Strain identifier

BacDive ID: 133315

Type strain: Yes

Species: Soortia roseihalophila

Strain Designation: Bsw-2b

NCBI tax ID(s): 1889947 (species)

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General

@ref: 25161

BacDive-ID: 133315

keywords: 16S sequence, Bacteria, aerobe, slightly halophilic, heterotroph, mesophilic, colony-forming, pigmented

description: Soortia roseihalophila Bsw-2b is an aerobe, slightly halophilic, heterotroph bacterium that forms circular colonies and has a orange pigmentation.

NCBI tax id

  • NCBI tax id: 1889947
  • Matching level: species

doi: 10.13145/bacdive133315.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/balneolota
  • domain: Bacteria
  • phylum: Balneolota
  • class: Balneolia
  • order: Balneolales
  • family: Soortiaceae
  • genus: Soortia
  • species: Soortia roseihalophila
  • full scientific name: Soortia roseihalophila Amoozegar et al. 2017

@ref: 25161

domain: Bacteria

phylum: Balneolaeota

class: Balneolia

order: Balneolales

family: Soortiaceae

genus: Soortia

species: Soortia roseihalophila

strain designation: Bsw-2b

type strain: yes

Morphology

colony morphology

  • @ref: 25161
  • colony size: 1.0-2.0 mm
  • colony color: orange
  • colony shape: circular
  • incubation period: 1 day
  • medium used: MNA (modified nutrient agar)

pigmentation

  • @ref: 25161
  • production: yes
  • color: orange
  • name: Flexirubin

Culture and growth conditions

culture medium

  • @ref: 25161
  • name: MNA (modified nutrient agar)
  • growth: yes
  • composition: NaCl, 20.25; peptone, 5.0; yeast extract, 2.0; meat extract, 1.0; KCl, 0.5; MgSO4 * 7H2O, 2.4; CaCl2 * 2H2O, 0.007; MgCl2 * 6H2O, 1.6; NaHCO3 , 0.0045 and agar, 15.0; distilled water, 1000 ml. pH 7.3

culture temp

@refgrowthtypetemperaturerange
25161positivegrowth28-34mesophilic
25161positiveoptimum30mesophilic

culture pH

@refabilitytypepH
25161positivegrowth6.0-8.0
25161positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 25161
  • oxygen tolerance: aerobe

nutrition type

  • @ref: 25161
  • type: heterotroph

spore formation

  • @ref: 25161
  • type of spore: endospore
  • spore formation: no

halophily

@refhalophily levelsaltgrowthtested relationconcentration
25161slightly halophilicNaClpositivegrowth1-3 %
25161NaCloptimum2 %
25161NaClnogrowth0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2516130089acetate-carbon source
2516117128adipate-carbon source
2516115963ribitol-carbon source
2516127613amygdalin-builds acid from
25161casein-hydrolysis
2516116947citrate-carbon source
2516117634D-glucose-builds acid from
2516116899D-mannitol-builds acid from
2516116899D-mannitol-carbon source
2516117924D-sorbitol-builds acid from
2516127689decanoate-carbon source
2516116991dna-hydrolysis
2516117234glucose-fermentation
2516117268myo-inositol-builds acid from
2516162345L-rhamnose-builds acid from
2516125115malate-carbon source
2516128053melibiose-builds acid from
2516117632nitrate-reduction
2516126546rhamnose-carbon source
2516130031succinate-carbon source
2516117992sucrose-builds acid from
2516116199urea-hydrolysis
2516122599arabinose+builds acid from
2516117057cellobiose+carbon source
2516112936D-galactose+carbon source
2516117634D-glucose+carbon source
251614853esculin+hydrolysis
251615291gelatin+hydrolysis
2516124265gluconate+carbon source
2516117754glycerol+carbon source
2516117268myo-inositol+carbon source
2516130849L-arabinose+carbon source
2516117306maltose+carbon source
2516137684mannose+carbon source
2516130911sorbitol+carbon source
2516128017starch+hydrolysis
2516153424tween 20+hydrolysis
2516153423tween 40+hydrolysis
2516153425tween 60+hydrolysis
2516153426tween 80+hydrolysis
2516118222xylose+carbon source

