Strain identifier
BacDive ID: 131904
Type strain: 
Species: Pseudomonas sp.
Strain Designation: Leaf83
Strain history: <- J. A. Vorholt, Institute of Microbiology, ETH Zurich, Switzerland; Leaf83 <- D. B. Müller, E. Potthoff and M. Remus-Emsermann
NCBI tax ID(s): 306 (species)
version 9.1 (current version)
General
@ref: 23696
BacDive-ID: 131904
DSM-Number: 102685
keywords: genome sequence, Bacteria, Gram-negative
description: Pseudomonas sp. Leaf83 is a Gram-negative bacterium that was isolated from leaf of Arabidopsis thaliana, wild-type genotype.
NCBI tax id
- NCBI tax id: 306
- Matching level: species
strain history
- @ref: 23696
- history: <- J. A. Vorholt, Institute of Microbiology, ETH Zurich, Switzerland; Leaf83 <- D. B. Müller, E. Potthoff and M. Remus-Emsermann
doi: 10.13145/bacdive131904.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas sp.
- full scientific name: Pseudomonas Migula 1894 (Approved Lists 1980)
synonyms
@ref synonym 20215 Serpens 20215 Flavimonas 20215 Stutzerimonas
@ref: 23696
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas sp.
full scientific name: Pseudomonas sp.
strain designation: Leaf83
type strain: no
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99
Culture and growth conditions
culture medium
- @ref: 23696
- name: NUTRIENT AGAR (DSMZ Medium 1)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1
- composition: Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
culture temp
- @ref: 23696
- growth: positive
- type: growth
- temperature: 28
Physiology and metabolism
oxygen tolerance
- @ref: 69480
- oxygen tolerance: aerobe
- confidence: 90.46
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | - | assimilation |
| 68369 | 27689 | decanoate | + | assimilation |
| 68369 | 24265 | gluconate | + | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 59640 | N-acetylglucosamine | - | assimilation |
| 68369 | 16899 | D-mannitol | + | assimilation |
| 68369 | 16024 | D-mannose | - | assimilation |
| 68369 | 30849 | L-arabinose | - | assimilation |
| 68369 | 17634 | D-glucose | + | assimilation |
| 68369 | 5291 | gelatin | - | hydrolysis |
| 68369 | 4853 | esculin | - | hydrolysis |
| 68369 | 16199 | urea | - | hydrolysis |
| 68369 | 29016 | arginine | - | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | + | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | - | |
| 68369 | beta-glucosidase | - | 3.2.1.21 |
| 68369 | urease | - | 3.5.1.5 |
| 68369 | arginine dihydrolase | - | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 23696 | + | - | - | - | - | - | - | - | + | - | - | + | - | - | + | + | - | + | + | - | + |
| 23696 | + | - | - | - | - | - | - | - | + | - | - | + | - | - | + | + | - | + | + | - | + |
Isolation, sampling and environmental information
isolation
- @ref: 23696
- sample type: leaf of Arabidopsis thaliana, wild-type genotype
- host species: Arabidopsis thaliana
- geographic location: Brugg (47.4816806N 8.217547222E)
- country: Switzerland
- origin.country: CHE
- continent: Europe
- latitude: 47.4817
- longitude: 8.2176
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Plants | #Herbaceous plants (Grass,Crops) |
| #Host Body-Site | #Plant | #Leaf (Phyllosphere) |
Sequence information
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Pseudomonas sp. Leaf83 | GCA_001422075 | scaffold | ncbi | 1736239 |
| 66792 | Pseudomonas sp. Leaf83 | 1736239.3 | wgs | patric | 1736239 |
| 66792 | Pseudomonas sp. Leaf83 | 2643221791 | draft | img | 1736239 |
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 99 | no |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 98.246 | no |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 90.46 | no |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 89.139 | no |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 100 | yes |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | yes | 89.973 | no |
External links
@ref: 23696
culture collection no.: DSM 102685
straininfo link
- @ref: 90291
- straininfo: 400800
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 23696 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-102685 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 102685) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 68369 | Automatically annotated from API 20NE | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 90291 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID400800.1 |