Strain identifier

BacDive ID: 132993

Type strain: Yes

Species: Allohahella marinimesophila

Strain Designation: H94

Strain history: X.-H. Zhang H94 <-- R. Zhao H94.

NCBI tax ID(s): 1054972 (species)

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General

@ref: 24781

BacDive-ID: 132993

DSM-Number: 24882

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped, colony-forming

description: Allohahella marinimesophila H94 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from seawater from an amphioxus breeding zone.

NCBI tax id

  • NCBI tax id: 1054972
  • Matching level: species

strain history

@refhistory
24781<- X.-H. Zhang, Ocean Univ., Dept. of Marine Biology, China, Qingdao, P. R. China; H94 <- R. Zhao
67770X.-H. Zhang H94 <-- R. Zhao H94.

doi: 10.13145/bacdive132993.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Hahellaceae
  • genus: Allohahella
  • species: Allohahella marinimesophila
  • full scientific name: Allohahella marinimesophila Han et al. 2016

@ref: 24781

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Hahellaceae

genus: Allohahella

species: Allohahella marinimesophila

full scientific name: Allohahella marinimesophila Han et al. 2016

strain designation: H94

type strain: yes

Morphology

cell morphology

  • @ref: 43942
  • gram stain: negative
  • cell length: 1.6-1.8 µm
  • cell width: 0.55-0.65 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: gliding

colony morphology

  • @ref: 43942
  • colony size: 1-2 mm
  • colony color: cream
  • colony shape: circular
  • incubation period: 2 days
  • medium used: Marine agar 2216

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43942Marine agar (MA)yes
24781R2A MEDIUM WITH SEAWATER 75% (DSMZ Medium 1351)yeshttps://mediadive.dsmz.de/medium/1351Name: R2A MEDIUM WITH SEAWATER (DSMZ Medium 1351) Composition: Agar 20.0 g/l Yeast extract 0.5 g/l Proteose peptone 0.5 g/l Casamino acids 0.5 g/l Glucose 0.5 g/l Starch 0.5 g/l Na-pyruvate 0.3 g/l K2HPO4 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Sea water Distilled water

culture temp

@refgrowthtypetemperaturerange
43942positivegrowth4-37
43942positiveoptimum28mesophilic
24781positivegrowth15psychrophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
43942positivegrowth6.0-10.0alkaliphile
43942positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 43942
  • oxygen tolerance: aerobe

spore formation

  • @ref: 43942
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
43942NaClpositivegrowth1-12 %(w/v)
43942NaClpositiveoptimum1-2 %(w/v)

observation

  • @ref: 43942
  • observation: quinone Q-9

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4394228053melibiose-carbon source
4394217029chitin-degradation
439425291gelatin-degradation
4394228017starch-degradation
4394253424tween 20-degradation
43942casein-hydrolysis
439425291gelatin-hydrolysis
4394216199urea-hydrolysis
4394217632nitrate-reduction
4394273706bromosuccinate+carbon source
4394215570D-alanine+carbon source
4394221217L-alaninamide+carbon source
4394215971L-histidine+carbon source
4394253426tween 80+degradation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
4394217833gentamicinyesyes10 µg (disc)
439423508ceftazidimeyesyes30 µg (disc)
439428309polymyxin byesyes300 Unit (disc)
43942100241ciprofloxacinyesyes5 µg (disc)
43942100246norfloxacinyesyes10 µg (disc)
4394229007ceftriaxoneyesyes30 µg (disc)
439423770co-trimoxazoleyesyes
439425195furazolidoneyesyes300 µg (disc)
4394217698chloramphenicolyesyes30 µg (disc)
4394228971ampicillinyesyes10 µg (disc)
4394223066cephalosporinyesyes30 µg (disc)
439428232piperacillinyesyes100 µg (disc)
4394248923erythromycinyesyes15 µg (disc)
439423493cefoperazoneyesyes75 µg (disc)
4394228077rifampicinyesyes5 µg (disc)
439427528netilmycinyesyes30 µg (disc)
4394245924trimethoprimyesyes5 µg (disc)
439426104kanamycinyesyes30 µg (disc)
439427731ofloxacinyesyes5 µg (disc)
439427507neomycinyesyes30 µg (disc)
4394228864tobramycinyesyes10 µg (disc)
439422637amikacinyesyes30 µg (disc)
439423515cefuroximeyesyes30 µg (disc)
4394228368novobiocinyesyes30 µg (disc)
4394217076streptomycinyesyes10 µg (disc)

