Strain identifier

BacDive ID: 13112

Type strain: Yes

Species: Pseudomonas libanensis

Strain history: CIP <- 1998, F. Dabboussi, Henri Warembourg Fac. Med., Lille, France: strain CFML 96-195

NCBI tax ID(s): 75588 (species)

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General

@ref: 6790

BacDive-ID: 13112

DSM-Number: 17149

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Pseudomonas libanensis CCUG 43190 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from spring water.

NCBI tax id

  • NCBI tax id: 75588
  • Matching level: species

strain history

@refhistory
6790<- CCM <- CCUG <- CIP <- F. Daboussi
120591CIP <- 1998, F. Dabboussi, Henri Warembourg Fac. Med., Lille, France: strain CFML 96-195

doi: 10.13145/bacdive13112.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas libanensis
  • full scientific name: Pseudomonas libanensis Dabboussi et al. 1999

@ref: 6790

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas libanensis

full scientific name: Pseudomonas libanensis Dabboussi et al. 1999

type strain: yes

Morphology

cell morphology

  • @ref: 120591
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 120591
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6790CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
32714MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
120591CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
6790positivegrowth28mesophilic
32714positivegrowth30mesophilic
55716positivegrowth30mesophilic
120591positivegrowth5-37
120591nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
55716aerobe
120591obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
120591NaClpositivegrowth0-6 %
120591NaClnogrowth8 %
120591NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction
12059116947citrate+carbon source
1205914853esculin-hydrolysis
12059117632nitrate+reduction
12059116301nitrite-reduction
12059117632nitrate+respiration

antibiotic resistance

  • @ref: 120591
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12059135581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6
120591oxidase+
120591beta-galactosidase-3.2.1.23
120591alcohol dehydrogenase+1.1.1.1
120591gelatinase+/-
120591amylase-
120591DNase-
120591caseinase+3.4.21.50
120591catalase+1.11.1.6
120591tween esterase-
120591lecithinase+
120591lipase-
120591lysine decarboxylase-4.1.1.18
120591ornithine decarboxylase-4.1.1.17
120591tryptophan deaminase-
120591urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120591--++-+--+-----------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
6790+--+--+-+++++-++-++-+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120591+++++--------------+++----+++--+++--++--+--++----+++-++++-+++-+++------+++-+++++++--+-+++++++++++--

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dateisolation date
6790spring waterLebanonLBNAsia
55716Spring waterLebanonLBNAsia1995
120591Environment, Spring waterLebanonLBNAsia1995

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Spring

taxonmaps

  • @ref: 69479
  • File name: preview.99_199.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_98;99_199&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: AF057645
  • Sequence Identity:
  • Total samples: 8172
  • soil counts: 874
  • aquatic counts: 1631
  • animal counts: 3216
  • plant counts: 2451

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
67901Risk group (German classification)
1205911Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6790
  • description: Pseudomonas libaniensis 16S ribosomal RNA gene, complete sequence
  • accession: AF057645
  • length: 1516
  • database: ena
  • NCBI tax ID: 75588

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas libanensis DSM 17149GCA_001439685contigncbi75588
66792Pseudomonas libanensis strain DSM 1714975588.4wgspatric75588
66792Pseudomonas libanensis DSM 171492636416169draftimg75588
66792Pseudomonas libanensis DSM 171492700989493draftimg75588
66792Pseudomonas libanensis DSM 17149GCA_900101035chromosomencbi75588

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes89.571no
flagellatedyes79.392no
gram-positiveno98.251no
anaerobicno98.241yes
aerobicyes95.147yes
halophileno92.618no
spore-formingno94.876no
glucose-fermentno89.44no
thermophileno99.852yes
glucose-utilyes95.013yes

External links

@ref: 6790

culture collection no.: CCUG 43190, DSM 17149, CCM 4841, CFML 96-195, CIP 105460

straininfo link

  • @ref: 82320
  • straininfo: 69804

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10425766Pseudomonas libanensis sp. nov., a new species isolated from Lebanese spring waters.Dabboussi F, Hamze M, Elomari M, Verhille S, Baida N, Izard D, Leclerc HInt J Syst Bacteriol10.1099/00207713-49-3-10911999Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fresh Water/*microbiology, Lebanon, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Pseudomonas/*classification/cytology/isolation & purification/physiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Water MicrobiologyGenetics
Phylogeny20232682Isolation, identification and characterization of a new lipolytic pseudomonas sp., strain AHD-1, from Tunisian soil.Fendri I, Chaari A, Dhouib A, Jlassi B, Abousalham A, Carriere F, Sayadi S, Abdelkafi SEnviron Technol10.1080/095933309033699942010Lipid Metabolism/*physiology, Lipolysis/*physiology, Pseudomonas/*classification/isolation & purification/*physiology, *Soil Microbiology, Species Specificity, TunisiaMetabolism
Phylogeny28141500Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp. nov., isolated from bovine raw milk.von Neubeck M, Huptas C, Gluck C, Krewinkel M, Stoeckel M, Stressler T, Fischer L, Hinrichs J, Scherer S, Wenning MInt J Syst Evol Microbiol10.1099/ijsem.0.0018362017Animals, Bacterial Typing Techniques, Base Composition, Cattle, DNA, Bacterial/genetics, Fatty Acids/chemistry, Milk/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pseudomonas/*classification/genetics/isolation & purification, Quinones/chemistry, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome
Phylogeny31922466Pseudomonas carnis sp. nov., isolated from meat.Lick S, Krockel L, Wibberg D, Winkler A, Blom J, Bantleon A, Goesmann A, Kalinowski JInt J Syst Evol Microbiol10.1099/ijsem.0.0039282020Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Food Microbiology, Genes, Bacterial, Germany, Meat/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Poultry, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Swine, Ubiquinone/chemistryBiotechnology
Phylogeny34309505Pseudomonas lactucae sp. nov., a pathogen causing bacterial rot of lettuce in Japan.Sawada H, Fujikawa T, Satou MInt J Syst Evol Microbiol10.1099/ijsem.0.0049172021Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Japan, Lettuce/*microbiology, Nucleic Acid Hybridization, *Phylogeny, Plant Diseases/*microbiology, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitle
6790Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17149)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17149
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32714Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17482
55716Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 43190)https://www.ccug.se/strain?id=43190
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82320Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID69804.1StrainInfo: A central database for resolving microbial strain identifiers
120591Curators of the CIPCollection of Institut Pasteur (CIP 105460)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105460