Strain identifier

BacDive ID: 13314

Type strain: Yes

Species: Lentzea kentuckyensis

Strain history: CIP <- 2008, DSMZ <- D.P. Labeda, NRRL <- J.M. Donahue, LDDC

NCBI tax ID(s): 360086 (species)

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General

@ref: 12192

BacDive-ID: 13314

DSM-Number: 44909

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming

description: Lentzea kentuckyensis DSM 44909 is an obligate aerobe, spore-forming bacterium that builds an aerial mycelium and was isolated from Equine placenta.

NCBI tax id

  • NCBI tax id: 360086
  • Matching level: species

strain history

@refhistory
12192<- D. P. Labeda, NRRL <- J. M. Donahue, LDDC
67770DSM 44909 <-- D. P. Lebeda LDDC 2876-05.
120401CIP <- 2008, DSMZ <- D.P. Labeda, NRRL <- J.M. Donahue, LDDC

doi: 10.13145/bacdive13314.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Lentzea
  • species: Lentzea kentuckyensis
  • full scientific name: Lentzea kentuckyensis Labeda et al. 2007

@ref: 12192

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Lentzea

species: Lentzea kentuckyensis

full scientific name: Lentzea kentuckyensis Labeda et al. 2007 emend. Nouioui et al. 2018

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
120401positiverod-shaped
69480no90.833

colony morphology

@refcolony colorincubation periodmedium used
20141Golden yellow (1004)10-14 daysISP 2
20141Cream (9001)10-14 daysISP 3
20141Golden yellow (1004)10-14 daysISP 4
20141Lemon yellow (1012)10-14 daysISP 5
20141Lemon yellow (1012)10-14 daysISP 6
20141Lemon yellow (1012)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
20141yesAerial MyceliumCreamISP 2
20141yesAerial MyceliumOchre brownISP 3
20141yesAerial MyceliumCreamISP 4
20141yesAerial MyceliumCreamISP 5
20141yesAerial MyceliumCreamISP 6
20141yesAerial MyceliumCreamISP 7

pigmentation

  • @ref: 31774
  • production: yes

multimedia

  • @ref: 12192
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44909.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12192GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20141ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20141ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20141ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20141ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20141ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20141ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
37841MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
120401CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperature
12192positivegrowth28
31774positivegrowth10-37
31774positiveoptimum23.5
37841positivegrowth30
67770positivegrowth28

Physiology and metabolism

oxygen tolerance

  • @ref: 120401
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 69481
  • spore formation: yes
  • confidence: 97

halophily

  • @ref: 31774
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: <7 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2014117234glucose+
2014122599arabinose+
2014117992sucrose+
2014118222xylose-
2014117268myo-inositol+
2014129864mannitol+
2014128757fructose+
2014126546rhamnose+
2014116634raffinose+
2014162968cellulose+
3177422599arabinose+carbon source
3177417057cellobiose+carbon source
3177416947citrate+carbon source
3177423652dextrin+carbon source
3177428757fructose+carbon source
3177428260galactose+carbon source
3177417234glucose+carbon source
3177417754glycerol+carbon source
3177424996lactate+carbon source
3177417716lactose+carbon source
3177425115malate+carbon source
3177417306maltose+carbon source
3177429864mannitol+carbon source
3177437684mannose+carbon source
3177428053melibiose+carbon source
3177417268myo-inositol+carbon source
3177416634raffinose+carbon source
3177426546rhamnose+carbon source
3177417814salicin+carbon source
3177430911sorbitol+carbon source
3177430031succinate+carbon source
3177417992sucrose+carbon source
3177427082trehalose+carbon source
3177418222xylose+carbon source
317744853esculin+hydrolysis
12040117632nitrate-reduction
12040116301nitrite-reduction

metabolite production

  • @ref: 120401
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
31774gelatinase+
31774urease+3.5.1.5
120401oxidase-
120401catalase+1.11.1.6
120401urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
20141+++-++-++++++++++++
120401+++-+--++++-+++++---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
12192Equine placentaKentucky, LexingtonUSAUSANorth America
67770Equine placentaLexington, KYUSAUSANorth America
120401Animal, Equine placentaLexington, KentuckyUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Equidae (Horse)
#Host Body Product#Urogenital tract

taxonmaps

  • @ref: 69479
  • File name: preview.99_24893.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_840;97_982;98_2059;99_24893&stattab=map
  • Last taxonomy: Lentzea
  • 16S sequence: DQ291145
  • Sequence Identity:
  • Total samples: 116
  • soil counts: 49
  • aquatic counts: 4
  • animal counts: 5
  • plant counts: 58

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
121921Risk group (German classification)
201411German classification
1204011Risk group (French classification)

Sequence information

16S sequences

  • @ref: 12192
  • description: Lentzea kentuckyensis strain NRRL B-24416 16S ribosomal RNA gene, partial sequence
  • accession: DQ291145
  • length: 1419
  • database: nuccore
  • NCBI tax ID: 360086

Genome sequences

  • @ref: 67770
  • description: Lentzea kentuckyensis NRRL B-24416
  • accession: GCA_002150765
  • assembly level: scaffold
  • database: ncbi
  • NCBI tax ID: 360086

GC content

  • @ref: 67770
  • GC-content: 68.8
  • method: genome sequence analysis

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes97no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes87.197no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no97.842no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes86.444no
69480spore-formingspore-formingAbility to form endo- or exosporesyes81.242no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno97yes
69480flagellatedmotile2+Ability to perform flagellated movementno90.833no

External links

@ref: 12192

culture collection no.: DSM 44909, CIP 109797, JCM 14913, LDDC 2876-05, NRRL B-24416

straininfo link

  • @ref: 82515
  • straininfo: 294129

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17684256Lentzea kentuckyensis sp. nov., of equine origin.Labeda DP, Donahue JM, Sells SF, Kroppenstedt RMInt J Syst Evol Microbiol10.1099/ijs.0.64245-02007Actinomycetales/classification/genetics/*isolation & purification, Animals, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Female, Horses/*microbiology, Molecular Sequence Data, Phylogeny, Placenta/microbiology, Pregnancy, RNA, Ribosomal, 16S/geneticsGenetics
Phylogeny22140164Lentzea jiangxiensis sp. nov., isolated from acidic soil.Li X, Zhang L, Ding Y, Gao Y, Ruan J, Huang YInt J Syst Evol Microbiol10.1099/ijs.0.033795-02011Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil MicrobiologyGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
12192Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44909)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44909
20141Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44909.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31774Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2804728776041
37841Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7580
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
82515Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID294129.1StrainInfo: A central database for resolving microbial strain identifiers
120401Curators of the CIPCollection of Institut Pasteur (CIP 109797)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109797