Strain identifier

BacDive ID: 13300

Type strain: No

Species: Lentzea aerocolonigenes

Strain Designation: C-38383-RK2

Strain history: <- ATCC <- J. A. Bush, C-38383-RK2, (Nocardia aerocolonigenes)

NCBI tax ID(s): 68170 (species)

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General

@ref: 11602

BacDive-ID: 13300

DSM-Number: 44217

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, antibiotic compound production, human pathogen

description: Lentzea aerocolonigenes C-38383-RK2 is a mesophilic human pathogen that produces antibiotic compounds and was isolated from soil.

NCBI tax id

  • NCBI tax id: 68170
  • Matching level: species

strain history

  • @ref: 11602
  • history: <- ATCC <- J. A. Bush, C-38383-RK2, (Nocardia aerocolonigenes)

doi: 10.13145/bacdive13300.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Lentzea
  • species: Lentzea aerocolonigenes
  • full scientific name: Lentzea aerocolonigenes (Labeda 1986 ex Shinobu and Kawato 1960) Nouioui et al. 2018

  • synonyms

    @refsynonym
    20215Saccharothrix aerocolonigenes
    20215Lechevalieria aerocolonigenes
    20215Nocardia aerocolonigenes
    20215Streptomyces aerocolonigenes

@ref: 11602

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Lentzea

species: Lentzea aerocolonigenes

full scientific name: Lentzea aerocolonigenes (Labeda 1986) Nouioui et al. 2018

strain designation: C-38383-RK2

type strain: no

Culture and growth conditions

culture medium

  • @ref: 11602
  • name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/65
  • composition: Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

  • @ref: 11602
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Physiology and metabolism

compound production

@refcompound
11602rebeccamycin
11602bromorebeccamycin
116024' deschlororebeccamycin

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836817632nitrate+reduction
6836830849L-arabinose-fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol-fermentation
6836817634D-glucose-fermentation
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836816301nitriteyes
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382trypsin-3.4.21.4
68382acid phosphatase-3.1.3.2
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
11602-+---+-+/--+/--+/-+/-+/--+/-----

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARANO2
11602+--------++---------+

Isolation, sampling and environmental information

isolation

  • @ref: 11602
  • sample type: soil
  • country: Panama
  • origin.country: PAN
  • continent: Middle and South America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_73395.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_840;97_982;98_1160;99_73395&stattab=map
  • Last taxonomy: Lentzea
  • 16S sequence: FJ887954
  • Sequence Identity:
  • Total samples: 152
  • soil counts: 46
  • aquatic counts: 1
  • animal counts: 4
  • plant counts: 101

Safety information

risk assessment

  • @ref: 11602
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Lechevalieria aerocolonigenes strain DSM 44217 16S ribosomal RNA gene, partial sequence
  • accession: FJ887954
  • length: 1360
  • database: ena
  • NCBI tax ID: 68170

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lentzea aerocolonigenes ATCC 39243651716534draftimg68170
66792Lentzea aerocolonigenes ATCC 39243651716540draftimg68170
66792Lentzea aerocolonigenes ATCC 39243651716742draftimg68170

