from sea sand sample.

NCBI tax id

  • NCBI tax id: 1795305
  • Matching level: species

strain history

  • @ref: 64839
  • history: <- Y. Muramatsu, NBRC; NBRC 111988 <- W. Kim, Chung-Ang Univ., Seoul, Republic of Korea; CAU 1311

doi: 10.13145/bacdive133321.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Roseobacteraceae
  • genus: Marimonas
  • species: Marimonas arenosa
  • full scientific name: Marimonas arenosa Thongphrom et al. 2017

@ref: 64839

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Marimonas

species: Marimonas arenosa

full scientific name: Marimonas arenosa Thongphrom et al. 2017

type strain: yes

Morphology

cell morphology

  • @ref: 25155
  • gram stain: negative
  • cell length: 1.4-2.0 µm
  • cell width: 0.3-0.6 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 25155
  • colony color: beige
  • colony shape: circular
  • incubation period: 3 days
  • medium used: marine agar (MA)

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
25155marine agar (MA)yes
64839BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
25155positiveoptimum30mesophilic
25155positivegrowth20.0-37.0
64839positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
25155positiveoptimum7.5
25155positivegrowth6.5-10.0alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 25155
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 25155
  • spore formation: no

halophily

@refsalttested relationconcentrationgrowth
25155NaCloptimum2 %(w/v)
25155NaClgrowth0.0-6.0 %(w/v)positive

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
25155casein-hydrolysis
25155citrate-hydrolysis16947
25155esculin-hydrolysis4853
25155glucose-carbon source17234
25155maltose-carbon source17306
25155mannose-carbon source37684
25155N-acetylglucosamine-carbon source506227
25155nitrate-reduction17632
25155starch-hydrolysis28017
25155tryptophan-energy source27897
251555-dehydro-D-gluconate+carbon source58143
25155gelatin+hydrolysis5291
25155L-tryptophan+assimilation16828
68368nitrate-reduction17632
68368L-arabinose+fermentation30849
68368amygdalin+fermentation27613
68368melibiose+fermentation28053
68368sucrose+fermentation17992
68368L-rhamnose+fermentation62345
68368sorbitol+fermentation30911
68368myo-inositol+fermentation17268
68368D-mannitol+fermentation16899
68368D-glucose-fermentation17634
68368gelatin+hydrolysis5291
68368urea-hydrolysis16199
68368citrate-assimilation16947
68368ornithine-degradation18257
68368lysine-degradation25094
68368arginine-hydrolysis29016

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.group IDis resistantresistance conc.
25155124991cefalotinyesyes30 µg (disc)
2515517076streptomycinyesyes10 µg (disc)
25155209807cefoxitinyesyes30 µg (disc)
2515527902tetracyclineyesyes30 µg (disc)
2515528864tobramycinyesyes10 µg (disc)
2515517833gentamicinyesyes10 µg (disc)
2515545924trimethoprimyes26yes1.25 µg (disc)
251559332sulfamethoxazoleyes26yes23.75 µg (disc)
25155100147nalidixic acidyesyes30 µg (disc)
2515517334penicillinyesyes10 Unit
2515528971ampicillinyesyes10 µg (disc)
251558309polymyxin byesyes300 Unit

metabolite production

@refChebi-IDmetaboliteproduction
2515515688acetoinno
2515516136hydrogen sulfideno
2515535581indoleno
6836817997dinitrogenno
6836816301nitriteno
6836815688acetoinyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
2515515688acetoin-
2515535581indole-
6836815688acetoin+

enzymes

@refvalueactivityec
25155acid phosphatase-3.1.3.2
25155alkaline phosphatase-3.1.3.1
25155alpha-chymotrypsin-3.4.21.1
25155alpha-fucosidase-3.2.1.51
25155alpha-galactosidase-3.2.1.22
25155alpha-glucosidase-3.2.1.20
25155alpha-mannosidase-3.2.1.24
25155arginine dihydrolase-3.5.3.6
25155beta-galactosidase-3.2.1.23
25155beta-glucosidase-3.2.1.21
25155beta-glucuronidase-3.2.1.31
25155catalase+1.11.1.6
25155cystine arylamidase-3.4.11.3
25155cytochrome oxidase+1.9.3.1
25155esterase (C 4)+
25155esterase Lipase (C 8)+
25155leucine arylamidase+3.4.11.1
25155lipase (C 14)-
25155lysine decarboxylase-4.1.1.18
25155N-acetyl-beta-glucosaminidase-3.2.1.52
25155naphthol-AS-BI-phosphohydrolase+
25155ornithine decarboxylase-4.1.1.17
25155trypsin-3.4.21.4
25155tryptophan deaminase-4.1.99.1
25155urease-3.5.1.5
25155valine arylamidase+
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)-
68382lipase (C 14)-
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    2515511-Methyl C18:1ω7c6.57
    25155C12:0 3OH3.47
    25155C16:02.45
    25155C18:02.79
    25155C18:1ω7c84.16
  • type of FA analysis: whole cell analysis
  • incubation medium: Marine Agar (MA)
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 3
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA 5
  • system: MIS MIDI
  • cutoff value:

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
64839---+/--+/---------------

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpVPGELGLUMANINOSorRHASACMELAMYARANO2N2
64839--------++-++++++++--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
25155sea sand sampleEurwangri beach in IncheonRepublic of KoreaKORAsiamarine agar (MA; Difco)7 days30standard dilution plating technique according to Gordon and Mihm
64839Sea sandIncheon, Eurwangri beachRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Terrestrial#Sandy

Safety information

risk assessment

  • @ref: 64839
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 25155
  • description: Marivita sp. CAU 1311 16S ribosomal RNA gene, partial sequence
  • accession: KU671052
  • length: 1437
  • database: nuccore
  • NCBI tax ID: 1795305

GC content

  • @ref: 25155
  • GC-content: 60.74
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 64839

culture collection no.: CAU 1311, KCTC 52189, NBRC 111988, DSM 108276

straininfo link

  • @ref: 91410
  • straininfo: 397935

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27902202Marimonas arenosa gen. nov., sp. nov., isolated from sea sand.Thongphrom C, Kim JH, Yoon JH, Bora N, Kim WInt J Syst Evol Microbiol10.1099/ijsem.0.0015812017Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Silicon Dioxide, Ubiquinone/analogs & derivatives/chemistryTranscriptome
Phylogeny29160201Aquicoccus porphyridii gen. nov., sp. nov., isolated from a small marine red alga, Porphyridium marinum.Feng T, Kim KH, Jeong SE, Kim W, Jeon COInt J Syst Evol Microbiol10.1099/ijsem.0.0024982017Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, Porphyridium/*microbiology, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodobacteraceae/*classification/genetics/isolation & purification, Sequence Analysis, DNA, Ubiquinone/chemistryTranscriptome
Phylogeny31639073Marimonas lutisalis sp. nov., isolated from a tidal mudflat and emended description of the genus Marimonas.Lee SD, Jeon D, Kim YJ, Kim IS, Choe H, Kim JSInt J Syst Evol Microbiol10.1099/ijsem.0.0037492020Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Islands, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodobacteraceae/*classification/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25155C. K. Thongphrom, J. H.,Yoon, J. H.,Bora, N.,Kim, W.Marimonas arenosa gen. nov., sp. nov., isolated from sea sand10.1099/ijsem.0.001581IJSEM 67: 121-126 201727902202
64839Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-108276Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 108276)
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
91410Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397935.1