Strain identifier

BacDive ID: 132449

Type strain: Yes

Species: Oceanobacillus halophilus

Strain Designation: J8B

Strain history: <- M. A. Amoozegar, Univ. Tehran, Iran; J8B <- M. Bagheri

NCBI tax ID(s): 930130 (species)

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General

@ref: 24238

BacDive-ID: 132449

DSM-Number: 23996

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, Gram-positive, motile, rod-shaped, colony-forming

description: Oceanobacillus halophilus J8B is an obligate aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from brine of a hypersaline lake.

NCBI tax id

  • NCBI tax id: 930130
  • Matching level: species

strain history

  • @ref: 24238
  • history: <- M. A. Amoozegar, Univ. Tehran, Iran; J8B <- M. Bagheri

doi: 10.13145/bacdive132449.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Oceanobacillus
  • species: Oceanobacillus halophilus
  • full scientific name: Oceanobacillus halophilus Amoozegar et al. 2016

@ref: 24238

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Oceanobacillus

species: Oceanobacillus halophilus

full scientific name: Oceanobacillus halophilus Amoozegar et al. 2016

strain designation: J8B

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43737positive1.5-2 µm0.8-0.9 µmrod-shapedyes
69480yes98.017
69480positive100

colony morphology

  • @ref: 43737
  • colony size: 3.2 mm
  • colony color: Cream
  • colony shape: circular
  • incubation period: 2 days
  • medium used: HM medium

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43737HM mediumyes
24238BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 40.0 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43737positivegrowth20-45
43737positiveoptimum35mesophilic
24238positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
43737positivegrowth6.5-9alkaliphile
43737positiveoptimum8

Physiology and metabolism

oxygen tolerance

  • @ref: 43737
  • oxygen tolerance: obligate aerobe

spore formation

@refspore descriptiontype of sporespore formationconfidence
43737oval subterminal endospores in swollen sporangiaendosporeyes
69481yes99
69480yes99.999

halophily

@refsaltgrowthtested relationconcentrationhalophily level
43737NaClpositivegrowth3-15 %(w/v)
43737NaClpositiveoptimum7.5 %(w/v)moderately halophilic

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4373716947citrate-assimilation
4373715824D-fructose-builds acid from
4373716899D-mannitol-builds acid from
4373716899D-mannitol-carbon source
4373716024D-mannose-carbon source
4373717754glycerol-carbon source
4373715428glycine-carbon source
4373716977L-alanine-carbon source
4373730849L-arabinose-carbon source
4373716467L-arginine-carbon source
4373717196L-asparagine-carbon source
4373729991L-aspartate-carbon source
4373717561L-cysteine-carbon source
4373715971L-histidine-carbon source
4373716643L-methionine-carbon source
4373717295L-phenylalanine-carbon source
4373717203L-proline-carbon source
4373717895L-tyrosine-carbon source
4373716414L-valine-carbon source
4373728053melibiose-carbon source
4373716634raffinose-carbon source
4373727082trehalose-carbon source
4373717234glucose-fermentation
4373726710sodium chloride-growth
43737casein-hydrolysis
4373716991dna-hydrolysis
437374853esculin-hydrolysis
437375291gelatin-hydrolysis
4373728017starch-hydrolysis
4373753423tween 40-hydrolysis
4373753425tween 60-hydrolysis
4373753426tween 80-hydrolysis
4373716199urea-hydrolysis
4373716301nitrite-reduction
4373712936D-galactose+builds acid from
4373717634D-glucose+builds acid from
4373716988D-ribose+builds acid from
4373765327D-xylose+builds acid from
4373717716lactose+builds acid from
4373717306maltose+builds acid from
4373717992sucrose+builds acid from
4373717057cellobiose+carbon source
4373715824D-fructose+carbon source
4373712936D-galactose+carbon source
4373717634D-glucose+carbon source
4373716988D-ribose+carbon source
4373765327D-xylose+carbon source
4373717992sucrose+carbon source
4373717057cellobiose+energy source
4373715824D-fructose+energy source
4373712936D-galactose+energy source
4373717634D-glucose+energy source
4373716988D-ribose+energy source
4373765327D-xylose+energy source
4373717992sucrose+energy source
4373753424tween 20+hydrolysis
4373717632nitrate+reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
4373728971ampicillinyesyes10 µg (disc)
437373393carbenicillinyesyes100 µg (disc)
43737124991cefalotinyesyes30 µg (disc)
4373717698chloramphenicolyesyes30 µg (disc)
4373771415nitrofurantoinyesyes300 µg (disc)
4373717076streptomycinyesyes10 µg (disc)
4373727902tetracyclineyesyes30 µg (disc)
4373728864tobramycinyesyes10 µg (disc)
4373728669bacitracinyesyes10 Unit (disc)
4373718208penicillin gyesyes10 Unit (disc)
437372637amikacinyesyes30 µg (disc)
437372676amoxicillinyesyes25 µg (disc)
4373748923erythromycinyesyes5 µg (disc)
437376104kanamycinyesyes5 µg (disc)
4373717833gentamicinyesyes10 µg (disc)
43737100147nalidixic acidyesyes30 µg (disc)
4373728077rifampicinyesyes5 µg (disc)
437378309polymyxin byesyes100 Unit (disc)

