Archive BacDiveID:10.13145/bacdive131295.20240916.9.1

Strain identifier

BacDive ID: 131295

Type strain:

Species: Rhodoluna lacicola

Strain Designation: MWH-Ta8

Strain history: <- M. W. Hahn, Austrian Academy of Sci., Inst. Limnology; MWH-Ta8

NCBI tax ID(s): 529884 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 22522

BacDive-ID: 131295

DSM-Number: 23834

keywords: genome sequence, 16S sequence, Bacteria

description: Rhodoluna lacicola MWH-Ta8 is a bacterium that was isolated from planktonic freshwater from a hypertrophic bay of a lake.

NCBI tax id

NCBI tax id: 529884
Matching level: species

strain history

@ref: 22522
history: <- M. W. Hahn, Austrian Academy of Sci., Inst. Limnology; MWH-Ta8

doi: 10.13145/bacdive131295.20240916.9.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/actinomycetota
domain: Bacteria
phylum: Actinomycetota
class: Actinomycetes
order: Micrococcales
family: Microbacteriaceae
genus: Rhodoluna
species: Rhodoluna lacicola
full scientific name: Rhodoluna lacicola Hahn et al. 2014
synonyms
- @ref: 20215
- synonym: Rhodoluna lacicola

@ref: 22522

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Microbacteriaceae

genus: Rhodoluna

species: Rhodoluna lacicola

full scientific name: Rhodoluna lacicola Hahn et al. 2014

strain designation: MWH-Ta8

type strain: yes

Morphology

cell morphology

@ref: 69480
motility: no
confidence: 94

colony morphology

@ref: 22522
incubation period: 8-14 days

Culture and growth conditions

culture medium

@ref: 22522
name: NSY-MEDIUM FOR POLYNUCLEOBACTER (DSMZ Medium 1167)
growth: yes
link: https://mediadive.dsmz.de/medium/1167
composition: Name: NSY-MEDIUM FOR POLYNUCLEOBACTER (DSMZ Medium 1167) Composition: Soy peptone 1.0 g/l Nutrient broth 1.0 g/l Yeast extract 1.0 g/l MgSO4 x 7 H2O 0.075 g/l Ca(NO3)2 x 4 H2O 0.043 g/l NaHCO3 0.016 g/l KCl 0.005 g/l K2HPO4 x 3 H2O 0.0037 g/l Na2-EDTA 5e-05 g/l H3BO3 3e-05 g/l CoCl2 x 6 H2O 2e-05 g/l FeSO4 x 7 H2O 2e-05 g/l ZnSO4 x 7 H2O 1e-05 g/l MnCl2 x 4 H2O 3e-06 g/l Na2MoO4 x 2 H2O 3e-06 g/l NiCl2 x 6 H2O 2e-06 g/l CuCl2 x 2 H2O 1e-06 g/l Tap water Distilled water

culture temp

@ref: 22522
growth: positive
type: growth
temperature: 20

Physiology and metabolism

spore formation

@ref: 69481
spore formation: no
confidence: 100

Isolation, sampling and environmental information

isolation

@ref: 22522
sample type: planktonic freshwater from a hypertrophic bay of a lake
geographic location: Lake Taihu, Meiliang Bay (31° 23' 55'' N 120° 13' 50'' E)
country: China
origin.country: CHN
continent: Asia
latitude: 31.3986
longitude: 120.231

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Lake (large)
#Host	#Protozoa

taxonmaps

@ref: 69479
File name: preview.99_7791.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_1704;97_2040;98_5695;99_7791&stattab=map
Last taxonomy: Rhodoluna lacicola subclade
16S sequence: AM182889
Sequence Identity:
Total samples: 5365
soil counts: 216
aquatic counts: 4244
animal counts: 852
plant counts: 53

Safety information

risk assessment

@ref: 22522
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 22522
description: Rhodoluna lacicola partial 16S rRNA gene, strain MWH-Ta8
accession: AM182889
length: 1423
database: nuccore
NCBI tax ID: 529884

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Rhodoluna lacicola MWH-Ta8	GCA_000699505	complete	ncbi	529884
66792	Rhodoluna lacicola MWH-Ta8	2504643007	complete	img	529884

GC content

@ref: 22522
GC-content: 51.5
method: sequence analysis

Genome-based predictions

predictions

@ref	trait	model	description	prediction	confidence	training_data
69481	spore-forming	spore-forming	Ability to form endo- or exospores	no	100	no
69480	gram-positive	gram-positive	Positive reaction to Gram-staining	yes	84.377	no
69480	anaerobic	anaerobic	Ability to grow under anoxygenic conditions (including facultative anaerobes)	no	92.896	no
69480	aerobic	aerobic	Ability to grow under oxygenic conditions (including facultative aerobes)	yes	75.004	no
69480	spore-forming	spore-forming	Ability to form endo- or exospores	no	89.596	no
69480	thermophilic	thermophile	Ability to grow at temperatures above or equal to 45°C	no	88.894	yes
69480	flagellated	motile2+	Ability to perform flagellated movement	no	94	no

External links

@ref: 22522

culture collection no.: DSM 23834, LMG 26932

straininfo link

@ref: 89737
straininfo: 379774

literature

topic: Phylogeny
Pubmed-ID: 24984700
title: Rhodoluna lacicola gen. nov., sp. nov., a planktonic freshwater bacterium with stream-lined genome.
authors: Hahn MW, Schmidt J, Taipale SJ, Doolittle WF, Koll U
journal: Int J Syst Evol Microbiol
DOI: 10.1099/ijs.0.065292-0
year: 2014
mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fresh Water/*microbiology, Genome, Bacterial, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/chemistry
topic2: Genetics

Reference

@id	authors	title	doi/url	catalogue	journal	pubmed
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ	10.1099/ijsem.0.004332
22522	Curators of the DSMZ		https://www.dsmz.de/collection/catalogue/details/culture/DSM-23834	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23834)
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)	https://diaspora-project.de/progress.html#genomes
66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg	BRENDA, the ELIXIR core data resource in 2021: new developments and updates	10.1093/nar/gkaa1025		Nucleic Acids Res. 49: D498-D508 2020	33211880
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.	MicrobeAtlas 1.0 beta	https://microbeatlas.org/
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)	https://diaspora-project.de/progress.html#genomes
69481	Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch	Predictions based on the model GenomeNet Sporulation v. 1	10.21203/rs.3.rs-2527258/v1
89737	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.	StrainInfo: A central database for resolving microbial strain identifiers	10.60712/SI-ID379774.1

General

NCBI tax id

strain history

Name and taxonomic classification

LPSN

synonyms

Morphology

cell morphology

colony morphology

Culture and growth conditions

culture medium

culture temp

Physiology and metabolism

spore formation

Isolation, sampling and environmental information

isolation

isolation source categories

taxonmaps

Safety information

risk assessment

Sequence information

16S sequences

Genome sequences

GC content

Genome-based predictions

predictions

External links

straininfo link

literature

Reference