Strain identifier

BacDive ID: 132995

Type strain: Yes

Species: Wenyingzhuangia fucanilytica

Strain Designation: CZ1127

Strain history: <- Yaoguang chang, Ocean Univ. of China

NCBI tax ID(s): 1790137 (species)

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General

@ref: 24783

BacDive-ID: 132995

DSM-Number: 100787

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Wenyingzhuangia fucanilytica CZ1127 is an obligate aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from From shallow coastal seawater of Jiaozhou Bay.

NCBI tax id

  • NCBI tax id: 1790137
  • Matching level: species

strain history

@refhistory
24783<- Y Chang, Ocean Univ. China, Qingdao, P. R. China; CZ1127
67771<- Yaoguang chang, Ocean Univ. of China

doi: 10.13145/bacdive132995.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Wenyingzhuangia
  • species: Wenyingzhuangia fucanilytica
  • full scientific name: Wenyingzhuangia fucanilytica Chen et al. 2016

@ref: 24783

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Wenyingzhuangia

species: Wenyingzhuangia fucanilytica

full scientific name: Wenyingzhuangia fucanilytica Chen et al. 2016

strain designation: CZ1127

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43118negative0.8-2.0 µm0.4-0.5 µmrod-shapedno
67771rod-shapedno
67771negative
69480negative99.991

colony morphology

  • @ref: 43118
  • colony size: 1.0-3.0 mm
  • colony color: yellow
  • colony shape: circular
  • incubation period: 3 days
  • medium used: R2ASA

pigmentation

  • @ref: 43118
  • production: yes
  • name: carotenoids

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
43118R2ASAyesR2A (BD Difco) containing 3 % (w/v) NaCl
43118marine 2216 agar (MA)yes
24783BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yesName: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled waterhttps://mediadive.dsmz.de/medium/514

culture temp

@refgrowthtypetemperaturerange
43118positiveoptimum25mesophilic
43118positivegrowth15-37
24783positivegrowth28mesophilic
67771positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
43118positiveoptimum6.5-7.5
43118positivegrowth5.5-9.0alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43118obligate aerobe
67771aerobe

spore formation

@refspore formationconfidence
69481no99
69480no99.999

halophily

@refsaltgrowthtested relationconcentration
43118NaClpositivegrowth0-4 %(w/v)
43118NaClpositiveoptimum0-3 %(w/v)

observation

  • @ref: 67771
  • observation: quinones: MK-6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
431185181fucoidan+assimilation
4311817895L-tyrosine-hydrolysis
43118casein-hydrolysis
4311853424tween 20-hydrolysis
4311853423tween 40-hydrolysis
4311853425tween 60-hydrolysis
4311853426tween 80-hydrolysis
431184853esculin+hydrolysis
4311817632nitrate-reduction
4311817234glucose-fermentation
431185291gelatin-hydrolysis
4311827689decanoate-assimilation
4311817128adipate-assimilation
4311825115malate-assimilation
4311816947citrate-assimilation
4311818401phenylacetate-assimilation
4311829016arginine-hydrolysis
4311865327D-xylose+carbon source
4311812936D-galactose+carbon source
4311817634D-glucose+carbon source
4311815824D-fructose+carbon source
4311816899D-mannitol+carbon source
4311816024D-mannose+carbon source
4311862345L-rhamnose+carbon source
4311827613amygdalin+carbon source
431184853esculin+carbon source
4311817057cellobiose+carbon source
4311817716lactose+carbon source
4311817992sucrose+carbon source
4311815443inulin+carbon source
4311816634raffinose+carbon source
4311862318D-lyxose+carbon source
4311818287L-fucose+carbon source
4311828017starch+carbon source
4311817754glycerol-carbon source
4311817113erythritol-carbon source
4311817108D-arabinose-carbon source
4311830849L-arabinose-carbon source
4311816988D-ribose-carbon source
4311865328L-xylose-carbon source
4311815963ribitol-carbon source
4311817266L-sorbose-carbon source
4311843943methyl alpha-D-mannoside-carbon source
4311874863methyl beta-D-xylopyranoside-carbon source
43118320061methyl alpha-D-glucopyranoside-carbon source
4311816813galactitol-carbon source
4311817924D-sorbitol-carbon source
43118506227N-acetylglucosamine-carbon source
4311818305arbutin-carbon source
4311817814salicin-carbon source
4311817306maltose-carbon source
4311828053melibiose-carbon source
4311827082trehalose-carbon source
431186731melezitose-carbon source
4311828087glycogen-carbon source
4311817151xylitol-carbon source
4311828066gentiobiose-carbon source
4311832528turanose-carbon source
4311816443D-tagatose-carbon source
4311828847D-fucose-carbon source
4311822605arabinitol-carbon source
4311824265gluconate-carbon source
4311817268myo-inositol-carbon source
43118168082-dehydro-D-gluconate-carbon source
43118581435-dehydro-D-gluconate-carbon source