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
2516117698chloramphenicolyesyes30 µg
2516127902tetracyclineyesyes30 µg
251612637amikacinyesyes30 µg
2516128077rifampicinyesyes5 µg
25161209807cefoxitinyesyes30 µg
251618309polymyxin byesyes300 Unit
2516148923erythromycinyesyes5 µg
2516117833gentamicinyesyes10 µg
2516128669bacitracinyesyes10 µg
2516118208penicillin gyesyes10 Unit
2516128971ampicillinyesyes10 µg
25161100147nalidixic acidyesyes30 µg
2516128864tobramycinyesyes10 µg
251616104kanamycinyesyes5 µg

metabolite production

@refChebi-IDmetaboliteproduction
2516115688acetoinno
2516116136hydrogen sulfideno
2516135581indoleno

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-test
2516117234glucose-
2516115688acetoin-

enzymes

@refvalueactivityec
25161acid phosphatase+3.1.3.2
25161alkaline phosphatase+3.1.3.1
25161alpha-chymotrypsin+3.4.21.1
25161alpha-fucosidase-3.2.1.51
25161alpha-galactosidase-3.2.1.22
25161alpha-glucosidase-3.2.1.20
25161alpha-mannosidase-3.2.1.24
25161arginine dihydrolase-3.5.3.6
25161beta-galactosidase+3.2.1.23
25161beta-glucosidase+3.2.1.21
25161beta-glucuronidase+3.2.1.31
25161catalase-1.11.1.6
25161cystine arylamidase+3.4.11.3
25161cytochrome oxidase+1.9.3.1
25161esterase (C 4)-
25161esterase Lipase (C 8)-
25161leucine arylamidase-3.4.11.1
25161lipase (C 14)-
25161lysine decarboxylase+4.1.1.18
25161N-acetyl-beta-glucosaminidase-3.2.1.52
25161naphthol-AS-BI-phosphohydrolase+
25161ornithine decarboxylase-4.1.1.17
25161trypsin+3.4.21.4
25161tryptophan deaminase-4.1.99.1
25161valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    25161C15:0 anteiso1.2
    25161C17:0 anteiso16.1
    25161C16:01.1
    25161C16:1ω7c / C15:0 iso 2OH11.4
    25161C16:1ω9c1.1
    25161C13:0 iso17.4
    25161C14:0 iso13.5
    25161C15:0 iso28.2
    25161C15:1 iso3.9
    25161C16:0 iso2.2
    25161C17:1 iso ω9c1.8
  • type of FA analysis: whole cell analysis
  • incubation medium: MNA with 2% NaCl
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation pH: 7.00
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA 40
  • system: MIS MIDI
  • method/protocol: Kämpfer & Kroppenstedt 1996
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 25161
  • sample type: water sample from Badab-Soort travertine spring in North of Iran
  • geographic location: Badab-Soort travertine spring in North of Iran
  • country: Iran
  • origin.country: IRN
  • continent: Asia
  • latitude: 36.354
  • longitude: 53.856

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Spring

taxonmaps

  • @ref: 69479
  • File name: preview.99_143436.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_7176;96_31502;97_39852;98_51872;99_143436&stattab=map
  • Last taxonomy: Soortia roseihalophila subclade
  • 16S sequence: KU695464
  • Sequence Identity:
  • Total samples: 213
  • soil counts: 33
  • aquatic counts: 168
  • animal counts: 11
  • plant counts: 1

Sequence information

16S sequences

  • @ref: 25161
  • description: 16S rRNA gene sequence
  • accession: KU695464
  • length: 1516
  • database: ena
  • NCBI tax ID: 1889947

GC content

  • @ref: 25161
  • GC-content: 40.5
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 25161

culture collection no.: IBRC-M10915, LMG 28547

literature

  • topic: Phylogeny
  • Pubmed-ID: 27902267
  • title: Soortia roseihalophila gen. nov., sp. nov., a new taxon in the order Balneolales isolated from a travertine spring, and description of Soortiaceae fam. nov.
  • authors: Amoozegar MA, Khansha J, Mehrshad M, Shahzadeh Fazeli SA, Ramezani M, de la Haba RR, Sanchez-Porro C, Ventosa A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001583
  • year: 2017
  • mesh: Bacterial Typing Techniques, Bacteroidetes/*classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Iran, Natural Springs/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25161M. A. K. Amoozegar, J.,Mehrshad, M.,Shahzadeh Fazeli, S. A.,Ramezani, M.,de la Haba, R. R.,Sánchez-Porro, C.,Ventosa, A.Soortia roseihalophila gen. nov., sp. nov., a new taxon in the order Balneolales isolated from a travertine spring, and description of Soortiaceae fam. nov10.1099/ijsem.0.001583IJSEM 67: 113-120 201727902267
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/