Strain identifier

BacDive ID: 132551

Type strain: Yes

Species: Racemicystis crocea

Strain history: <- R.P. Awal, Helmholtz Institute for Pharmaceutical Research (HIPS), Saarbrücken, Germany; <- R.P. Awal;

NCBI tax ID(s): 1707966 (species)

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General

@ref: 24340

BacDive-ID: 132551

DSM-Number: 100773

keywords: 16S sequence, Bacteria, aerobe, motile

description: Racemicystis crocea DSM 100773 is an aerobe, motile bacterium that was isolated from dried soil sample of the soil library of the Gesellschaft für Biotechnologische Forschung .

NCBI tax id

  • NCBI tax id: 1707966
  • Matching level: species

strain history

  • @ref: 24340
  • history: <- R.P. Awal, Helmholtz Institute for Pharmaceutical Research (HIPS), Saarbrücken, Germany; <- R.P. Awal;

doi: 10.13145/bacdive132551.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Deltaproteobacteria
  • order: Myxococcales
  • family: Polyangiaceae
  • genus: Racemicystis
  • species: Racemicystis crocea
  • full scientific name: Racemicystis crocea Awal et al. 2016

@ref: 24340

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Myxococcales

family: Polyangiaceae

genus: Racemicystis

species: Racemicystis crocea

full scientific name: Racemicystis crocea Awal et al. 2016

type strain: yes

Morphology

cell morphology

  • @ref: 43979
  • cell length: 3.0-12.0 µm
  • cell width: 1.2-1.5 µm
  • motility: yes
  • flagellum arrangement: gliding

Culture and growth conditions

culture medium

  • @ref: 24340
  • name: VY/2 AGAR (DSMZ Medium 9)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/9
  • composition: Name: VY/2 AGAR (DSMZ Medium 9) Composition: Agar 15.0 g/l Baker's yeast 5.0 g/l CaCl2 x 2 H2O 1.36 g/l Vitamin B12 0.0005 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43979positivegrowth18-37
43979positiveoptimum30mesophilic
24340positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
43979positivegrowth5.5-8.5alkaliphile
43979positiveoptimum7.0-7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 43979
  • oxygen tolerance: aerobe

halophily

  • @ref: 43979
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 1 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
439792509agar+degradation
4397922599arabinose+growth
43979casitone+growth
4397917057cellobiose+growth
4397928757fructose+growth
4397928260galactose+growth
4397917234glucose+growth
4397917306maltose+growth
4397929864mannitol+growth
4397930911sorbitol+growth
43979soyton+growth
4397928017starch+growth
4397918222xylose+growth
43979casein+hydrolysis
439794853esculin+hydrolysis
439795291gelatin+hydrolysis
4397928017starch+hydrolysis
4397953426tween 80+hydrolysis
4397937166xylan+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistant
4397928971ampicillinyesyes
439792790apramycinyesyes
439793393carbenicillinyesyes
4397923066cephalosporinyesyes
4397917833gentamicinyesyes
439796104kanamycinyesyes
439799215spectinomycinyesyes
4397971321fusidateyesyes
4397981419kasugamycinyesyes
4397927902tetracyclineyesyes
439797507neomycinyesyes
4397917076streptomycinyesyes
4397927701oxytetracyclineyesyes
439798309polymyxin byesyes
4397928669bacitracinyesyes
4397924753hygromycinyesyes
4397928864tobramycinyesyes

enzymes

@refvalueactivityec
43979catalase+1.11.1.6
43979cytochrome oxidase+1.9.3.1
43979alkaline phosphatase+3.1.3.1
43979esterase (C 4)+
43979esterase Lipase (C 8)+
43979lipase (C 14)+
43979leucine arylamidase+3.4.11.1
43979valine arylamidase+
43979alpha-chymotrypsin+3.4.21.1
43979acid phosphatase+3.1.3.2
43979naphthol-AS-BI-phosphohydrolase+
43979beta-galactosidase+3.2.1.23
43979beta-glucosidase+3.2.1.21
43979cystine arylamidase-3.4.11.3
43979trypsin-3.4.21.4
43979alpha-galactosidase-3.2.1.22
43979beta-glucuronidase-3.2.1.31
43979alpha-glucosidase-3.2.1.20
43979N-acetyl-beta-glucosaminidase-3.2.1.52
43979alpha-mannosidase-3.2.1.24
43979alpha-fucosidase-3.2.1.51

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentenrichment cultureenrichment culture composition
24340dried soil sample of the soil library of the Gesellschaft für Biotechnologische Forschung (GBF)PhilippinesPHLAsia
43979soil sample collected in 1986 from the PhilippinesPhilippinesPHLAsiamineral salt ST21 agarsupplemented with 25 µg/ml levamisole, 25 µg/ml cycloheximide

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_84771.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_400;96_13255;97_42056;98_54893;99_84771&stattab=map
  • Last taxonomy: Racemicystis crocea
  • 16S sequence: KT591707
  • Sequence Identity:
  • Total samples: 254
  • soil counts: 99
  • aquatic counts: 128
  • animal counts: 22
  • plant counts: 5

Safety information

risk assessment

  • @ref: 24340
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24340
  • description: Racemicystis crocea strain MSr9521 16S ribosomal RNA gene, partial sequence
  • accession: KT591707
  • length: 1549
  • database: ena
  • NCBI tax ID: 1707966

GC content

  • @ref: 24340
  • GC-content: 70.4
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 24340

culture collection no.: DSM 100773, NCCB 100574, MSR MSr9521, MSR 9521

straininfo link

  • @ref: 90874
  • straininfo: 400946

literature

  • topic: Phylogeny
  • Pubmed-ID: 27046779
  • title: Racemicystis crocea gen. nov., sp. nov., a soil myxobacterium in the family Polyangiaceae.
  • authors: Awal RP, Garcia R, Muller R
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001045
  • year: 2016
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Myxococcales/*classification/genetics/isolation & purification, Philippines, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24340Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100773Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100773)
43979Ram Prasad Awal, Ronald Garcia, Rolf MüllerRacemicystis crocea gen. nov., sp. nov., a soil myxobacterium in the family Polyangiaceae10.1099/ijsem.0.001045IJSEM 66: 2389-2395 201627046779
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
90874Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID400946.1