metabolite production

@refChebi-IDmetaboliteproduction
4311816136hydrogen sulfideno
4311835581indoleno

enzymes

@refvalueactivityec
43118catalase+1.11.1.6
43118cytochrome oxidase+1.9.3.1
43118alkaline phosphatase+3.1.3.1
43118leucine arylamidase+3.4.11.1
43118acid phosphatase+3.1.3.2
43118alpha-glucosidase+3.2.1.20
43118beta-galactosidase+3.2.1.23
43118alpha-fucosidase+3.2.1.51
43118naphthol-AS-BI-phosphohydrolase+
43118esterase (C 4)-
43118esterase Lipase (C 8)-
43118lipase (C 14)-
43118valine arylamidase-
43118cystine arylamidase-3.4.11.3
43118trypsin-3.4.21.4
43118alpha-chymotrypsin-3.4.21.1
43118alpha-galactosidase-3.2.1.22
43118beta-glucuronidase-3.2.1.31
43118beta-glucosidase-3.2.1.21
43118beta-mannosidase-3.2.1.25
43118N-acetyl-beta-glucosaminidase-3.2.1.52
43118urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43118C16:03.5
    43118C13:0 iso3.8
    43118C15:0 iso17.6
    43118C15:1 iso G6.3
    43118C15:1ω6c1.6
    43118C20:4ω6,9,12,15c3
    43118C15:0 3OH1.9
    43118C16:0 3OH3.7
    43118C15:0 iso 3OH12.2
    43118C17:0 iso 3OH17.4
    43118C16:1ω6c / C16:1ω7c13.1
  • type of FA analysis: whole cell analysis
  • incubation medium: marine broth 2216 (MB)
  • agar/liquid: liquid
  • incubation temperature: 25
  • software version: Sherlock 6.0
  • system: MIS MIDI
  • instrument: GC 6890 (Agilent)
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
67771From shallow coastal seawater of Jiaozhou BayQingdaoChinaCHNAsia
24783shallow coastal seawaterQingdao, Jiaozhou BayChinaCHNAsia
43118shallow coastal seawater sampleJiaozhou Bay, QingdaoChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Terrestrial#Coast

Safety information

risk assessment

  • @ref: 24783
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24783
  • description: Wenyingzhuangia fucanilytica strain CZ1127 16S ribosomal RNA gene, partial sequence
  • accession: FJ526380
  • length: 1485
  • database: ena
  • NCBI tax ID: 1790137

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Wenyingzhuangia fucanilytica CZ1127GCA_001697185completencbi1790137
66792Wenyingzhuangia fucanilytica strain CZ11271790137.5completepatric1790137
66792Wenyingzhuangia fucanilytica CZ11272718217755completeimg1790137

GC content

@refGC-contentmethod
2478332.2high performance liquid chromatography (HPLC)
6777132.2

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
flagellatedno95.417no
gram-positiveno97.677yes
anaerobicno99.643yes
halophileno72.055no
spore-formingno97.393no
glucose-utilyes84.805yes
aerobicyes86.912no
thermophileno98.246yes
motileno91.622yes
glucose-fermentno88.412yes