External links

@ref: 11602

culture collection no.: DSM 44217, ATCC 39243

straininfo link

  • @ref: 82501
  • straininfo: 46025

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny1938615Isolation of a bromo analog of rebeccamycin from Saccharothrix aerocolonigenes.Lam KS, Schroeder DR, Veitch JM, Matson JA, Forenza SJ Antibiot (Tokyo)10.7164/antibiotics.44.9341991*Aminoglycosides, Animals, Anti-Bacterial Agents/*isolation & purification/therapeutic use, *Carbazoles, Chemical Phenomena, Chemistry, Chromatography, High Pressure Liquid, *Indoles, Leukemia P388/drug therapy, Mice, Saccharomycetales/*chemistryEnzymology
Metabolism10866221Cloning and expression of a gene encoding N-glycosyltransferase (ngt) from Saccarothrix aerocolonigenes ATCC39243.Ohuchi T, Ikeda-Araki A, Watanabe-Sakamoto A, Kojiri K, Nagashima M, Okanishi M, Suda HJ Antibiot (Tokyo)10.7164/antibiotics.53.3932000Actinomycetales/*genetics, Amino Acid Sequence, Bacterial Proteins, Base Sequence, Carbazoles/*metabolism, Cloning, Molecular, DNA Fragmentation, Fermentation, Glucosides/*metabolism, Glucosyltransferases/*genetics/metabolism, Glycosylation, Glycosyltransferases/*genetics/metabolism, Indoles/*metabolism, Molecular Sequence Data, Molecular Structure, Open Reading Frames, Plasmids, Streptomyces/geneticsEnzymology
Phylogeny11269705Production, isolation and structure determination of novel fluoroindolocarbazoles from Saccharothrix aerocolonigenes ATCC 39243.Lam KS, Schroeder DR, Veitch JM, Colson KL, Matson JA, Rose WC, Doyle TW, Forenza SJ Antibiot (Tokyo)10.7164/antibiotics.54.12001Actinomycetales/chemistry/*metabolism, *Aminoglycosides, Animals, Anti-Bacterial Agents/biosynthesis/isolation & purification/pharmacology, Antibiotics, Antineoplastic/biosynthesis/*chemistry/*isolation & purification/pharmacology, *Carbazoles, Dose-Response Relationship, Drug, Drug Screening Assays, Antitumor, Female, *Indoles, Leukemia, Experimental/drug therapy, Mice, Mice, Inbred BALB C, Mice, Inbred DBA, Molecular Structure, Pilot ProjectsEnzymology
Enzymology11983340The biosynthetic gene cluster for the antitumor rebeccamycin: characterization and generation of indolocarbazole derivatives.Sanchez C, Butovich IA, Brana AF, Rohr J, Mendez C, Salas JAChem Biol10.1016/s1074-5521(02)00126-62002Actinomycetales/enzymology, *Aminoglycosides, Anti-Bacterial Agents/*biosynthesis, Antineoplastic Agents, Carbazoles, Chromosome Mapping, Cloning, Molecular, Glycosyltransferases/genetics/metabolism, Indoles, Magnetic Resonance Spectroscopy, Molecular Sequence Data, *Multigene Family, Sequence Analysis, DNA, Streptomyces/geneticsGenetics
Metabolism12619684Characterization of the biosynthetic gene cluster of rebeccamycin from Lechevalieria aerocolonigenes ATCC 39243.Onaka H, Taniguchi S, Igarashi Y, Furumai TBiosci Biotechnol Biochem10.1271/bbb.67.1272003*Aminoglycosides, Anti-Bacterial Agents/*biosynthesis, Bacteria/genetics/growth & development/metabolism, *Carbazoles, Chromatography, High Pressure Liquid, Cloning, Molecular, DNA, Recombinant/biosynthesis/genetics, Gram-Positive Bacteria/growth & development/*metabolism, *Indoles, Magnetic Resonance Spectroscopy, Multigene Family/*genetics, Plasmids, Spectrophotometry, UltravioletEnzymology
Metabolism15743957Molecular analysis of the rebeccamycin L-amino acid oxidase from Lechevalieria aerocolonigenes ATCC 39243.Nishizawa T, Aldrich CC, Sherman DHJ Bacteriol10.1128/JB.187.6.2084-2092.20052005Actinomycetales/*enzymology/*genetics, Amino Acid Oxidoreductases/*genetics/isolation & purification/*metabolism, Amino Acid Sequence, Carbazoles/chemistry/*metabolism, Gene Expression Regulation, Bacterial, Gene Expression Regulation, Enzymologic, Indoles/chemistry/*metabolism, L-Amino Acid Oxidase, Molecular Biology, Phylogeny, Substrate Specificity, Tryptophan/metabolismEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11602Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44217)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44217
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82501Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46025.1StrainInfo: A central database for resolving microbial strain identifiers