Strain identifier

BacDive ID: 132370

Type strain: Yes

Species: Serratia aquatilis

Strain Designation: 2015-2462-01

Strain history: CIP <- 2015, P. Kämpfer, Giessen Univ., Giessen, Germany: strain 2015-2462-01

NCBI tax ID(s): 1737515 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 24159

BacDive-ID: 132370

DSM-Number: 100980

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Serratia aquatilis 2015-2462-01 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from drinking water distribution system.

NCBI tax id

  • NCBI tax id: 1737515
  • Matching level: species

strain history

@refhistory
24159<- P. Kämpfer, Justus-Liebig-Universität, Giessen, Germany; 2015-2462-01
116447CIP <- 2015, P. Kämpfer, Giessen Univ., Giessen, Germany: strain 2015-2462-01

doi: 10.13145/bacdive132370.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Yersiniaceae
  • genus: Serratia
  • species: Serratia aquatilis
  • full scientific name: Serratia aquatilis Kämpfer and Glaeser 2016

@ref: 24159

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Yersiniaceae

genus: Serratia

species: Serratia aquatilis

full scientific name: Serratia aquatilis Kämpfer and Glaeser 2016

strain designation: 2015-2462-01

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
65210negative2 µm1 µmrod-shapedyes
116447negativerod-shapedno

colony morphology

  • @ref: 65210
  • colony size: 5 mm
  • colony color: cream-white
  • medium used: nutrient agar

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
42183MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
24159TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yesName: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/535
65210Nutrient agar (NA)yes
65210brain heart infusion agaryes
65210trypticase soy agaryes
65210Reasoner's 2A agar (R2A)yes
65210MacConkey agaryes
116447CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
42183positivegrowth37mesophilic
24159positivegrowth28mesophilic
65210positivegrowth4-45
65210nogrowth50thermophilic
65210nogrowth<4psychrophilic

culture pH

  • @ref: 65210
  • ability: positive
  • type: growth
  • pH: 5.5-9.5
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 65210
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 65210
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
65210NaClpositivegrowth0-6 %(w/v)
65210NaClnogrowth4 %(w/v)

observation

@refobservation
65210production of brown diffusible pigments on tyrosine agar
65210Acid production from lactose is weak and occurs only after prolonged incubation

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
652102-deoxythymidine-5'-4-nitrophenyl phosphate+hydrolysis
652102-oxoglutarate-carbon source30916
652103-hydroxybenzoate-carbon source16193
65210gamma-aminobutyric acid-carbon source16865
652104-hydroxybenzoate-carbon source17879
652104-nitrophenyl alpha-D-glucopyranoside-hydrolysis91122
652104-nitrophenyl beta-D-galactopyranoside+hydrolysis355715
652104-nitrophenyl beta-D-glucopyranoside+hydrolysis90259
652104-nitrophenyl beta-D-glucuronide-hydrolysis90146
652104-nitrophenyl beta-D-xylopyranoside+hydrolysis90148
65210acetate+carbon source30089
65210adipate-carbon source17128
65210ribitol+builds acid from15963
65210ribitol+carbon source15963
65210ammonium+nitrogen source28938
65210arbutin+assimilation18305
65210arbutin+carbon source18305
65210azelaate-carbon source78208
65210beta-alanine-carbon source16958
65210bis-4-nitrophenyl phosphate+hydrolysis3122
65210bis-4-nitrophenyl-phenyl phosphonate+hydrolysis91050
65210bis-4-nitrophenyl-phosphorylcholine-hydrolysis55394
65210cellobiose+assimilation17057
65210cellobiose+builds acid from17057
65210cellobiose+carbon source17057
65210cis-aconitate+carbon source16383
65210citrate+assimilation16947
65210citrate+carbon source16947
65210D-arabitol+builds acid from18333
65210D-fructose+assimilation15824
65210D-fructose+carbon source15824
65210D-galactose-assimilation12936
65210D-galactose-carbon source12936
65210D-glucose+assimilation17634
65210D-glucose+builds acid from17634
65210D-glucose+carbon source17634
65210D-mannitol+assimilation16899
65210D-mannitol+builds acid from16899
65210D-mannitol+carbon source16899
65210D-mannose+assimilation16024
65210D-mannose+builds acid from16024
65210D-mannose+carbon source16024
65210D-ribose+carbon source16988
65210D-sorbitol+assimilation17924
65210D-sorbitol+builds acid from17924
65210D-sorbitol+carbon source17924
65210D-xylose+assimilation65327
65210D-xylose+builds acid from65327
65210D-xylose+carbon source65327
652103-hydroxybutyrate-carbon source37054
65210lactate+carbon source24996
65210galactitol-builds acid from16813
65210erythritol-assimilation17113
65210erythritol+builds acid from17113
65210esculin+assimilation4853
65210esculin+hydrolysis4853
65210fumarate+carbon source29806
65210gamma-L-glutamate-4-nitroanilide+hydrolysis
65210gluconate+carbon source24265
65210glutarate-carbon source30921
65210glycerol+carbon source17754
65210glycogen-assimilation28087
65210myo-inositol+assimilation17268
65210itaconate-carbon source17240
65210L-alanine+carbon source16977
65210L-alanine 4-nitroanilide+hydrolysis
65210L-arabinose+assimilation30849
65210L-arabinose+builds acid from30849
65210L-arabinose+carbon source30849
65210L-arginine-assimilation16467
65210L-aspartate+carbon source29991
65210L-histidine+carbon source15971
65210L-leucine-carbon source15603
65210L-ornithine-assimilation15729
65210L-ornithine-carbon source15729
65210L-phenylalanine-carbon source17295
65210L-proline-carbon source17203
65210L-proline-4-nitroanilide+hydrolysis
65210L-rhamnose-assimilation62345
65210L-rhamnose-carbon source62345
65210L-rhamnose+builds acid from62345
65210L-serine+carbon source17115
65210L-sorbose-assimilation17266
65210L-tryptophan-assimilation16828
65210L-tryptophan-carbon source16828
65210lactose+builds acid from17716
65210malonate+builds base from15792
65210maltitol-carbon source68428
65210maltose+assimilation17306
65210maltose+builds acid from17306
65210maltose+carbon source17306
65210melibiose+assimilation28053
65210melibiose+builds acid from28053
65210melibiose+carbon source28053
65210mesaconate+/-carbon source36986
65210myo-inositol+builds acid from17268
65210myo-inositol+carbon source17268
65210methyl alpha-D-glucopyranoside+builds acid from320061
65210N-acetylgalactosamine+carbon source28037
65210N-acetylglucosamine+carbon source506227
65210phenylacetate-carbon source18401
65210propionate-carbon source17272
65210putrescine-carbon source17148
65210pyruvate+assimilation15361
65210pyruvate+carbon source15361
65210raffinose+builds acid from16634
65210salicin+builds acid from17814
65210salicin+carbon source17814
65210sodium thiosulfate-assimilation132112
65210suberic acid-carbon source9300
65210sucrose+assimilation17992
65210sucrose+builds acid from17992
65210sucrose+carbon source17992
65210trans-aconitate+carbon source15708
65210trehalose+assimilation27082
65210trehalose+builds acid from27082
65210trehalose+carbon source27082
68368L-arabinose+fermentation30849
68368amygdalin+fermentation27613
68368melibiose+fermentation28053
68368sucrose+fermentation17992
68368L-rhamnose+fermentation62345
68368sorbitol+fermentation30911
68368myo-inositol+fermentation17268
68368D-mannitol+fermentation16899
68368D-glucose+fermentation17634
68368gelatin-hydrolysis5291
68368tryptophan-energy source27897
68368urea+hydrolysis16199
68368citrate+assimilation16947
68368ornithine-degradation18257
68368lysine-degradation25094
68368arginine-hydrolysis29016

