Archive BacDiveID:10.13145/bacdive1317.20230509.8.1

Strain identifier

BacDive ID: 1317

Type strain:

Species: Alteribacillus iranensis

Strain Designation: X5B

Strain history: <- M. A. Amoozegar, Univ. Tehran, Iran; X5 <- M. Bagheri

NCBI tax ID(s): 930128 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 17472

BacDive-ID: 1317

DSM-Number: 23995

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, motile, rod-shaped

description: Alteribacillus iranensis X5B is an aerobe, spore-forming, mesophilic bacterium that was isolated from mud of a hypersaline lake.

NCBI tax id

NCBI tax id: 930128
Matching level: species

strain history

@ref: 17472
history: <- M. A. Amoozegar, Univ. Tehran, Iran; X5 <- M. Bagheri

doi: 10.13145/bacdive1317.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/bacillota
domain: Bacteria
phylum: Bacillota
class: Bacilli
order: Caryophanales
family: Bacillaceae
genus: Alteribacillus
species: Alteribacillus iranensis
full scientific name: Alteribacillus iranensis (Bagheri et al. 2012) Azmatunnisa Begum et al. 2016
synonyms
- @ref: 20215
- synonym: Bacillus iranensis

@ref: 17472

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Alteribacillus

species: Alteribacillus iranensis

full scientific name: Alteribacillus iranensis (Bagheri et al. 2012) Azmatunnisa Begum et al. 2016

strain designation: X5B

type strain: yes

Morphology

cell morphology

@ref	cell length	cell width	cell shape	motility	gram stain	confidence
25003	1.2-2.5 µm	0.5-0.9 µm	rod-shaped	yes
30213	1.2-2.5 µm	0.5-0.9 µm	rod-shaped	yes	positive
69480				yes		96.346
69480					positive	100

pigmentation

@ref: 30213
production: yes

Culture and growth conditions

culture medium

@ref: 17472
name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
growth: yes
link: https://mediadive.dsmz.de/medium/514
composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 40.0 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

culture temp

@ref	growth	type	temperature	range
17472	positive	growth	30	mesophilic
25003	positive	growth	25-45
25003	positive	optimum	35	mesophilic
30213	positive	growth	25-45
30213	positive	optimum	35	mesophilic

culture pH

@ref	ability	type	pH	PH range
25003	positive	growth	7.0-10.0	alkaliphile
25003	positive	optimum	7.5
30213	positive	growth	07-10
30213	positive	optimum	7.5

Physiology and metabolism

oxygen tolerance

@ref: 30213
oxygen tolerance: aerobe

spore formation

@ref	type of spore	spore formation	confidence
25003	endospore	yes
30213		yes
69481		yes	100
69480		yes	100

halophily

@ref	salt	growth	tested relation	concentration
25003	NaCl	positive	growth	2.5-15.0 %(w/v)
25003	NaCl		optimum	5.0-7.5 %(w/v)
30213	NaCl	positive	growth	2.5-15 %
30213	NaCl	positive	optimum	5-7.5 %

metabolite utilization

@ref	Chebi-ID	metabolite	utilization activity	kind of utilization tested
25003	16651	(S)-lactate	-	carbon source
25003		casein	-	hydrolysis
25003	17108	D-arabinose	-	carbon source
25003	15824	D-fructose	-	builds acid from
25003	12936	D-galactose	-	builds gas from
25003	12936	D-galactose	-	builds acid from
25003	16899	D-mannitol	-	carbon source
25003	17924	D-sorbitol	-	builds acid from
25003	65327	D-xylose	-	builds gas from
25003	65327	D-xylose	-	builds acid from
25003	17234	glucose	-	builds acid from
25003	17754	glycerol	-	carbon source
25003	17716	lactose	-	builds gas from
25003	17716	lactose	-	builds acid from
25003	17306	maltose	-	builds acid from
25003	16811	methionine	-	carbon source
25003	16634	raffinose	-	builds acid from
25003	17814	salicin	-	builds gas from
25003	17814	salicin	-	builds acid from
25003	28017	starch	-	hydrolysis
25003	17992	sucrose	-	builds gas from
25003	17992	sucrose	-	builds acid from
25003	27082	trehalose	-	builds acid from
25003	53426	tween 80	-	hydrolysis
25003	17057	cellobiose	+	carbon source
25003	16899	D-mannitol	+	carbon source
25003	16899	D-mannitol	+	builds acid from
25003	17632	nitrate	+	reduction
30213	16449	alanine	+	carbon source
30213	17057	cellobiose	+	carbon source
30213	28757	fructose	+	carbon source
30213	17632	nitrate	+	reduction

enzymes

@ref	value	activity	ec
30213	catalase	+	1.11.1.6
30213	cytochrome oxidase	+	1.9.3.1

