Strain identifier

BacDive ID: 133195

Type strain: Yes

Species: Mucilaginibacter puniceus

Strain Designation: WS 71

Strain history: C. N. Seong; Sunchon Natl. Univ., South Korea; WS71.

NCBI tax ID(s): 1196020 (species)

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General

@ref: 24991

BacDive-ID: 133195

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming

description: Mucilaginibacter puniceus WS 71 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from freshwater.

NCBI tax id

  • NCBI tax id: 1196020
  • Matching level: species

strain history

  • @ref: 67770
  • history: C. N. Seong; Sunchon Natl. Univ., South Korea; WS71.

doi: 10.13145/bacdive133195.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Sphingobacteriia
  • order: Sphingobacteriales
  • family: Sphingobacteriaceae
  • genus: Mucilaginibacter
  • species: Mucilaginibacter puniceus
  • full scientific name: Mucilaginibacter puniceus Lee et al. 2016

@ref: 24991

domain: Bacteria

phylum: Bacteroidetes

class: Sphingobacteriia

order: Sphingobacteriales

family: Sphingobacteriaceae

genus: Mucilaginibacter

species: Mucilaginibacter puniceus

strain designation: WS 71

type strain: yes

Morphology

cell morphology

  • @ref: 24991
  • gram stain: negative
  • cell length: 0.8-1.2 µm
  • cell width: 0.2-0.4 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 24991
  • colony size: 1 mm
  • colony color: pale-pink
  • colony shape: circular
  • incubation period: 3 days
  • medium used: R2A agar (Reasoner's 2A agar)

Culture and growth conditions

culture medium

@refnamegrowth
24991Reasoner's 2A agar (R2A)yes
24991NA (Nutrient Agar)yes
24991PC (plate count) agaryes
24991TSA (Trypticase soy agar)yes

culture temp

@refgrowthtypetemperaturerange
24991positivegrowth20.0-37.0
24991positiveoptimum25mesophilic
67770positivegrowth25mesophilic

culture pH

@refabilitytypepHPH range
24991positivegrowth5.0-9.0alkaliphile
24991positiveoptimum6

Physiology and metabolism

oxygen tolerance

  • @ref: 24991
  • oxygen tolerance: aerobe

spore formation

  • @ref: 24991
  • spore formation: no

halophily

@refsalttested relationconcentrationgrowth
24991NaCloptimum0 %
24991NaClgrowth0.0-1.0 %(w/v)positive

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2499117521(-)-quinic acid-respiration
24991167632-oxobutanoate-respiration
24991370543-hydroxybutyrate-respiration
2499117925alpha-D-glucose-respiration
2499136219alpha-lactose-respiration
2499185146carboxymethylcellulose-hydrolysis
24991casein-hydrolysis
2499117057cellobiose-respiration
2499116024D-mannose-respiration
2499128053melibiose-respiration
2499116634raffinose-respiration
2499116551D-trehalose-respiration
2499116991dna-hydrolysis
249914853esculin-hydrolysis
2499116865gamma-aminobutyric acid-respiration
2499128066gentiobiose-respiration
2499129042glucose 1-phosphate-respiration
2499117368hypoxanthine-hydrolysis
2499116977L-alanine-respiration
2499129985L-glutamate-respiration
2499115603L-leucine-respiration
2499115729L-ornithine-respiration
2499117203L-proline-respiration
2499116857L-threonine-respiration
2499117895L-tyrosine-hydrolysis
249916359lactulose-respiration
2499137657methyl D-glucoside-respiration
2499175146monomethyl succinate-respiration
2499128017starch-hydrolysis
2499130031succinate-respiration
2499117992sucrose-respiration
2499132528turanose-respiration
2499116199urea-hydrolysis
2499116704uridine-respiration
2499115318xanthine-hydrolysis
2499137166xylan-hydrolysis
2499117632nitrate+reduction
2499153424tween 20+hydrolysis
2499153426tween 80+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.
2499128971ampicillinyesyes10 µg
2499117698chloramphenicolyesyes30 µg
2499148923erythromycinyesyes15 µg
2499118208penicillin gyesyes10 Unit
2499117076streptomycinyesyes10 µg
2499128001vancomycinyesyes30 µg

metabolite production

  • @ref: 24991
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

enzymes

@refvalueactivityec
24991acid phosphatase+3.1.3.2
24991alkaline phosphatase+3.1.3.1
24991alpha-chymotrypsin-3.4.21.1
24991alpha-fucosidase-3.2.1.51
24991alpha-galactosidase-3.2.1.22
24991alpha-glucosidase-3.2.1.20
24991alpha-mannosidase-3.2.1.24
24991beta-galactosidase-3.2.1.23
24991beta-glucosidase-3.2.1.21
24991beta-glucuronidase-3.2.1.31
24991catalase+1.11.1.6
24991cystine arylamidase-3.4.11.3
24991cytochrome oxidase-1.9.3.1
24991esterase (C 4)-
24991esterase Lipase (C 8)-
24991leucine arylamidase+3.4.11.1
24991lipase (C 14)-
24991N-acetyl-beta-glucosaminidase-3.2.1.52
24991naphthol-AS-BI-phosphohydrolase-
24991trypsin-3.4.21.4
24991valine arylamidase-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperature
24991freshwaterWoopo wetland in Gyeongnam ProvinceRepublic of KoreaKORAsia35128R2A agar (Reasoner's 2A agar)7 days25
67770Freshwater from the Woopo WetlandRepublic of KoreaKORAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Freshwater

Sequence information

16S sequences

  • @ref: 24991
  • description: Mucilaginibacter sp. WS71 from South Korea 16S ribosomal RNA gene, partial sequence
  • accession: JQ955678
  • length: 1450
  • database: nuccore
  • NCBI tax ID: 1196020

GC content

@refGC-contentmethod
2499139.0-39.8thermal denaturation, midpoint method (Tm)
6777039-39.8thermal denaturation, midpoint method (Tm)

External links

@ref: 24991

culture collection no.: KCTC 32270, JCM 19495

literature

  • topic: Phylogeny
  • Pubmed-ID: 27498538
  • title: Mucilaginibacter puniceus sp. nov., isolated from wetland freshwater.
  • authors: Lee JH, Kim MS, Kang JW, Baik KS, Seong CN
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001389
  • year: 2016
  • mesh: Bacterial Typing Techniques, Bacteroidetes/*classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fresh Water/*microbiology, Phosphatidylethanolamines/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry, *Wetlands
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24991J. H. K. Lee, Mi Sun,Kang, Joo Won,Baik, Keun Sik,Seong, Chi NamMucilaginibacter puniceus sp. nov., isolated from wetland freshwater10.1099/ijsem.0.001389IJSEM 66: 4549-4554 201627498538
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/