metabolite production

@refChebi-IDmetaboliteproduction
4373715688acetoinno
4373716136hydrogen sulfideno
4373735581indoleno

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-test
4373717234glucose-
4373715688acetoin-

enzymes

@refvalueactivityec
43737catalase+1.11.1.6
43737cytochrome oxidase+1.9.3.1
43737ornithine decarboxylase-4.1.1.17
43737arginine dihydrolase-3.5.3.6
43737lysine decarboxylase-4.1.1.18

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43737C15:0 anteiso22.6
    43737C17:0 anteiso14.7
    43737C16:022.9
    43737C16:0 iso19.4
  • type of FA analysis: whole cell analysis
  • incubation medium: 7.5 % HM broth
  • agar/liquid: liquid
  • incubation temperature: 35
  • incubation_oxygen: aerobic
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA40 4.10
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
24238brine of a hypersaline lakeAran-Bidgol lake (34° 26' N 51° 48' E)IranIRNAsia
43737Brine sample of a hypersaline lakeAran-BidgolIranIRNAsia

isolation source categories

Cat1Cat2Cat3
#Condition#Saline
#Environmental#Aquatic#Lake (large)

taxonmaps

  • @ref: 69479
  • File name: preview.99_20886.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_8982;97_12824;98_15777;99_20886&stattab=map
  • Last taxonomy: Oceanobacillus halophilus subclade
  • 16S sequence: HQ433454
  • Sequence Identity:
  • Total samples: 19
  • soil counts: 8
  • aquatic counts: 7
  • animal counts: 4

Safety information

risk assessment

  • @ref: 24238
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24238
  • description: Oceanobacillus halophilus strain J8B 16S ribosomal RNA gene, partial sequence
  • accession: HQ433454
  • length: 1445
  • database: ena
  • NCBI tax ID: 930130

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Oceanobacillus halophilus DSM 23996GCA_003628505contigncbi930130
66792Oceanobacillus halophilus strain DSM 23996930130.3wgspatric930130
66792Oceanobacillus halophilus DSM 239962840770658draftimg930130

GC content

  • @ref: 24238
  • GC-content: 39.2
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes99no
motileyes90.019yes
flagellatedyes86.205no
gram-positiveyes88.253yes
anaerobicno97.309no
halophileyes91.725yes
spore-formingyes92.153no
thermophileno96.619yes
glucose-utilyes89.395yes
aerobicyes83.537yes
glucose-fermentno89.621yes

External links

@ref: 24238

culture collection no.: DSM 23996, IBRC-M 10444

straininfo link

  • @ref: 90787
  • straininfo: 398126

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny26869142Oceanobacillus halophilus sp. nov., a novel moderately halophilic bacterium from a hypersaline lake.Amoozegar MA, Bagheri M, Makhdoumi A, Nikou MM, Fazeli SAS, Schumann P, Sproer C, Sanchez-Porro C, Ventosa AInt J Syst Evol Microbiol10.1099/ijsem.0.0009522016
Phylogeny33763766Oceanobacillus salinisoli sp. nov., a bacterium isolated from saline soil of Turpan city in Xinjiang province, north-west China.Zhu WY, Yang L, Zhang ZT, Mu CG, Wang Y, Kou YR, Jiang GQ, Yin M, Tang SKArch Microbiol10.1007/s00203-021-02287-62021Bacillaceae/classification/*isolation & purification, Phylogeny, *Soil MicrobiologyEnzymology

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24238Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-23996Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23996)
43737Mohammad Ali Amoozegar, Maryam Bagheri, Ali Makhdoumi, Mahdi Moshtaghi Nikou, Seyed Abolhassan Shahzadeh Fazeli, Peter Schumann, Cathrin Spröer, Cristina Sánchez-Porro, Antonio VentosaOceanobacillus halophilus sp. nov., a novel moderately halophilic bacterium from a hypersaline lake10.1099/ijsem.0.000952IJSEM 66: 1317-1322 201626869142
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
90787Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID398126.1