Strain identifier
BacDive ID: 131295
Type strain: 
Species: Rhodoluna lacicola
Strain Designation: MWH-Ta8
Strain history: <- M. W. Hahn, Austrian Academy of Sci., Inst. Limnology; MWH-Ta8
NCBI tax ID(s): 529884 (species)
version 8.1 (current version)
General
@ref: 22522
BacDive-ID: 131295
DSM-Number: 23834
keywords: genome sequence, 16S sequence, Bacteria, psychrophilic
description: Rhodoluna lacicola MWH-Ta8 is a psychrophilic bacterium that was isolated from planktonic freshwater from a hypertrophic bay of a lake.
NCBI tax id
- NCBI tax id: 529884
- Matching level: species
strain history
- @ref: 22522
- history: <- M. W. Hahn, Austrian Academy of Sci., Inst. Limnology; MWH-Ta8
doi: 10.13145/bacdive131295.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micrococcales
- family: Microbacteriaceae
- genus: Rhodoluna
- species: Rhodoluna lacicola
- full scientific name: Rhodoluna lacicola Hahn et al. 2014
synonyms
- @ref: 20215
- synonym: Rhodoluna lacicola
@ref: 22522
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Microbacteriaceae
genus: Rhodoluna
species: Rhodoluna lacicola
full scientific name: Rhodoluna lacicola Hahn et al. 2014
strain designation: MWH-Ta8
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | no | 91.415 | |
| 69480 | 100 | positive |
colony morphology
- @ref: 22522
- incubation period: 8-14 days
Culture and growth conditions
culture medium
- @ref: 22522
- name: NSY-MEDIUM FOR POLYNUCLEOBACTER (DSMZ Medium 1167)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1167
- composition: Name: NSY-MEDIUM FOR POLYNUCLEOBACTER (DSMZ Medium 1167) Composition: Soy peptone 1.0 g/l Nutrient broth 1.0 g/l Yeast extract 1.0 g/l MgSO4 x 7 H2O 0.075 g/l Ca(NO3)2 x 4 H2O 0.043 g/l NaHCO3 0.016 g/l KCl 0.005 g/l K2HPO4 x 3 H2O 0.0037 g/l Na2-EDTA 5e-05 g/l H3BO3 3e-05 g/l CoCl2 x 6 H2O 2e-05 g/l FeSO4 x 7 H2O 2e-05 g/l ZnSO4 x 7 H2O 1e-05 g/l MnCl2 x 4 H2O 3e-06 g/l Na2MoO4 x 2 H2O 3e-06 g/l NiCl2 x 6 H2O 2e-06 g/l CuCl2 x 2 H2O 1e-06 g/l Tap water Distilled water
culture temp
- @ref: 22522
- growth: positive
- type: growth
- temperature: 20
- range: psychrophilic
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.999 |
Isolation, sampling and environmental information
isolation
- @ref: 22522
- sample type: planktonic freshwater from a hypertrophic bay of a lake
- geographic location: Lake Taihu, Meiliang Bay (31° 23' 55'' N 120° 13' 50'' E)
- country: China
- origin.country: CHN
- continent: Asia
- latitude: 31.3986
- longitude: 120.231
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Freshwater |
| #Environmental | #Aquatic | #Lake (large) |
| #Host | #Protozoa |
taxonmaps
- @ref: 69479
- File name: preview.99_7791.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_1704;97_2040;98_5695;99_7791&stattab=map
- Last taxonomy: Rhodoluna lacicola subclade
- 16S sequence: AM182889
- Sequence Identity:
- Total samples: 5365
- soil counts: 216
- aquatic counts: 4244
- animal counts: 852
- plant counts: 53
Safety information
risk assessment
- @ref: 22522
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 22522
- description: Rhodoluna lacicola partial 16S rRNA gene, strain MWH-Ta8
- accession: AM182889
- length: 1423
- database: ena
- NCBI tax ID: 529884
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Rhodoluna lacicola MWH-Ta8 | GCA_000699505 | complete | ncbi | 529884 |
| 66792 | Rhodoluna lacicola MWH-Ta8 | 2504643007 | complete | img | 529884 |
GC content
- @ref: 22522
- GC-content: 51.5
- method: sequence analysis
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | no | 95.141 | no |
| flagellated | no | 97.939 | no |
| gram-positive | yes | 86.134 | no |
| anaerobic | no | 98.538 | no |
| halophile | no | 89.933 | no |
| spore-forming | no | 96.606 | no |
| glucose-util | yes | 87.359 | no |
| aerobic | yes | 86.331 | no |
| thermophile | no | 86.325 | no |
| glucose-ferment | no | 81.762 | no |
External links
@ref: 22522
culture collection no.: DSM 23834, LMG 26932
straininfo link
- @ref: 89737
- straininfo: 379774
literature
- topic: Phylogeny
- Pubmed-ID: 24984700
- title: Rhodoluna lacicola gen. nov., sp. nov., a planktonic freshwater bacterium with stream-lined genome.
- authors: Hahn MW, Schmidt J, Taipale SJ, Doolittle WF, Koll U
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.065292-0
- year: 2014
- mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fresh Water/*microbiology, Genome, Bacterial, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/chemistry
- topic2: Genetics
Reference
| @id | authors | title | doi/url | catalogue | journal | pubmed |
|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
| 22522 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23834 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23834) | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |||
| 89737 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID379774.1 |