Strain identifier

BacDive ID: 13323

Type strain: Yes

Species: Prauserella marina

Strain Designation: MS 498

Strain history: DSM 45268 <-- S.-K. Tang MS498 <-- J. Bian et al..

NCBI tax ID(s): 530584 (species)

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General

@ref: 16076

BacDive-ID: 13323

DSM-Number: 45268

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Prauserella marina MS 498 is an aerobe, spore-forming, mesophilic bacterium that was isolated from ocean sediment.

NCBI tax id

  • NCBI tax id: 530584
  • Matching level: species

strain history

@refhistory
16076<- S.-K. Tang, YIM; MS 498 <- J. Bian and et al.
67770DSM 45268 <-- S.-K. Tang MS498 <-- J. Bian et al..

doi: 10.13145/bacdive13323.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Prauserella
  • species: Prauserella marina
  • full scientific name: Prauserella marina Wang et al. 2010

@ref: 16076

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Prauserella

species: Prauserella marina

full scientific name: Prauserella marina Wang et al. 2010

strain designation: MS 498

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
29473positiveno
69480no93.593
69480positive100

pigmentation

  • @ref: 29473
  • production: yes

multimedia

  • @ref: 16076
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45268.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16076GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
16076GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
16076positivegrowth28mesophilic
29473positivegrowth15-45
29473positiveoptimum32.5mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
29473positivegrowth06-09alkaliphile
29473positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 29473
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481yes94
69480yes99.448

halophily

  • @ref: 29473
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4), MK-8(H4), MK-9(H6), MK-10(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2947322599arabinose+carbon source
2947328260galactose+carbon source
2947317234glucose+carbon source
2947317306maltose+carbon source
2947329864mannitol+carbon source
2947337684mannose+carbon source
2947326546rhamnose+carbon source
2947333942ribose+carbon source
2947327082trehalose+carbon source
2947318222xylose+carbon source

enzymes

@refvalueactivityec
29473catalase+1.11.1.6
29473gelatinase+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
16076ocean sedimentNanhaiChinaCHNAsia
67770Ocean sediment from the South China Sea

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_104168.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_7302;97_8756;98_70969;99_104168&stattab=map
  • Last taxonomy: Prauserella
  • 16S sequence: FJ444996
  • Sequence Identity:
  • Total samples: 162
  • soil counts: 127
  • aquatic counts: 3
  • animal counts: 20
  • plant counts: 12

Safety information

risk assessment

  • @ref: 16076
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16076
  • description: Prauserella marina strain MS498 16S ribosomal RNA gene, partial sequence
  • accession: FJ444996
  • length: 1443
  • database: ena
  • NCBI tax ID: 530584

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Prauserella marina DSM 45268GCA_002240355completencbi530584
66792Prauserella marina strain CGMCC 4.5506530584.3wgspatric530584
66792Prauserella marina strain DSM 45268530584.4completepatric530584
66792Prauserella marina strain DSM 45268530584.6plasmidpatric530584
66792Prauserella marina strain DSM 45268530584.7wgspatric530584
66792Prauserella marina DSM 452682770939488draftimg530584
66792Prauserella marina CGMCC 4.55062675903049draftimg530584
67770Prauserella marina CGMCC 4.5506GCA_900102755scaffoldncbi530584
67770Prauserella marina DSM 45268GCA_003182115scaffoldncbi530584

GC content

@refGC-contentmethod
1607666.1
6777066.1high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes94no
flagellatedno97.692no
gram-positiveyes91.749yes
anaerobicno99.354yes
aerobicyes91.674yes
halophileyes61.733no
spore-formingyes80.312no
glucose-utilyes89.17no
thermophileno96.678yes
motileno92.224no
glucose-fermentno92.245no

External links

@ref: 16076

culture collection no.: DSM 45268, CCTCC AA 208056, JCM 18301, CGMCC 4.5506, NBRC 105212

straininfo link

  • @ref: 82523
  • straininfo: 407584

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19666793Prauserella marina sp. nov., isolated from ocean sediment of the South China Sea.Wang J, Li Y, Bian J, Tang SK, Ren B, Chen M, Li WJ, Zhang LXInt J Syst Evol Microbiol10.1099/ijs.0.013862-02009Actinomycetales/chemistry/*classification/genetics/*isolation & purification, Bacterial Typing Techniques, China, DNA, Ribosomal/analysis/genetics, Genes, rRNA, Genotype, Geologic Sediments/*microbiology, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Species SpecificityGenetics
Phylogeny25832628Prauserella endophytica sp. nov., an endophytic actinobacterium isolated from Tamarix taklamakanensis.Liu JM, Habden X, Guo L, Tuo L, Jiang ZK, Liu SW, Liu XF, Chen L, Li RF, Zhang YQ, Sun CHAntonie Van Leeuwenhoek10.1007/s10482-015-0435-12015Actinobacteria/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Endophytes/*classification/genetics/*isolation & purification/physiology, Fatty Acids/analysis, Glycolipids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Tamaricaceae/*microbiologyEnzymology
Phylogeny28857021Prauserella oleivorans sp. nov., a halophilic and thermotolerant crude-oil-degrading actinobacterium isolated from an oil-contaminated mud pit.Dastgheib SMM, Tirandaz H, Moshtaghi Nikou M, Ramezani M, Shavandi M, Amoozegar MA, Ventosa AInt J Syst Evol Microbiol10.1099/ijsem.0.0021242017Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Iran, Nucleic Acid Hybridization, Oil and Gas Fields/*microbiology, Peptidoglycan/chemistry, Petroleum/*microbiology, Petroleum Pollution, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Soil Pollutants, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
16076Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45268)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45268
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29473Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2587528776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
82523Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID407584.1StrainInfo: A central database for resolving microbial strain identifiers