Strain identifier

BacDive ID: 132612

Type strain: Yes

Species: Microlunatus nigridraconis

Strain history: <- Yu-Qin Zhang, Institute of Mediicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College

NCBI tax ID(s): 1710544 (species)

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General

@ref: 24401

BacDive-ID: 132612

DSM-Number: 29529

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, coccus-shaped, colony-forming

description: Microlunatus nigridraconis DSM 29529 is an aerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from rhizosphere soil.

NCBI tax id

  • NCBI tax id: 1710544
  • Matching level: species

strain history

@refhistory
24401<- Y.-Q. Zhang, Chinese Acad. Med. Sciences & Peking Union Medical College; CPCC 203993 <- C.-F. Zhang
67771<- Yu-Qin Zhang, Institute of Mediicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College

doi: 10.13145/bacdive132612.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Propionibacteriaceae
  • genus: Microlunatus
  • species: Microlunatus nigridraconis
  • full scientific name: Microlunatus nigridraconis Zhang et al. 2016

@ref: 24401

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Propionibacteriaceae

genus: Microlunatus

species: Microlunatus nigridraconis

full scientific name: Microlunatus nigridraconis Zhang et al. 2016

type strain: yes

Morphology

cell morphology

  • @ref: 43111
  • gram stain: positive
  • cell length: 0.5-0.7 µm
  • cell width: 0.5-0.7 µm
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 43111
  • colony size: 0.5-1.0 mm
  • colony color: ivory-white
  • colony shape: circular
  • incubation period: 5 days
  • medium used: R2A

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43111Reasoner's 2A agar (R2A)yes
43111Trypticase Soy Agar (TSA)yes
43111ISP 2yes
43111ISP 4yes
24401TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43111positiveoptimum28mesophilic
43111positivegrowth15-30
24401positivegrowth28mesophilic
67771positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
43111positivegrowth6.0-10.0alkaliphile
43111positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43111aerobe
67771aerobe

spore formation

  • @ref: 43111
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
43111NaClpositivegrowth0-5 %(w/v)
43111NaClpositiveoptimum0 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4311117632nitrate+reduction
4311128017starch-hydrolysis
4311113705acetoacetate+carbon source
4311117057cellobiose+carbon source
4311123652dextrin+carbon source
4311115824D-fructose+carbon source
4311112936D-galactose+carbon source
4311117306maltose+carbon source
4311116024D-mannose+carbon source
4311128053melibiose+carbon source
4311116634raffinose+carbon source
4311117814salicin+carbon source
4311117924D-sorbitol+carbon source
4311127082trehalose+carbon source
4311132528turanose+carbon source
4311128066gentiobiose+carbon source
4311117754glycerol+carbon source
4311118287L-fucose+carbon source
4311129985L-glutamate+carbon source
4311116651(S)-lactate+carbon source
4311127613amygdalin+builds acid from
4311117108D-arabinose+builds acid from
4311117057cellobiose+builds acid from
4311115824D-fructose+builds acid from
4311112936D-galactose+builds acid from
4311117634D-glucose+builds acid from
4311117716lactose+builds acid from
4311162318D-lyxose+builds acid from
4311117306maltose+builds acid from
4311116024D-mannose+builds acid from
431116731melezitose+builds acid from
4311128053melibiose+builds acid from
4311116634raffinose+builds acid from
4311116988D-ribose+builds acid from
4311117992sucrose+builds acid from
4311116443D-tagatose+builds acid from
4311127082trehalose+builds acid from
4311132528turanose+builds acid from
4311165327D-xylose+builds acid from
4311117113erythritol+builds acid from
43111esculin ferric citrate+builds acid from
4311117754glycerol+builds acid from
4311130849L-arabinose+builds acid from
4311118403L-arabitol+builds acid from
4311165328L-xylose+builds acid from
43111320061methyl alpha-D-glucopyranoside+builds acid from
4311143943methyl alpha-D-mannoside+builds acid from
4311117814salicin+builds acid from
4311117151xylitol+builds acid from
4311128017starch-builds acid from
4311118305arbutin-builds acid from
4311117924D-sorbitol-builds acid from
4311116813galactitol-builds acid from
4311128066gentiobiose-builds acid from
4311128087glycogen-builds acid from
4311117268myo-inositol-builds acid from
4311115443inulin-builds acid from
4311117266L-sorbose-builds acid from
43111506227N-acetylglucosamine-builds acid from
43111potassium 2-dehydro-D-gluconate-builds acid from
43111potassium 5-dehydro-D-gluconate-builds acid from