metabolite production

@refChebi-IDmetaboliteproduction
6521015688acetoinno
6521016136hydrogen sulfideno
6521035581indoleno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitecitrate testindole test
6521016947citrate+
6836835581indole-

enzymes

@refvalueactivityec
65210cytochrome oxidase-1.9.3.1
65210arginine dihydrolase-3.5.3.6
65210ornithine decarboxylase-4.1.1.17
65210lysine decarboxylase-4.1.1.18
65210urease+3.5.1.5
65210beta-galactosidase+3.2.1.23
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65210C14:07.9
    65210C15:03.3
    65210C14:0 3OH / C16:1 iso I7.9
    65210C16:1ω7c33.9
    65210C16:029.6
    65210C17:0 cyclo4
    65210C17:02.2
  • type of FA analysis: whole cell analysis
  • system: MIS MIDI
  • method/protocol: Kämpfer & Kroppenstedt 1996

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
24159+---+-+----+++++++++-
24159+---+-+--+-+++++++++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
24159drinking water distribution systemRhine–Ruhr areaGermanyDEUEurope
65210drinking water systemGermanyDEUEurope
116447Environment, Water distribution systemGermanyDEUEurope2015-01-02

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Freshwater

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
241591Risk group (German classification)
1164471Risk group (French classification)

Sequence information

16S sequences

  • @ref: 24159
  • description: Serratia aquatilis 16S ribosomal RNA gene, partial sequence
  • accession: KT387999
  • length: 1454
  • database: ena
  • NCBI tax ID: 1737515

External links

@ref: 24159

culture collection no.: DSM 100980, CCM 8626, LMG 29119, CIP 110919

straininfo link

  • @ref: 90714
  • straininfo: 396480

literature

  • topic: Phylogeny
  • Pubmed-ID: 26537514
  • title: Serratia aquatilis sp. nov., isolated from drinking water systems.
  • authors: Kampfer P, Glaeser SP
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000731
  • year: 2015
  • mesh: Bacterial Typing Techniques, DNA, Bacterial/genetics, Drinking Water/*microbiology, Fatty Acids/chemistry, Genes, Bacterial, Germany, Molecular Sequence Data, Multilocus Sequence Typing, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Serratia/*classification/genetics/isolation & purification
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24159Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100980Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100980)
42183Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/34526
65210Peter Kämpfer, Stefanie P. GlaeserSerratia aquatilis sp. nov., isolated from drinking water systems10.1099/ijsem.0.000731IJSEM 66: 407-413 201626537514
68368Automatically annotated from API 20E
90714Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID396480.1
116447Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110919Collection of Institut Pasteur (CIP 110919)