Strain identifier

BacDive ID: 132920

Type strain: Yes

Species: Burkholderia pseudomultivorans

Strain Designation: LiPuma AU3207, Vandamme R-48990

Strain history: <- CCUG; CCUG 62895 <- P. A. R. Vandamme, LMG; Vandamme R-48990 <- J. J. LiPuma, Univ. Michigan, USA; LiPuma AU3207

NCBI tax ID(s): 1207504 (species)

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General

@ref: 24708

BacDive-ID: 132920

DSM-Number: 105103

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic

description: Burkholderia pseudomultivorans LiPuma AU3207 is an aerobe, mesophilic bacterium that was isolated from human sputum, patient with cystic fibrosis.

NCBI tax id

  • NCBI tax id: 1207504
  • Matching level: species

strain history

  • @ref: 24708
  • history: <- CCUG; CCUG 62895 <- P. A. R. Vandamme, LMG; Vandamme R-48990 <- J. J. LiPuma, Univ. Michigan, USA; LiPuma AU3207

doi: 10.13145/bacdive132920.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Burkholderia
  • species: Burkholderia pseudomultivorans
  • full scientific name: Burkholderia pseudomultivorans Peeters et al. 2014

@ref: 24708

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Burkholderia

species: Burkholderia pseudomultivorans

full scientific name: Burkholderia pseudomultivorans Peeters et al. 2014

strain designation: LiPuma AU3207, Vandamme R-48990

type strain: yes

Morphology

colony morphology

  • @ref: 62907
  • incubation period: 1 day

Culture and growth conditions

culture medium

  • @ref: 24708
  • name: R2A MEDIUM (DSMZ Medium 830)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/830
  • composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
24708positivegrowth30mesophilic
62907positivegrowth30-37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 62907
  • oxygen tolerance: aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
24708+---+/-+--+++++-+++++++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
24708human sputum, patient with cystic fibrosisPortland, OregonUSAUSANorth America
62907Human sputum,cystic fibrosisOregon,PortlandUSAUSANorth America2001-06-22

isolation source categories

Cat1Cat2Cat3
#Infection#Disease#Cystic fibrosis
#Infection#Patient
#Host#Human
#Host Body Product#Fluids#Sputum

Safety information

risk assessment

  • @ref: 24708
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24708
  • description: Burkholderia pseudomultivorans partial 16S rRNA gene, type strain LMG 26883T
  • accession: HE962386
  • length: 1485
  • database: ena
  • NCBI tax ID: 1207504

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Burkholderia pseudomultivorans LMG 26883GCA_902499075contigncbi1207504
66792Burkholderia pseudomultivorans strain LMG 268831207504.16wgspatric1207504

GC content

  • @ref: 24708
  • GC-content: 67
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes87.243no
gram-positiveno98.52no
anaerobicno98.226no
aerobicyes87.174yes
halophileno94.496no
spore-formingno93.586no
thermophileno99.334yes
glucose-utilyes90.023yes
flagellatedno80.758no
glucose-fermentno88.44no

External links

@ref: 24708

culture collection no.: DSM 105103, CCUG 62895, LMG 26883

straininfo link

  • @ref: 91198
  • straininfo: 378933

literature

  • topic: Phylogeny
  • Pubmed-ID: 23867250
  • title: Burkholderia pseudomultivorans sp. nov., a novel Burkholderia cepacia complex species from human respiratory samples and the rhizosphere.
  • authors: Peeters C, Zlosnik JE, Spilker T, Hird TJ, LiPuma JJ, Vandamme P
  • journal: Syst Appl Microbiol
  • DOI: 10.1016/j.syapm.2013.06.003
  • year: 2013
  • mesh: Bacterial Typing Techniques, Base Composition, Burkholderia cepacia complex/*classification/genetics/*isolation & purification/physiology, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Environmental Microbiology, Fatty Acids/analysis, Humans, Molecular Sequence Data, Multilocus Sequence Typing, Phylogeny, RNA, Ribosomal, 16S/genetics, Rec A Recombinases/genetics, Respiratory System/*microbiology, Rhizosphere
  • topic2: Pathogenicity

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24708Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-105103Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105103)
62907Curators of the CCUGhttps://www.ccug.se/strain?id=62895Culture Collection University of Gothenburg (CCUG) (CCUG 62895)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68369Automatically annotated from API 20NE
91198Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID378933.1