metabolite production

  • @ref: 43111
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

enzymes

@refvalueactivityec
43111catalase+1.11.1.6
43111cytochrome oxidase+1.9.3.1
43111acid phosphatase+3.1.3.2
43111alkaline phosphatase+3.1.3.1
43111cystine arylamidase+3.4.11.3
43111esterase (C 4)+
43111esterase Lipase (C 8)+
43111leucine arylamidase+3.4.11.1
43111N-acetyl-beta-glucosaminidase+3.2.1.52
43111naphthol-AS-BI-phosphohydrolase+
43111trypsin+3.4.21.4
43111valine arylamidase+
43111alpha-glucosidase+3.2.1.20
43111alpha-mannosidase+3.2.1.24
43111beta-galactosidase+3.2.1.23
43111beta-glucosidase+3.2.1.21
43111beta-glucuronidase+3.2.1.31

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43111C15:0 anteiso40.1
    43111C16:0 iso34.5
    43111C15:0 iso10.1
  • type of FA analysis: whole cell analysis
  • incubation medium: trypticase soy broth (TSB; Difco)
  • agar/liquid: liquid
  • incubation temperature: 28
  • incubation time: 5
  • software version: Sherlock 6.0
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
24401rhizosphere soilHeilongjiang Province, Heilong mountainChinaCHNAsia
43111rhizosphere soilHeilong mountain, Heilongjiang provinceChinaCHNAsiaisolation mediumcellobiose 2 g/l, yeast extract 5 g/l, CaCO3 2 g/l, K2HPO4 1 g/l, MgSO4x7H2O 0.5 g/l, agar 15 g/l, pH 7.2-7.514 days28
67771From SoilChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host Body-Site#Plant#Rhizosphere
#Host#Plants

taxonmaps

  • @ref: 69479
  • File name: preview.99_62033.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_72;96_23153;97_28960;98_36940;99_62033&stattab=map
  • Last taxonomy: Microlunatus
  • 16S sequence: KT694343
  • Sequence Identity:
  • Total samples: 536
  • soil counts: 388
  • aquatic counts: 23
  • animal counts: 62
  • plant counts: 63

Safety information

risk assessment

  • @ref: 24401
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24401
  • description: Microlunatus nigridraconis strain CPCC 203993 16S ribosomal RNA gene, partial sequence
  • accession: KT694343
  • length: 1452
  • database: ena
  • NCBI tax ID: 1710544

GC content

@refGC-contentmethod
4311164.04
2440164.04thermal denaturation, midpoint method (Tm)

External links

@ref: 24401

culture collection no.: DSM 29529, KCTC 29689, NBRC 110715, CPCC 203993

straininfo link

  • @ref: 90926
  • straininfo: 402820

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27306875Microlunatus nigridraconis sp. nov., an actinobacterium from rhizosphere soil.Zhang CF, Ai MJ, Zhao LL, Liu HY, Yu LY, Zhang YQInt J Syst Evol Microbiol10.1099/ijsem.0.0012412016Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Propionibacteriaceae/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny31485839Microlunatus speluncae sp. nov., a novel actinobacterium isolated from a Karstic subterranean environment sample.Xie YG, Fang BZ, Han MX, Liu L, Jiao JY, Zhang XT, Xiao M, Li WJAntonie Van Leeuwenhoek10.1007/s10482-019-01321-42019Actinomycetales/classification/genetics, Base Composition/genetics, Glycolipids/metabolism, Phosphatidylglycerols/metabolism, Phosphatidylinositols/metabolism, Phylogeny, Propionibacteriaceae/classification/*genetics, RNA, Ribosomal, 16S/geneticsMetabolism

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24401Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29529Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29529)
43111Chang-Feng Zhang, Meng-Jie Ai, Li-Li Zhao, Hong-Yu Liu, Li-Yan Yu, Yu-Qin ZhangMicrolunatus nigridraconis sp. nov., an actinobacterium from rhizosphere soil10.1099/ijsem.0.001241IJSEM 66: 3614-3618 201627306875
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
90926Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID402820.1