General

@ref: 24291

BacDive-ID: 132502

DSM-Number: 29988

keywords: genome sequence, 16S sequence, Archaea, mesophilic

description: Haloferax namakaokahaiae Mke2.3 is a mesophilic archaeon that was isolated from saline cinders from a seawater splash zone at the top of a lava escarpment.

NCBI tax id

• NCBI tax id: 1748331
• Matching level: species

strain history

• @ref: 24291
• history: <- G. M. King, Louisiana State Univ., Baton Rouge, USA <- S. McDuff

doi: 10.13145/bacdive132502.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Halobacteria
• order: Haloferacales
• family: Haloferacaceae
• genus: Haloferax
• species: Haloferax namakaokahaiae
• full scientific name: Haloferax namakaokahaiae McDuff et al. 2017

@ref: 24291

domain: Archaea

phylum: Euryarchaeota

class: Halobacteria

order: Halobacteriales

family: Haloferacaceae

genus: Haloferax

species: Haloferax namakaokahaiae

full scientific name: Haloferax namakaokahaiae McDuff et al. 2017

strain designation: Mke2.3

type strain: yes

Culture and growth conditions

culture medium

• @ref: 24291
• name: HALOBACTERIA MEDIUM (DSMZ Medium 372)
• growth: yes
• link: https://mediadive.dsmz.de/medium/372
• composition: Name: HALOBACTERIA MEDIUM (DSMZ Medium 372) Composition: NaCl 200.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 20.0 g/l Yeast extract 5.0 g/l Casamino acids 5.0 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l Na glutamate 1.0 g/l FeCl2 x 4 H2O 0.036 g/l MnCl2 x 4 H2O 0.00036 g/l Distilled water

culture temp

• @ref: 24291
• growth: positive
• type: growth
• temperature: 37
• range: mesophilic

Isolation, sampling and environmental information

isolation

• @ref: 24291
• sample type: saline cinders from a seawater splash zone at the top of a lava escarpment
• geographic location: Hawai'i Island
• country: USA
• origin.country: USA
• continent: North America

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_194106.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17828;96_71551;97_92980;98_126480;99_194106&stattab=map
• Last taxonomy: Haloferax
• 16S sequence: KT970727
• Sequence Identity:
• Total samples: 21
• soil counts: 7
• aquatic counts: 14

Safety information

risk assessment

• @ref: 24291
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 24291
• description: Haloferax namakaokahaiae strain Mke2.3 16S ribosomal RNA gene, partial sequence
• accession: KT970727
• length: 1264
• database: ena
• NCBI tax ID: 1748331

Genome sequences

• @ref: 66792
• description: Haloferax namakaokahaiae Mke2.3
• accession: 2756170193
• assembly level: draft
• database: img
• NCBI tax ID: 1748331

GC content

• @ref: 24291
• GC-content: 61.5
• method: high performance liquid chromatography (HPLC)

External links

@ref: 24291

culture collection no.: DSM 29988, LMG 29162

straininfo link

• @ref: 90828
• straininfo: 396487

literature

• topic: Phylogeny
• Pubmed-ID: 26906098
• title: Isolation and characterization of extremely halophilic CO-oxidizing Euryarchaeota from hypersaline cinders, sediments and soils and description of a novel CO oxidizer, Haloferax namakaokahaiae Mke2.3T, sp. nov.
• authors: McDuff S, King GM, Neupane S, Myers MR
• journal: FEMS Microbiol Ecol
• DOI: 10.1093/femsec/fiw028
• year: 2016
• mesh: Aerobiosis, Carbon Monoxide/*metabolism, Chile, DNA, Ribosomal/genetics, Genome, Archaeal/genetics, Geologic Sediments/microbiology, *Haloferax/genetics/isolation & purification/metabolism, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, *Salinity, Sodium Chloride/*metabolism, Soil/chemistry, *Soil Microbiology
• topic2: Metabolism

Reference