Isolation, sampling and environmental information

isolation

@ref: 17472
sample type: mud of a hypersaline lake
geographic location: Lake Aran-Bidgol (34.64378° N 51.83838° E)
country: Iran
origin.country: IRN
continent: Asia
latitude: 34.6438
longitude: 51.8384

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Terrestrial	#Mud (Sludge)
#Condition	#Saline

taxonmaps

@ref: 69479
File name: preview.99_20884.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_605;96_10605;97_12823;98_15775;99_20884&stattab=map
Last taxonomy: Alteribacillus iranensis subclade
16S sequence: HQ433452
Sequence Identity:
Total samples: 22
soil counts: 5
aquatic counts: 10
animal counts: 6
plant counts: 1

Safety information

risk assessment

@ref: 17472
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 17472
description: Alteribacillus iranensis strain X5B 16S ribosomal RNA gene, partial sequence
accession: HQ433452
length: 1439
database: ena
NCBI tax ID: 930128

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Alteribacillus iranensis DSM 23995	GCA_900113025	scaffold	ncbi	930128
66792	Alteribacillus iranensis strain DSM 23995	930128.3	wgs	patric	930128
66792	Alteribacillus iranensis DSM 23995	2651870176	draft	img	930128

GC content

@ref	GC-content	method
17472	42.4	high performance liquid chromatography (HPLC)
25003	41
30213	42.4
17472	41.0	high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

trait	prediction	confidence	training_data
spore-forming	yes	100	no
motile	yes	87.474	yes
flagellated	no	53.624	no
gram-positive	yes	85.786	no
anaerobic	no	95.213	no
aerobic	yes	90.493	yes
halophile	yes	84.452	no
spore-forming	yes	91.964	no
glucose-ferment	no	96.108	no
thermophile	no	98.075	yes
glucose-util	yes	74.551	no

External links

@ref: 17472

culture collection no.: DSM 23995, IBRC 10446

straininfo link

@ref: 70967
straininfo: 406675

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Phylogeny	21571930	Bacillus iranensis sp. nov., a moderate halophile from a hypersaline lake.	Bagheri M, Didari M, Amoozegar MA, Schumann P, Sanchez-Porro C, Mehrshad M, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijs.0.030874-0	2011	Bacillus/classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Geologic Sediments/microbiology, Iran, Lakes/microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Sodium Chloride, Spores, Bacterial/genetics, Vitamin K 2/chemistry, *Water Microbiology	Genetics
Phylogeny	26674773	Bacillus piscicola sp. nov., isolated from Thai fish sauce (Nam-pla).	Daroonpunt R, Itoh T, Kudo T, Ohkuma M, Tanasupawat S	Int J Syst Evol Microbiol	10.1099/ijsem.0.000851	2015
Phylogeny	30028282	Thalassorhabdus alkalitolerans gen. nov., sp. nov., a novel Bacillaceae member isolated from marine sediment.	Sultanpuram VR, Mothe T	Int J Syst Evol Microbiol	10.1099/ijsem.0.002931	2018	Bacillaceae/classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Geologic Sediments/microbiology, India, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phosphatidylglycerols/analysis, Phospholipids/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry	Transcriptome

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed	ID_cross_reference
17472	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23995)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-23995
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
25003	M. V. Azmatunnisa Begum, V.,Rahul, K.,Chandana, A.,Sasikala, C.,Ramana, C. V.		10.1099/ijsem.0.001428	Description of Alteribacillus alkaliphilus sp. nov., reassignment of Bacillus iranensis (Bagheri et al. 2012) as Alteribacillus iranensis comb. nov. and emended description of the genus Alteribacillus	IJSEM 66: 4772-4778 2016	27530438
30213	Barberan A, Caceres Velazquez H, Jones S, Fierer N.		10.1128/mSphere.00237-17	Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information		28776041	26557
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg		10.1093/nar/gkaa1025	BRENDA, the ELIXIR core data resource in 2021: new developments and updates	Nucleic Acids Res. 49: D498-D508 2020	33211880
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481	Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch		10.21203/rs.3.rs-2527258/v1	Predictions based on the model GenomeNet Sporulation v. 1
70967	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID406675.1	StrainInfo: A central database for resolving microbial strain identifiers

General

NCBI tax id

strain history

Name and taxonomic classification

LPSN

synonyms

Morphology

cell morphology

pigmentation

Culture and growth conditions

culture medium

culture temp

culture pH

Physiology and metabolism

oxygen tolerance

spore formation

halophily

metabolite utilization

enzymes

Isolation, sampling and environmental information

isolation

isolation source categories

taxonmaps

Safety information

risk assessment

Sequence information

16S sequences

Genome sequences

GC content

Genome-based predictions

predictions

External links

straininfo link

literature

Reference