External links

@ref: 24783

culture collection no.: DSM 100787, CCTCC AB 2015089, KCTC 42864

straininfo link

  • @ref: 91271
  • straininfo: 403726

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27220912Wenyingzhuangia fucanilytica sp. nov., a sulfated fucan utilizing bacterium isolated from shallow coastal seawater.Chen F, Chang Y, Dong S, Xue CInt J Syst Evol Microbiol10.1099/ijsem.0.0011842016Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacteriaceae/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, Polysaccharides/*metabolism, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
30248971Fucoidan Sulfatases from Marine Bacterium Wenyingzhuangia fucanilytica CZ1127(T).Silchenko AS, Rasin AB, Zueva AO, Kusaykin MI, Zvyagintseva TN, Kalinovsky AI, Kurilenko VV, Ermakova SPBiomolecules10.3390/biom80400982018Bacteria/chemistry/genetics, Escherichia coli/genetics, Polysaccharides/biosynthesis/chemistry/*genetics, Structure-Activity Relationship, Substrate Specificity, Sulfatases/biosynthesis/chemistry/*genetics
Metabolism32827616Expression and biochemical characterization of two recombinant fucoidanases from the marine bacterium Wenyingzhuangia fucanilytica CZ1127(T).Zueva AO, Silchenko AS, Rasin AB, Kusaykin MI, Usoltseva RV, Kalinovsky AI, Kurilenko VV, Zvyagintseva TN, Thinh PD, Ermakova SPInt J Biol Macromol10.1016/j.ijbiomac.2020.08.1312020Bacterial Proteins/genetics/metabolism, Cloning, Molecular, Escherichia coli/genetics/growth & development, Flavobacteriaceae/*enzymology/genetics, Gene Expression Regulation, Bacterial, Gene Expression Regulation, Enzymologic, Glycoside Hydrolases/*genetics/*metabolism, Hydrolysis, Magnetic Resonance Spectroscopy, Polysaccharides/*chemistry, Recombinant Proteins/metabolism, Substrate SpecificityEnzymology
32849348Discovery and Characterization of an Endo-1,3-Fucanase From Marine Bacterium Wenyingzhuangia fucanilytica: A Novel Glycoside Hydrolase Family.Shen J, Chang Y, Zhang Y, Mei X, Xue CFront Microbiol10.3389/fmicb.2020.016742020
Enzymology34364583Discovery of a fucoidan endo-4O-sulfatase: Regioselective 4O-desulfation of fucoidans and its effect on anticancer activity in vitro.Silchenko AS, Rasin AB, Zueva AO, Kusaykin MI, Zvyagintseva TN, Rubtsov NK, Ermakova SPCarbohydr Polym10.1016/j.carbpol.2021.1184492021Amino Acid Sequence, Antineoplastic Agents/chemical synthesis/*pharmacology, Bacterial Proteins/chemistry/isolation & purification, Cell Line, Tumor, Drug Screening Assays, Antitumor, Flavobacteriaceae/enzymology, Humans, Molecular Structure, Polysaccharides/chemical synthesis/*pharmacology, Substrate Specificity, Sulfatases/*chemistry/isolation & purificationPhylogeny
Enzymology34421852Cloning, Heterologous Expression, and Characterization of a betakappa-Carrageenase From Marine Bacterium Wenyingzhuangia funcanilytica: A Specific Enzyme for the Hybrid Carrageenan-Furcellaran.Cao S, Zhang Y, Chen G, Shen J, Han J, Chang Y, Xiao H, Xue CFront Microbiol10.3389/fmicb.2021.6972182021
35940339Fucoidan-active alpha-L-fucosidases of the GH29 and GH95 families from a fucoidan degrading cluster of the marine bacterium Wenyingzhuangia fucanilytica.Silchenko AS, Rubtsov NK, Zueva AO, Kusaykin MI, Rasin AB, Ermakova SPArch Biochem Biophys10.1016/j.abb.2022.1093732022*Flavobacteriaceae, Fucose, Polysaccharides, Substrate Specificity, *alpha-L-Fucosidase

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24783Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100787Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100787)
43118Feng Chen, Yaoguang Chang, Shujun Dong, Changhu XueWenyingzhuangia fucanilytica sp. nov., a sulfated fucan utilizing bacterium isolated from shallow coastal seawater10.1099/ijsem.0.001184IJSEM 66: 3270-3275 201627220912
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
91271Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID403726.1