Strain identifier

BacDive ID: 13116

Type strain: Yes

Species: Pseudomonas orientalis

Strain history: CIP <- 1998, F. Dabboussi, Fac. Med., Lille, France: strain CFML 96-170

NCBI tax ID(s): 76758 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 7017

BacDive-ID: 13116

DSM-Number: 17489

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Pseudomonas orientalis DSM 17489 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from spring water.

NCBI tax id

  • NCBI tax id: 76758
  • Matching level: species

strain history

@refhistory
7017<- D. Izard <- M. Hamze; CFML 96-170
121439CIP <- 1998, F. Dabboussi, Fac. Med., Lille, France: strain CFML 96-170

doi: 10.13145/bacdive13116.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas orientalis
  • full scientific name: Pseudomonas orientalis Dabboussi et al. 2002

@ref: 7017

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas orientalis

full scientific name: Pseudomonas orientalis Dabboussi et al. 2002

type strain: yes

Morphology

cell morphology

  • @ref: 121439
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 121439
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7017TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
7017NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
39309MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
121439CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
7017positivegrowth30mesophilic
39309positivegrowth30mesophilic
121439positivegrowth5-41

Physiology and metabolism

oxygen tolerance

  • @ref: 121439
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
121439NaClpositivegrowth0-6 %
121439NaClnogrowth8 %
121439NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
121439citrate+carbon source16947
121439esculin-hydrolysis4853
121439nitrate-reduction17632
121439nitrite-reduction16301
121439nitrate-respiration17632

antibiotic resistance

  • @ref: 121439
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 121439
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
121439oxidase+
121439beta-galactosidase-3.2.1.23
121439alcohol dehydrogenase+1.1.1.1
121439gelatinase+
121439amylase+
121439DNase-
121439caseinase+3.4.21.50
121439catalase+1.11.1.6
121439tween esterase+
121439lecithinase+
121439lipase-
121439lysine decarboxylase-4.1.1.18
121439ornithine decarboxylase-4.1.1.17
121439protease+
121439tryptophan deaminase-
121439urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121439--++-+--+-+---------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121439-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121439+++++--++---------++++-+--+++--+++--++-----++----+++-++++-+++-+++-----++++-++++++++++-+-+++++++++++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
7017spring waterLebanonLBNAsia
121439Environment, Spring waterLebanonLBNAsia1995

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Spring

taxonmaps

  • @ref: 69479
  • File name: preview.99_168.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_98;99_168&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: AF064457
  • Sequence Identity:
  • Total samples: 5463
  • soil counts: 555
  • aquatic counts: 976
  • animal counts: 2173
  • plant counts: 1759

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
70171Risk group (German classification)
1214391Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7017
  • description: Pseudomonas orientalis 16S ribosomal RNA gene, complete sequence
  • accession: AF064457
  • length: 1516
  • database: ena
  • NCBI tax ID: 76758

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas orientalis DSM 17489GCA_001439815contigncbi76758
66792Pseudomonas orientalis strain DSM 1748976758.3wgspatric76758
66792Pseudomonas orientalis DSM 174892663762900draftimg76758

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes90.18no
flagellatedyes82.206no
gram-positiveno98.425no
anaerobicno98.096no
halophileno91.86no
spore-formingno95.555no
glucose-utilyes94.69no
aerobicyes93.385no
thermophileno99.779no
glucose-fermentno91.394no

External links

@ref: 7017

culture collection no.: DSM 17489, CIP 105540, CFML 96-170

straininfo link

  • @ref: 82324
  • straininfo: 69810

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10422691Taxonomic study of bacteria isolated from Lebanese spring waters: proposal for Pseudomonas cedrella sp. nov. and P. orientalis sp. nov.Dabboussi F, Hamze M, Elomari M, Verhille S, Baida N, Izard D, Leclerc HRes Microbiol10.1016/s0923-2508(99)80056-41999Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics/isolation & purification, DNA, Ribosomal/analysis, Fresh Water/microbiology, Genes, rRNA, Lebanon, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Pseudomonas/*classification/genetics/*isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Water MicrobiologyEnzymology
Phylogeny31922466Pseudomonas carnis sp. nov., isolated from meat.Lick S, Krockel L, Wibberg D, Winkler A, Blom J, Bantleon A, Goesmann A, Kalinowski JInt J Syst Evol Microbiol10.1099/ijsem.0.0039282020Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Food Microbiology, Genes, Bacterial, Germany, Meat/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Poultry, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Swine, Ubiquinone/chemistryBiotechnology

Reference

@idauthorscataloguedoi/urltitle
7017Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17489)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17489
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39309Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17571
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82324Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID69810.1StrainInfo: A central database for resolving microbial strain identifiers
121439Curators of the CIPCollection of Institut Pasteur (CIP 105540)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105540