Strain identifier

BacDive ID: 132357

Type strain: Yes

Species: Acidithiobacillus ferriphilus

Strain Designation: M20

Strain history: C. Falagán; Bangor Univ., UK; M20.

NCBI tax ID(s): 1689834 (species)

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General

@ref: 24146

BacDive-ID: 132357

DSM-Number: 100412

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, chemolithotroph, mesophilic, Gram-negative, motile, rod-shaped

description: Acidithiobacillus ferriphilus M20 is a facultative anaerobe, chemolithotroph, mesophilic bacterium that was isolated from acidic pool in a geothermal area.

NCBI tax id

  • NCBI tax id: 1689834
  • Matching level: species

strain history

@refhistory
24146<- C. Falagan, Bangor Univ., Wales, U.K.; M20 <- B. Johnson {1996}
67770C. Falagán; Bangor Univ., UK; M20.

doi: 10.13145/bacdive132357.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Acidithiobacillia
  • order: Acidithiobacillales
  • family: Acidithiobacillaceae
  • genus: Acidithiobacillus
  • species: Acidithiobacillus ferriphilus
  • full scientific name: Acidithiobacillus ferriphilus Falagán and Johnson 2016

@ref: 24146

domain: Bacteria

phylum: Proteobacteria

class: Acidithiobacillia

order: Acidithiobacillales

family: Acidithiobacillaceae

genus: Acidithiobacillus

species: Acidithiobacillus ferriphilus

full scientific name: Acidithiobacillus ferriphilus Falagán and Johnson 2016

strain designation: M20

type strain: yes

Morphology

cell morphology

  • @ref: 43830
  • gram stain: negative
  • cell length: 1-2 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 43830
  • colony color: Ferric-iron stained
  • medium used: Acidic ferrous iron overlay media

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43830Acidic ferrous iron overlay mediayes
43830YEno
24146ACIDITHIOBACILLUS (APH) MEDIUM (DSMZ Medium 271)yeshttps://mediadive.dsmz.de/medium/271Name: ACIDITHIOBACILLUS (APH) MEDIUM (DSMZ Medium 271) Composition: FeSO4 x 7 H2O 8.0 g/l (NH4)2SO4 2.0 g/l MgSO4 x 7 H2O 0.5 g/l K2HPO4 0.5 g/l KCl 0.1 g/l Ca(NO3)2 x 4 H2O 0.02 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43830positiveoptimum30mesophilic
24146positivegrowth30mesophilic
67770positivegrowth30mesophilic

culture pH

  • @ref: 43830
  • ability: positive
  • type: optimum
  • pH: 2
  • PH range: acidophile

Physiology and metabolism

oxygen tolerance

  • @ref: 43830
  • oxygen tolerance: facultative anaerobe

nutrition type

  • @ref: 43830
  • type: chemolithotroph

spore formation

  • @ref: 43830
  • type of spore: endospore
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4383018276dihydrogen-growth
4383017754glycerol-growth
4383033403elemental sulfur+electron donor
4383029034ferric iron+electron donor
4383015226tetrathionate+electron donor

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
24146acidic pool in a geothermal areaGalway’s Soufriere (16° 40' 06'' N 62° 10' 95''W)Montserrat (British Overseas Territory)GBRMiddle and South America16.6683-62.1931
43830An acidic pool in a geothermal area of MontserratMontserrat
67770Stream at Galway's soufriereisland of MontserratUnited KingdomGBREurope

isolation source categories

Cat1Cat2
#Condition#Acidic
#Environmental#Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_1491.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_163;96_544;97_628;98_885;99_1491&stattab=map
  • Last taxonomy: Acidithiobacillus
  • 16S sequence: KR905751
  • Sequence Identity:
  • Total samples: 646
  • soil counts: 127
  • aquatic counts: 432
  • animal counts: 60
  • plant counts: 27

Safety information

risk assessment

  • @ref: 24146
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24146
  • description: Acidithiobacillus ferriphilus strain M20 16S ribosomal RNA gene, partial sequence
  • accession: KR905751
  • length: 1323
  • database: ena
  • NCBI tax ID: 1689834

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acidithiobacillus ferriphilus DSM 100412GCA_018854775contigncbi1689834
66792Acidithiobacillus ferriphilus strain DSM 1004121689834.8wgspatric1689834

GC content

@refGC-contentmethod
4383057.4
2414657.4high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes88.36yes
gram-positiveno98.025yes
anaerobicno97.389yes
aerobicno71.312no
halophileno91.03no
spore-formingno93.571yes
glucose-utilyes53.742no
flagellatedno79.717no
thermophileno88.623yes
glucose-fermentno84.531no

External links

@ref: 24146

culture collection no.: DSM 100412, JCM 30830

straininfo link

  • @ref: 90703
  • straininfo: 404003

literature

  • topic: Phylogeny
  • Pubmed-ID: 26498321
  • title: Acidithiobacillus ferriphilus sp. nov., a facultatively anaerobic iron- and sulfur-metabolizing extreme acidophile.
  • authors: Falagan C, Johnson DB
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000698
  • year: 2015
  • mesh: Acidithiobacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hydrogen-Ion Concentration, Iron/*metabolism, Molecular Sequence Data, Oxidation-Reduction, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sulfides/*metabolism, Sulfur/*metabolism, Sulfur-Reducing Bacteria/classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry, *Water Microbiology
  • topic2: Metabolism

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24146Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100412Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100412)
43830Carmen Falagán, D. Barrie JohnsonAcidithiobacillus ferriphilus sp. nov., a facultatively anaerobic iron- and sulfur-metabolizing extreme acidophile10.1099/ijsem.0.000698IJSEM 66: 206-211 201626498321
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
90703Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID404003.1