Strain identifier

BacDive ID: 132373

Type strain: Yes

Species: Intrasporangium flavum

Strain history: <- L.-H. Lee, Monash Univ., Malaysia

NCBI tax ID(s): 1428657 (species)

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General

@ref: 24162

BacDive-ID: 132373

DSM-Number: 29621

keywords: 16S sequence, Bacteria, aerobe, Gram-positive, rod-shaped

description: Intrasporangium flavum DSM 29621 is an aerobe, Gram-positive, rod-shaped bacterium that was isolated from mangrove soil, top layer.

NCBI tax id

  • NCBI tax id: 1428657
  • Matching level: species

strain history

  • @ref: 24162
  • history: <- L.-H. Lee, Monash Univ., Malaysia

doi: 10.13145/bacdive132373.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Intrasporangiaceae
  • genus: Intrasporangium
  • species: Intrasporangium flavum
  • full scientific name: Intrasporangium flavum (Azman et al. 2016) Nouioui et al. 2018

  • synonyms

    • @ref: 20215
    • synonym: Monashia flava

@ref: 24162

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Micrococcales

family: Intrasporangiaceae

genus: Intrasporangium

species: Intrasporangium flavum

full scientific name: Intrasporangium flavum (Azman et al. 2016) Nouioui et al. 2018

type strain: yes

Morphology

cell morphology

  • @ref: 43803
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colormedium used
43803Yellowish-whiteISP 2
43803Yellowish-whiteAIA

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43803AIAyes
43803ISP 2yes
43803ISP 7yes
43803LB (Luria-Bertani) MEDIUMyes
43803Nutrient agar (NA)yes
43803R2A agaryes
43803SAyes
24162GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43803positivegrowth24-36
43803positiveoptimum28-32mesophilic
24162positivegrowth28mesophilic

culture pH

@refabilitytypepH
43803positivegrowth6-8
43803positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 43803
  • oxygen tolerance: aerobe

spore formation

  • @ref: 43803
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
43803NaClpositivegrowth0-4 %
43803NaClpositiveoptimum0-2 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4380316651(S)-lactate-carbon source
43803309162-oxoglutarate-carbon source
43803739183-O-methyl-D-glucose-carbon source
438038295beta-hydroxybutyrate-carbon source
4380329990D-aspartate-carbon source
4380328847D-fucose-carbon source
4380316899D-mannitol-carbon source
4380316523D-serine-carbon source
4380317754glycerol-carbon source
4380317464L-galactonic acid gamma-lactone-carbon source
4380328053melibiose-carbon source
4380317268myo-inositol-carbon source
4380317309pectin-carbon source
4380317272propionate-carbon source
4380335020tributyrin-hydrolysis
4380337166xylan-hydrolysis
4380329985L-glutamate-nitrogen source
4380315588D-malate+carbon source
4380315589L-malate+carbon source
43803167632-oxobutanoate+carbon source
43803181014-hydroxyphenylacetic acid+carbon source
4380313705acetoacetate+carbon source
4380317925alpha-D-glucose+carbon source
43803645522-hydroxybutyrate+carbon source
4380336219alpha-lactose+carbon source
4380373706bromosuccinate+carbon source
4380317057cellobiose+carbon source
4380316947citrate+carbon source
4380318333D-arabitol+carbon source
4380315824D-fructose+carbon source
4380378697D-fructose 6-phosphate+carbon source
4380312936D-galactose+carbon source
4380318024D-galacturonic acid+carbon source
438038391D-gluconate+carbon source
4380314314D-glucose 6-phosphate+carbon source
4380315748D-glucuronate+carbon source
4380316024D-mannose+carbon source
4380333801D-saccharate+carbon source
4380317924D-sorbitol+carbon source
4380323652dextrin+carbon source
4380315740formate+carbon source
4380316537galactarate+carbon source
4380316865gamma-aminobutyric acid+carbon source
438035291gelatin+carbon source
4380328066gentiobiose+carbon source
4380332323glucuronamide+carbon source
4380370744glycine-proline+carbon source
4380316411indole-3-acetic acid+carbon source
4380317596inosine+carbon source
4380318287L-fucose+carbon source
4380362345L-rhamnose+carbon source
4380317306maltose+carbon source
4380374611methyl (R)-lactate+carbon source
4380337657methyl D-glucoside+carbon source
4380351850methyl pyruvate+carbon source
4380363154N-acetyl-beta-D-mannosamine+carbon source
4380328037N-acetylgalactosamine+carbon source
43803506227N-acetylglucosamine+carbon source
4380335418n-acetylneuraminate+carbon source
4380326490quinate+carbon source
4380316634raffinose+carbon source
4380317814salicin+carbon source
4380317164stachyose+carbon source
4380317992sucrose+carbon source
4380327082trehalose+carbon source
4380332528turanose+carbon source
4380353423tween 40+carbon source
4380385146carboxymethylcellulose+hydrolysis
43803casein+hydrolysis
4380317029chitin+hydrolysis
4380328017starch+hydrolysis
4380316977L-alanine+nitrogen source
4380316467L-arginine+nitrogen source
4380329991L-aspartate+nitrogen source
4380315971L-histidine+nitrogen source
4380318183L-pyroglutamic acid+nitrogen source
4380317115L-serine+nitrogen source

