Strain identifier

BacDive ID: 133137

Type strain: Yes

Species: Streptomyces pini

Strain Designation: PL19

NCBI tax ID(s): 1520580 (species)

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General

@ref: 24902

BacDive-ID: 133137

keywords: genome sequence, 16S sequence, Bacteria, aerobe, chemoorganotroph, spore-forming, Gram-positive, filament-shaped

description: Streptomyces pini PL19 is an aerobe, chemoorganotroph, spore-forming bacterium that was isolated from Scot pine needle-like leaves.

NCBI tax id

  • NCBI tax id: 1520580
  • Matching level: species

doi: 10.13145/bacdive133137.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces pini
  • full scientific name: Streptomyces pini Madhaiyan et al. 2016

@ref: 24902

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Streptomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces pini

strain designation: PL19

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
24902positivefilament-shaped
69480no93.771
69480positive100

Culture and growth conditions

culture medium

  • @ref: 24902
  • name: Reasoner's 2A agar (R2A)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
24902positivegrowth25.0-45.0
24902positiveoptimum30mesophilic

culture pH

@refabilitytypepHPH range
24902positivegrowth6.0-11.0alkaliphile
24902positiveoptimum7
24902positivegrowth5
24902positivegrowth11

Physiology and metabolism

oxygen tolerance

  • @ref: 24902
  • oxygen tolerance: aerobe

nutrition type

  • @ref: 24902
  • type: chemoorganotroph

spore formation

@refspore descriptiontype of sporespore formationconfidence
24902spiral,rough to warty surfacesporeyes
69481yes100
69480yes100

halophily

  • @ref: 24902
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0.0-8.0 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2490217057cellobiose-carbon source
2490216988D-ribose-carbon source
2490217268myo-inositol-carbon source
2490262345L-rhamnose-carbon source
2490217309pectin-hydrolysis
2490235020tributyrin-hydrolysis
24902167632-oxobutanoate+carbon source
24902620642,3-butanediol+carbon source
2490222599arabinose+carbon source
2490285146carboxymethylcellulose+hydrolysis
24902casein+hydrolysis
2490215824D-fructose+carbon source
2490212936D-galactose+carbon source
2490217634D-glucose+carbon source
2490216899D-mannitol+carbon source
2490227605D-psicose+carbon source
2490216443D-tagatose+carbon source
2490223652dextrin+carbon source
249025291gelatin+hydrolysis
2490228087glycogen+carbon source
2490218183L-pyroglutamic acid+carbon source
2490217306maltose+carbon source
2490255507methyl alpha-D-galactoside+carbon source
2490237657methyl D-glucoside+carbon source
2490215361pyruvate+carbon source
2490216634raffinose+carbon source
2490228017starch+hydrolysis
2490230031succinate+carbon source
2490253423tween 40+carbon source
2490216704uridine+carbon source
2490218222xylose+carbon source

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
249029215spectinomycinyesyes25 µg/mL
2490217833gentamicinyesyes25 µg/mL
249026104kanamycinyesyes25 µg/mL
2490250845doxycyclineyesyes25 µg/mL
2490248923erythromycinyesyes25 µg/mL
2490228077rifampicinyesyes25 µg/mL
2490228368novobiocinyesyes25 µg/mL
2490228001vancomycinyesyes25 µg/mL
2490227902tetracyclineyesyes50 µg/mL
2490228669bacitracinyesyes50 µg/mL
249028309polymyxin byesyes50 µg/mL
24902100147nalidixic acidyesyes500 µg/mL
2490228971ampicillinyesyes500 µg/mL
24902204928cefotaximeyesyes500 µg/mL
2490217698chloramphenicolyesyes500 µg/mL
249023393carbenicillinyesyes500 µg/mL

Isolation, sampling and environmental information

isolation

  • @ref: 24902
  • sample type: Scot pine needle-like leaves
  • geographic location: TNAU campus, Coimbatore
  • country: India
  • origin.country: IND
  • continent: Asia
  • enrichment culture: ammonium mineral salts medium
  • enrichment culture composition: amended with 0.5 % (v/v) methanol as carbon source and cycloheximide (10 µg ml-1)
  • isolation procedure: leaf imprinting method

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Tree
#Host Body-Site#Plant#Leaf (Phyllosphere)

taxonmaps

  • @ref: 69479
  • File name: preview.99_32948.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_2143;97_2583;98_10732;99_32948&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: EU912466
  • Sequence Identity:
  • Total samples: 9
  • soil counts: 6
  • aquatic counts: 1
  • animal counts: 1
  • plant counts: 1

Sequence information

16S sequences

  • @ref: 24902
  • description: Streptomyces pini strain PL19 16S ribosomal RNA gene, partial sequence
  • accession: EU912466
  • length: 1436
  • database: nuccore
  • NCBI tax ID: 1520580

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces pini PL19GCA_900114215scaffoldncbi1520580
66792Streptomyces pini PL192654587880draftimg1520580

GC content

  • @ref: 24902
  • GC-content: 69.5
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno94.014no
flagellatedno97.886no
gram-positiveyes88.953yes
anaerobicno97.952yes
aerobicyes84.764yes
halophileno80.815no
spore-formingyes94.618yes
glucose-fermentno87.293no
thermophileno95.863yes
glucose-utilyes87.862yes

External links

@ref: 24902

culture collection no.: NRRL B-24728, ICMP 17783

literature

  • topic: Phylogeny
  • Pubmed-ID: 27469580
  • title: Streptomyces pini sp. nov., an actinomycete isolated from phylloplane of pine (Pinus sylvestris L.) needle-like leaves.
  • authors: Madhaiyan M, Poonguzhali S, Saravanan VS, Duraipandiyan V, Al-Dhabi NA, Pragatheswari D, Santhanakrishnan P, Kim SJ, Weon HY, Kwon SW
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001336
  • year: 2016
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, India, Nucleic Acid Hybridization, *Phylogeny, Pinus sylvestris/*microbiology, Plant Leaves/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24902M. P. Madhaiyan, Selvaraj,Saravanan, Venkatakrishnan Sivaraj,Duraipandiyan, Veeramuthu,Al-Dhabi, Naif Abdullah,Pragatheswari, Dhandapani,Santhanakrishnan, Palani,Kim, Soo-Jin,Weon, Hang-Yeon,Kwon, Soon-WoStreptomyces pini sp. nov., an actinomycete isolated from phylloplane of pine (Pinus sylvestris L.) needle-like leaves10.1099/ijsem.0.001336IJSEM 66: 4204-4210 201627469580
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1