Strain identifier

BacDive ID: 132852

Type strain: Yes

Species: Psychromonas marina

Strain Designation: 4-22

Strain history: CIP <- 2002, NCIMB

NCBI tax ID(s): 88364 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 8.1 (current version)

General

@ref: 24640

BacDive-ID: 132852

DSM-Number: 15302

keywords: 16S sequence, Bacteria, psychrophilic, Gram-negative, oval-shaped

description: Psychromonas marina 4-22 is a psychrophilic, Gram-negative, oval-shaped bacterium that was isolated from Cold current off the Monbetsu coast of the Okhotsk Sea.

NCBI tax id

  • NCBI tax id: 88364
  • Matching level: species

strain history

@refhistory
24640<- JCM; JCM 10501 <- I. Yumoto; 4-22
361742002, NCIMB
67770I. Yumoto 4-22.
120801CIP <- 2002, NCIMB

doi: 10.13145/bacdive132852.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Psychromonadaceae
  • genus: Psychromonas
  • species: Psychromonas marina
  • full scientific name: Psychromonas marina Kawasaki et al. 2002

@ref: 24640

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Psychromonadaceae

genus: Psychromonas

species: Psychromonas marina

full scientific name: Psychromonas marina Kawasaki et al. 2002

strain designation: 4-22

type strain: yes

Morphology

cell morphology

  • @ref: 120801
  • gram stain: negative
  • cell shape: oval-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
24640BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
36174Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
120801CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
24640positivegrowth15psychrophilic
36174positivegrowth18psychrophilic
67770positivegrowth15psychrophilic
120801positivegrowth5-22psychrophilic
120801nogrowth25mesophilic
120801nogrowth30mesophilic
120801nogrowth37mesophilic
120801nogrowth41thermophilic

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
120801NaClpositivegrowth2-4 %
120801NaClnogrowth0 %
120801NaClnogrowth6 %
120801NaClnogrowth8 %
120801NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12080116947citrate-carbon source
1208014853esculin-hydrolysis
12080117632nitrate+reduction
12080116301nitrite-reduction

metabolite production

  • @ref: 120801
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
120801oxidase+
120801beta-galactosidase+3.2.1.23
120801alcohol dehydrogenase-1.1.1.1
120801gelatinase-
120801amylase+
120801caseinase-3.4.21.50
120801catalase+1.11.1.6
120801tween esterase-
120801lecithinase-
120801lipase-
120801lysine decarboxylase-4.1.1.18
120801ornithine decarboxylase-4.1.1.17
120801protease-
120801tryptophan deaminase-
120801urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120801-+---+----++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
24640Cold current off the Monbetsu coast of the Okhotsk SeaHokkaidoJapanJPNAsia
67770Cold current off the Monbetsu coast of the Okhotsk SeaHokkaidoJapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Condition#Psychrophilic (<10°C)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
246401Risk group (German classification)
1208011Risk group (French classification)

Sequence information

16S sequences

  • @ref: 24640
  • description: Psychromonas sp. 4-22 gene for 16S rRNA
  • accession: AB023378
  • length: 1501
  • database: ena
  • NCBI tax ID: 88364

GC content

  • @ref: 67770
  • GC-content: 43.5
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 24640

culture collection no.: DSM 15302, JCM 10501, NCIMB 13792, ATCC BAA 724, IAM 14899, KCTC 12105, NBRC 103166, CIP 107721

straininfo link

  • @ref: 91140
  • straininfo: 60981

literature

  • topic: Phylogeny
  • Pubmed-ID: 12361246
  • title: Psychromonas marina sp. nov., a novel halophilic, facultatively psychrophilic bacterium isolated from the coast of the Okhotsk Sea.
  • authors: Kawasaki K, Nogi Y, Hishinuma M, Nodasaka Y, Matsuyama H, Yumoto I
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-5-1455
  • year: 2002
  • mesh: Alteromonadaceae/*classification/genetics/isolation & purification/metabolism, DNA, Bacterial/chemistry/genetics, Fatty Acids/analysis/chemistry, Genes, Bacterial, Microscopy, Electron, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Species Specificity, Terminology as Topic
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24640Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-15302Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15302)
36174Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5188
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
91140Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID60981.1
120801Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107721Collection of Institut Pasteur (CIP 107721)