General

@ref: 24864

BacDive-ID: 133076

DSM-Number: 29990

keywords: 16S sequence, Bacteria, obligate anaerobe, thermophilic, Gram-positive, motile, rod-shaped

description: Desulfitispora elongata DSM 29990 is an obligate anaerobe, thermophilic, Gram-positive bacterium that was isolated from sediment of a soda lake.

NCBI tax id

• NCBI tax id: 1664035
• Matching level: species

strain history

• @ref: 24864
• history: <- D. Sorokin, Dept. Biotechnol., Delft Univ. Technol., Netherlands; Acr1 <- D. Sorokin {2014}

doi: 10.13145/bacdive133076.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/bacillota
• domain: Bacteria
• phylum: Bacillota
• class: Clostridia
• order: Eubacteriales
• family: Peptococcaceae
• genus: Desulfitispora
• species: Desulfitispora elongata
• full scientific name: Desulfitispora elongata Sorokin and Chernyh 2017

@ref: 24864

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Peptococcaceae

genus: Desulfitispora

species: Desulfitispora elongata

full scientific name: Desulfitispora elongata Sorokin and Chernyh 2017

type strain: yes

Morphology

cell morphology

• @ref: 25180
• gram stain: positive
• cell length: 3.0-25 µm
• cell width: 0.8-1.0 µm
• cell shape: rod-shaped
• motility: yes
• flagellum arrangement: peritrichous

Culture and growth conditions

culture medium

• @ref: 24864
• name: DESULFONATRONUM THIOAUTOTROPHICUM MEDIUM (DSMZ Medium 1166)
• growth: yes
• link: https://mediadive.dsmz.de/medium/1166
• composition: Name: DESULFONATRONUM THIOAUTOTROPHICUM MEDIUM (DSMZ Medium 1166; with strain-specific modifications) Composition: Na2CO3 21.9342 g/l NaHCO3 7.97607 g/l NaCl 5.98205 g/l Na2S2O3 x 5 H2O 4.98504 g/l Na2SO4 2.79163 g/l K2HPO4 0.997009 g/l Na2S x 9 H2O 0.239282 g/l NH4Cl 0.199402 g/l MgCl2 x 6 H2O 0.199402 g/l Yeast extract 0.0498504 g/l EDTA 0.00498504 g/l FeSO4 x 7 H2O 0.00219342 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000199402 g/l ZnSO4 x 7 H2O 9.97009e-05 g/l Pyridoxine hydrochloride 9.97009e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l MnCl2 x 4 H2O 2.99103e-05 g/l Na2MoO4 x 2 H2O 2.99103e-05 g/l NiCl2 x 6 H2O 2.99103e-05 g/l CuCl2 x 2 H2O 2.99103e-05 g/l H3BO3 2.99103e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

culture temp

culture pH

Physiology and metabolism

oxygen tolerance

• @ref: 25180
• oxygen tolerance: obligate anaerobe

spore formation

• @ref: 25180
• spore formation: no

halophily

metabolite utilization

Isolation, sampling and environmental information

isolation

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_140894.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_8583;96_54147;97_69835;98_93637;99_140894&stattab=map
• Last taxonomy: Desulfitispora elongata subclade
• 16S sequence: KP657487
• Sequence Identity:
• Total samples: 625
• soil counts: 86
• aquatic counts: 143
• animal counts: 391
• plant counts: 5

Safety information

risk assessment

• @ref: 24864
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 24864
• description: Desulfitispora elongata strain Acr1 16S ribosomal RNA gene, partial sequence
• accession: KP657487
• length: 1491
• database: ena
• NCBI tax ID: 1664035

GC content

• @ref: 24864
• GC-content: 40.3
• method: thermal denaturation, midpoint method (Tm)

External links

@ref: 24864

culture collection no.: DSM 29990, UNIQEM U994, Acr 1

straininfo link

• @ref: 91341
• straininfo: 400015

literature

• topic: Phylogeny
• Pubmed-ID: 27902279
• title: Desulfonatronospira sulfatiphila sp. nov., and Desulfitispora elongata sp. nov., two novel haloalkaliphilic sulfidogenic bacteria from soda lakes.
• authors: Sorokin DY, Chernyh NA
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijsem.0.001640
• year: 2017
• mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Deltaproteobacteria/*classification/genetics/isolation & purification, Formates/chemistry, Hydrogen-Ion Concentration, Lakes/*microbiology, Peptococcaceae/*classification/genetics/isolation & purification, *Phylogeny, RNA, Ribosomal, 16S/genetics, Russia, Salinity, Sequence Analysis, DNA, Sulfates/chemistry, Sulfites/chemistry
• topic2: Enzymology

Reference