Strain identifier

BacDive ID: 131344

Type strain: No

Species: Anaerosalibacter massiliensis

Strain Designation: mAs-G9K2R3-TGB-98

Strain history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; mAs-G9K2R3-TGB-98

NCBI tax ID(s): 1646383 (species)

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General

@ref: 22571

BacDive-ID: 131344

DSM-Number: 29482

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Anaerosalibacter massiliensis mAs-G9K2R3-TGB-98 is an anaerobe, mesophilic bacterium that was isolated from caecal content; wildtype C57BL/6 mouse.

NCBI tax id

  • NCBI tax id: 1646383
  • Matching level: species

strain history

  • @ref: 22571
  • history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; mAs-G9K2R3-TGB-98

doi: 10.13145/bacdive131344.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Tissierellales
  • family: Tissierellaceae
  • genus: Anaerosalibacter
  • species: Anaerosalibacter massiliensis
  • full scientific name: Anaerosalibacter massiliensis Dione et al. 2016

@ref: 22571

domain: Bacteria

phylum: Firmicutes

class: Tissierellia

order: Tissierellales

family: Tissierellaceae

genus: Anaerosalibacter

species: Anaerosalibacter massiliensis

full scientific name: Anaerosalibacter massiliensis Dione et al. 2016

strain designation: mAs-G9K2R3-TGB-98

type strain: no

Morphology

colony morphology

  • @ref: 22571
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

  • @ref: 22571
  • name: PYG MEDIUM (MODIFIED) (DSMZ Medium 104)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104
  • composition: Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

  • @ref: 22571
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 22571
  • oxygen tolerance: anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate+
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
22571----+-+---------+--+/----------

Isolation, sampling and environmental information

isolation

  • @ref: 22571
  • sample type: caecal content; wildtype C57BL/6 mouse
  • geographic location: Freising
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Muridae (Mouse/Rat)
#Host Body-Site#Gastrointestinal tract#Large intestine
#Host Body Product#Gastrointestinal tract#Caecal content

taxonmaps

  • @ref: 69479
  • File name: preview.99_1249.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_207;96_724;97_847;98_987;99_1249&stattab=map
  • Last taxonomy: Anaerosalibacter massiliensis
  • 16S sequence: KR364733
  • Sequence Identity:
  • Total samples: 1600
  • soil counts: 253
  • aquatic counts: 118
  • animal counts: 1206
  • plant counts: 23

Sequence information

16S sequences

  • @ref: 22571
  • description: Anaerosalibacter sp. mAs-G9K2R3-TGB-98 16S ribosomal RNA gene, partial sequence
  • accession: KR364733
  • length: 1419
  • database: ena
  • NCBI tax ID: 1796609

Genome sequences

  • @ref: 66792
  • description: Anaerosalibacter massiliensis DSM 29482
  • accession: GCA_024623155
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 1347392

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno68.91no
gram-positiveyes72.541no
anaerobicyes98.406yes
aerobicno98.077yes
halophileno89.844no
spore-formingyes88.803no
glucose-fermentyes63.083no
thermophileno80.986no
glucose-utilyes89.965no
motileyes84.117no

External links

@ref: 22571

culture collection no.: DSM 29482

straininfo link

  • @ref: 89785
  • straininfo: 400595

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22571Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29482Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29482)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
89785Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID400595.1