Strain identifier

BacDive ID: 133291

Type strain: Yes

Species: Sphingomonas piscinae

Strain Designation: LYH-20

NCBI tax ID(s): 1664572 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 8.1 (current version)

General

@ref: 25088

BacDive-ID: 133291

keywords: 16S sequence, Bacteria, aerobe, chemoorganotroph, Gram-negative, filament-shaped, colony-forming

description: Sphingomonas piscinae LYH-20 is an aerobe, chemoorganotroph, Gram-negative bacterium that forms circular colonies and was isolated from fish culture pond.

NCBI tax id

  • NCBI tax id: 1664572
  • Matching level: species

doi: 10.13145/bacdive133291.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Sphingomonadales
  • family: Sphingomonadaceae
  • genus: Sphingomonas
  • species: Sphingomonas piscinae
  • full scientific name: Sphingomonas piscinae Chen et al. 2016

@ref: 25088

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Sphingomonadales

family: Sphingomonadaceae

genus: Sphingomonas

species: Sphingomonas piscinae

strain designation: LYH-20

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
25088negative0.6-2.0 µm0.2-0.4 µmfilament-shapedno
25088rod-shaped

colony morphology

  • @ref: 25088
  • colony size: 0.5-1.7 mm
  • colony color: yellow
  • colony shape: circular
  • incubation period: 3 days
  • medium used: R2A agar (Reasoner's 2A agar)

Culture and growth conditions

culture medium

@refnamegrowth
25088NA (Nutrient Agar)yes
25088Reasoner's 2A agar (R2A)yes

culture temp

@refgrowthtypetemperaturerange
25088positivegrowth15.0-40.0
25088positiveoptimum30mesophilic

culture pH

@refabilitytypepHPH range
25088positivegrowth5.0-9.0alkaliphile
25088positiveoptimum8.0-9.0

Physiology and metabolism

oxygen tolerance

  • @ref: 25088
  • oxygen tolerance: aerobe

nutrition type

  • @ref: 25088
  • type: chemoorganotroph

spore formation

  • @ref: 25088
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
25088NaClpositivegrowth0.0-1.0 %
25088NaCloptimum0.0-0.1 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2508817128adipate-carbon source
2508822599arabinose-carbon source
2508818305arbutin-builds acid from
2508885146carboxymethylcellulose-hydrolysis
25088casein-hydrolysis
2508817057cellobiose-builds acid from
2508817029chitin-hydrolysis
2508816947citrate-carbon source
25088corn oil-hydrolysis
2508815824D-fructose-builds acid from
2508828847D-fucose-builds acid from
2508812936D-galactose-builds acid from
2508817634D-glucose-builds acid from
2508862318D-lyxose-builds acid from
2508816024D-mannose-builds acid from
2508865327D-xylose-builds acid from
2508816991dna-hydrolysis
250884853esculin-hydrolysis
250885291gelatin-hydrolysis
2508828066gentiobiose-builds acid from
2508824265gluconate-carbon source
2508817234glucose-fermentation
2508830849L-arabinose-builds acid from
2508818287L-fucose-builds acid from
2508825115malate-carbon source
2508817306maltose-carbon source
2508829864mannitol-carbon source
2508837684mannose-carbon source
2508874863methyl beta-D-xylopyranoside-builds acid from
2508837657methyl D-glucoside-builds acid from
25088506227N-acetylglucosamine-builds acid from
25088506227N-acetylglucosamine-carbon source
2508817632nitrate-reduction
2508816301nitrite-reduction
2508816634raffinose-builds acid from
2508817814salicin-builds acid from
2508828017starch-hydrolysis
2508817992sucrose-builds acid from
2508827082trehalose-builds acid from
2508827897tryptophan-energy source
2508832528turanose-builds acid from
2508853424tween 20-hydrolysis
2508853426tween 80-hydrolysis
2508816199urea-hydrolysis
25088168082-dehydro-D-gluconate+builds acid from
25088581435-dehydro-D-gluconate+builds acid from
2508816947citrate+carbon source
2508816988D-ribose+builds acid from
2508816443D-tagatose+builds acid from
250884853esculin+builds acid from
2508817234glucose+carbon source
2508817306maltose+builds acid from
2508828017starch+builds acid from
2508853423tween 40+hydrolysis
2508853425tween 60+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.group IDis sensitivesensitivity conc.
2508817076streptomycinyesyes10 µg
2508845924trimethoprimyesyes1.25 µg25
25088102130sulfamerazineyesyes23.75 µg25
2508828077rifampicinyesyes5 µg
2508817698chloramphenicolyesyes30 µg
2508817833gentamicinyesyes10 µg
250886104kanamycinyesyes30 µg
2508827902tetracyclineyesyes30 µg
2508828368novobiocinyesyes30 µg
2508828971ampicillinyesyes10 µg
25088100147nalidixic acidyesyes30 µg
2508818208penicillin gyesyes10 Unit