enzymes

@refvalueactivityec
43942catalase+1.11.1.6
43942cytochrome oxidase-1.9.3.1
43942DNase-
43942alginase-3.2.1.16
43942beta-galactosidase+3.2.1.23
43942tryptophan deaminase+4.1.99.1
43942alpha-glucosidase+3.2.1.20
43942leucine arylamidase-3.4.11.1
43942acid phosphatase-3.1.3.2

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43942C10:02.8
    43942C12:02
    43942C12:0 3OH7.3
    43942C14:01
    43942C16:025.2
    43942C16:0 N alcohol2.1
    43942C16:1ω7c / C16:1ω6c6.2
    43942C16:1ω9c10.3
    43942C18:03
    43942C18:1ω9c34.3
    43942C18:3ω6c (6,9,12)4.2
  • type of FA analysis: whole cell analysis
  • incubation medium: Marine agar 2216
  • agar/liquid: agar
  • incubation temperature: 28
  • software version: Sherlock 6.0
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling dateenrichment cultureenrichment culture composition
24781seawater from an amphioxus breeding zonecoastal region of the Yellow Sea (120° 32' E, 36° 5' N; about 10 m deep)ChinaCHNAsia36.0931120.537
43942seawater of an amphioxus breeding zone in the coastal region of Qingdao, Yellow Sea, ChinaQingdao, Yellow SeaChinaCHNAsia36.0833120.5332009-11marine R2A agar0.5 g yeast extract, 0.5 g proteose peptone, 0.5 g Casamino acids, 0.5 g dextrose, 0.5 g soluble starch, 0.3 g sodium pyruvate, 75 % seawater
67770Seawater of an amphioxus breeding zone in the coastal region of QingdaoChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Host#Fishes
#Environmental#Terrestrial#Coast
#Environmental#Aquatic#Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_144092.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_643;96_34182;97_71225;98_95618;99_144092&stattab=map
  • Last taxonomy: Allohahella
  • 16S sequence: HQ834530
  • Sequence Identity:
  • Total samples: 4575
  • soil counts: 1107
  • aquatic counts: 2331
  • animal counts: 983
  • plant counts: 154

Safety information

risk assessment

  • @ref: 24781
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24781
  • description: Hahella sp. H94 16S ribosomal RNA gene, partial sequence
  • accession: HQ834530
  • length: 1437
  • database: ena
  • NCBI tax ID: 1054972

GC content

  • @ref: 24781
  • GC-content: 56.2
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 24781

culture collection no.: DSM 24882, CGMCC 1.10800, JCM 17555

straininfo link

  • @ref: 91269
  • straininfo: 400196

literature

  • topic: Phylogeny
  • Pubmed-ID: 27216767
  • title: Allohahella marinimesophila gen. nov., sp. nov., isolated from seawater and reclassification of Hahella antarctica as Allohahella antarctica comb. nov.
  • authors: Han Y, Zhao R, Yu T, Li Z, Zhang XH
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001174
  • year: 2016
  • mesh: Antarctic Regions, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gammaproteobacteria/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24781Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-24882Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24882)
43942Yanqiong Han, Rui Zhao, Tong Yu, Zhao Li, Xiao-Hua ZhangAllohahella marinimesophila gen. nov., sp. nov., isolated from seawater and reclassification of Hahella antarctica as Allohahella antarctica comb. nov.10.1099/ijsem.0.001174IJSEM 66: 3207-3213 201627216767
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
91269Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID400196.1