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.group IDis resistantresistance conc.
4380328971ampicillinyesyes10 µg (disc)
4380328971ampicillinyesyes30 µg (disc)20
43803sulbactamyesyes30 µg (disc)20
43803204928cefotaximeyesyes30 µg (disc)
438033515cefuroximeyesyes30 µg (disc)
4380323066cephalosporinyesyes30 µg (disc)
4380317698chloramphenicolyesyes30 µg (disc)
43803100241ciprofloxacinyesyes10 µg (disc)
4380348923erythromycinyesyes15 µg (disc)
4380317833gentamicinyesyes20 µg (disc)
4380318208penicillin gyesyes10 µg (disc)
4380317076streptomycinyesyes10 µg (disc)
4380327902tetracyclineyesyes30 µg (disc)
4380328001vancomycinyesyes30 µg (disc)
4380371321fusidateyesyes
4380316523d-serineyesyes
4380345735troleandomycinyesyes
4380350694minocyclineyesyes
438036472lincomycinyesyes
4380332735guanidinium chlorideyesyes
4380375273niaproofyesyes
4380375193tetrazolium violetyesyes
4380375198tetrazolium blueyesyes
4380375229sodium bromateyesyes
438031 % sodium lactateyesyes
4380328077rifampicinyesyes
4380348607lithium chlorideyesyes
4380375248potassium telluriteyesyes
43803161680aztreonamyesyes
4380364103sodium butyrateyesyes
43803100147nalidixic acidyesyes30 µg (disc)

enzymes

  • @ref: 43803
  • value: catalase
  • activity: +
  • ec: 1.11.1.6

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeisolation date
24162mangrove soil, top layerPahang State, Kuantan, Tanjung Lumpur (3° 48' 3.2'' N 103° 20' 11.0'' E)MalaysiaMYSAsia3.8009103.336
43803Soil from the mangrove forests of the Tanjung Lumpur in the city KuantanKuantan, PahangMalaysiaMYSAsia3.8103.3362012-12

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Mangrove
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil

Safety information

risk assessment

  • @ref: 24162
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24162
  • description: Monashia flava strain MUSC 78 16S ribosomal RNA gene, partial sequence
  • accession: KF682157
  • length: 1488
  • database: ena
  • NCBI tax ID: 1428657

GC content

  • @ref: 24162
  • GC-content: 73.1
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 24162

culture collection no.: DSM 29621, MCCC 1K00454, NBRC 110749, MUSC 78

straininfo link

  • @ref: 90717
  • straininfo: 404781

literature

topicPubmed-IDtitleauthorsjournalDOIyear
Phylogeny26556816Monashia flava gen. nov., sp. nov., an actinobacterium of the family Intrasporangiaceae.Azman AS, Zainal N, Mutalib NA, Yin WF, Chan KG, Lee LHInt J Syst Evol Microbiol10.1099/ijsem.0.0007532015
28611495Antibacterial, Anticancer and Neuroprotective Activities of Rare Actinobacteria from Mangrove Forest Soils.Azman AS, Othman I, Fang CM, Chan KG, Goh BH, Lee LHIndian J Microbiol10.1007/s12088-016-0627-z2016

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24162Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29621Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29621)
43803Adzzie-Shazleen Azman, Nurullhudda Zainal, Nurul-Syakima Ab Mutalib, Wai-Fong Yin, Kok-Gan Chan, Learn-Han LeeMonashia flava gen. nov., sp. nov., an actinobacterium of the family Intrasporangiaceae10.1099/ijsem.0.000753IJSEM 66: 554-561 201626556816
90717Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID404781.1