metabolite production

@refChebi-IDmetaboliteproduction
2508815688acetoinno
2508835581indoleno

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-testindole testcitrate test
2508817234glucose-
2508815688acetoin-
2508835581indole-
2508816947citrate-

enzymes

@refvalueactivityec
25088acid phosphatase+3.1.3.2
25088alkaline phosphatase+3.1.3.1
25088alpha-chymotrypsin+3.4.21.1
25088alpha-fucosidase-3.2.1.51
25088alpha-galactosidase+3.2.1.22
25088alpha-glucosidase+3.2.1.20
25088alpha-mannosidase-3.2.1.24
25088arginine dihydrolase-3.5.3.6
25088beta-galactosidase+3.2.1.23
25088beta-glucosidase-3.2.1.21
25088beta-glucuronidase-3.2.1.31
25088catalase+1.11.1.6
25088cystine arylamidase+3.4.11.3
25088cytochrome oxidase+1.9.3.1
25088esterase (C 4)+
25088esterase Lipase (C 8)+
25088leucine arylamidase+3.4.11.1
25088lipase (C 14)+
25088N-acetyl-beta-glucosaminidase+3.2.1.52
25088naphthol-AS-BI-phosphohydrolase+
25088trypsin+3.4.21.4
25088tryptophan deaminase-4.1.99.1
25088urease-3.5.1.5
25088valine arylamidase+

Isolation, sampling and environmental information

isolation

  • @ref: 25088
  • sample type: fish culture pond
  • geographic location: Sanyi Township in the vicinity of MiaoLi County
  • country: Taiwan
  • origin.country: TWN
  • continent: Asia
  • latitude: 24.388
  • longitude: 120.769

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Aquaculture
#Environmental#Aquatic#Pond (small)
#Host#Fishes

Sequence information

16S sequences

  • @ref: 25088
  • description: Sphingomonas sp. LYH-20 partial 16S rRNA gene, strain LYH-20
  • accession: LN864675
  • length: 1417
  • database: nuccore
  • NCBI tax ID: 1664572

GC content

  • @ref: 25088
  • GC-content: 67.5
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 25088

culture collection no.: BCRC 80911, LMG 29002, KCTC 42741

literature

  • topic: Phylogeny
  • Pubmed-ID: 27654878
  • title: Sphingomonas piscinae sp. nov., isolated from a fish pond.
  • authors: Chen WM, Li YS, Sheu SY
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001511
  • year: 2016
  • mesh: Animals, Aquaculture, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fishes, Hydroxybutyrates/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Polyamines/chemistry, Polyesters/chemistry, Ponds/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sphingomonas/*classification/genetics/isolation & purification, Taiwan, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25088W. M. L. Chen, Y. S.,Sheu, S. Y.Sphingomonas piscinae sp. nov., isolated from a fish pond10.1099/ijsem.0.001511IJSEM 66: 5301-5308 201627654878