General

@ref: 20651

BacDive-ID: 132151

DSM-Number: 24973

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped

description: Methylomonas paludis MG30 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from acidic Sphagnum peat bog.

NCBI tax id

• NCBI tax id: 1173101
• Matching level: species

strain history

• @ref: 20651
• history: <- S. N. Dedysh, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; MG30 <- I. S. Kulichevskaya

doi: 10.13145/bacdive132151.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/pseudomonadota
• domain: Bacteria
• phylum: Pseudomonadota
• class: Gammaproteobacteria
• order: Methylococcales
• family: Methylococcaceae
• genus: Methylomonas
• species: Methylomonas paludis
• full scientific name: Methylomonas paludis Danilova et al. 2013

@ref: 20651

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Methylococcales

family: Methylococcaceae

genus: Methylomonas

species: Methylomonas paludis

full scientific name: Methylomonas paludis Danilova et al. 2013

strain designation: MG30

type strain: yes

Morphology

cell morphology

• @ref: 30839
• gram stain: negative
• cell length: 2.5 µm
• cell width: 1.25 µm
• cell shape: rod-shaped
• motility: no

pigmentation

• @ref: 30839
• production: yes

Culture and growth conditions

culture medium

• @ref: 20651
• name: LM-MEDIUM (DSMZ Medium 1347)
• growth: yes
• link: https://mediadive.dsmz.de/medium/1347
• composition: Name: M2-MEDIUM (DSMZ Medium 1347) Composition: KNO3 0.24975 g/l KH2PO4 0.0999001 g/l MgSO4 x 7 H2O 0.04995 g/l NaCl 0.01998 g/l CaCl2 x 2 H2O 0.00999001 g/l EDTA 0.00499501 g/l FeSO4 x 7 H2O 0.001998 g/l CoCl2 x 6 H2O 0.0001998 g/l CuCl2 x 5 H2O 9.99001e-05 g/l ZnSO4 x 7 H2O 9.99001e-05 g/l Na2MoO4 2.997e-05 g/l NiCl2 x 6 H2O 1.998e-05 g/l MnCl2 x 4 H2O Distilled water

culture temp

culture pH

Physiology and metabolism

oxygen tolerance

• @ref: 30839
• oxygen tolerance: aerobe

halophily

• @ref: 30839
• salt: NaCl
• growth: positive
• tested relation: growth
• concentration: 0-0.1 %

Isolation, sampling and environmental information

isolation

• @ref: 20651
• sample type: acidic Sphagnum peat bog
• host species: Sphagnum
• geographic location: Karelia, island Valaam, bank of the bog lake Germanovskoe (61° 22' N 31° 07' E)
• country: Russia
• origin.country: RUS
• continent: Europe
• latitude: 61.3667
• longitude: 31.1167

isolation source categories

Safety information

risk assessment

• @ref: 20651
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 20651
• description: Methylomonas paludis partial 16S rRNA gene, type strain DSM 24973T
• accession: HE801216
• length: 1435
• database: ena
• NCBI tax ID: 1173101

GC content

External links

@ref: 20651

culture collection no.: DSM 24973, VKM B-2745

straininfo link

• @ref: 90507
• straininfo: 406151

literature

• topic: Phylogeny
• Pubmed-ID: 23159751
• title: Methylomonas paludis sp. nov., the first acid-tolerant member of the genus Methylomonas, from an acidic wetland.
• authors: Danilova OV, Kulichevskaya IS, Rozova ON, Detkova EN, Bodelier PLE, Trotsenko YA, Dedysh SN
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijs.0.045658-0
• year: 2012
• mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Methane/metabolism, Methanol/metabolism, Methylomonas/*classification/enzymology/genetics/isolation & purification, Molecular Sequence Data, Oxygenases/genetics, *Phylogeny, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, Sphagnopsida/*microbiology, *Wetlands
• topic2: Metabolism

Reference