Strain identifier

BacDive ID: 131087

Type strain: Yes

Species: Enterobacter hormaechei subsp. oharae

Strain Designation: EN-314

Strain history: CIP <- 2004, J. Heesemann, LM Münich Univ., Gauting, Germany: strain EN-314 <- S. Ziesing

NCBI tax ID(s): 301102 (subspecies)

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General

@ref: 22379

BacDive-ID: 131087

DSM-Number: 16687

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, motile

description: Enterobacter hormaechei subsp. oharae EN-314 is an aerobe, mesophilic, motile bacterium that was isolated from mouth swab of 2 year-old infant.

NCBI tax id

  • NCBI tax id: 301102
  • Matching level: subspecies

strain history

@refhistory
22379<- H. Hoffmann, Institute of Microbiology, Pneumological Teaching Hospital of the University of Munich; EN-314 <- S. Ziesing, Medizinische Hochschule Hannover
119970CIP <- 2004, J. Heesemann, LM Münich Univ., Gauting, Germany: strain EN-314 <- S. Ziesing

doi: 10.13145/bacdive131087.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Enterobacter
  • species: Enterobacter hormaechei subsp. oharae
  • full scientific name: Enterobacter hormaechei subsp. oharae Hoffmann et al. 2016

@ref: 22379

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Enterobacter

species: Enterobacter hormaechei subsp. oharae

full scientific name: Enterobacter hormaechei subsp. oharae Hoffmann et al. 2016

strain designation: EN-314

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes96.978
6948099.99negative
119970yesnegativerod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
22379TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
22379LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)yeshttps://mediadive.dsmz.de/medium/381Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water
22379CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
41956MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119970CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
22379positivegrowth37mesophilic
41956positivegrowth30mesophilic
60073positivegrowth30-37mesophilic
119970positivegrowth10-41
119970nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
60073aerobe
119970facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.914

compound production

  • @ref: 22379
  • compound: beta lactamase

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11997029864mannitol+fermentation
11997016947citrate+carbon source
11997017234glucose+fermentation
11997017716lactose-fermentation
11997017632nitrate+reduction
11997016301nitrite+reduction
11997015792malonate+assimilation
119970132112sodium thiosulfate-builds gas from

antibiotic resistance

  • @ref: 119970
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 119970
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11997015688acetoin+
11997017234glucose-

enzymes

@refvalueactivityec
119970oxidase-
119970beta-galactosidase+3.2.1.23
119970alcohol dehydrogenase+1.1.1.1
119970gelatinase+
119970catalase+1.11.1.6
119970lysine decarboxylase-4.1.1.18
119970ornithine decarboxylase+4.1.1.17
119970phenylalanine ammonia-lyase-4.3.1.24
119970tryptophan deaminase-
119970urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119970-+---+----++++------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119970+++++-++++++-++++++++++++------+--+++--+-+-++----+++-++++--+++-------------+--+++-+---+--+++++++---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
22379mouth swab of 2 year-old infantHannoverGermanyDEUEurope
60073Human mouthHannoverGermanyDEUEurope2002
119970Human, MouthHanoverGermanyDEUEurope2002

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Infection#Patient#Swab
#Host Body-Site#Oral cavity and airways#Mouth
#Host#Human#Child

taxonmaps

  • @ref: 69479
  • File name: preview.99_66.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_7;98_17;99_66&stattab=map
  • Last taxonomy: Enterobacterales
  • 16S sequence: AJ853889
  • Sequence Identity:
  • Total samples: 14221
  • soil counts: 575
  • aquatic counts: 888
  • animal counts: 11838
  • plant counts: 920

Safety information

risk assessment

  • @ref: 119970
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 22379
  • description: Enterobacter hormaechei subsp. oharae 16S rRNA gene, type strain EN-314T
  • accession: AJ853889
  • length: 1515
  • database: ena
  • NCBI tax ID: 301102

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterobacter hormaechei subsp. oharae DSM 16687GCA_001729705completencbi301102
66792Enterobacter hormaechei subsp. oharae strain DSM 16687301102.37completepatric301102
66792Enterobacter hormaechei subsp. oharae strain FDAARGOS_1533301102.80completepatric301102
66792Enterobacter hormaechei oharae DSM 166872721755540completeimg301102
66792Enterobacter hormaechei subsp. oharae strain FDAARGOS_1533301102.83completepatric301102
66792Enterobacter hormaechei subsp. oharae strain FDAARGOS_1533301102.81completepatric301102
66792Enterobacter hormaechei subsp. oharae strain FDAARGOS_1533301102.82completepatric301102

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno60.098no
gram-positiveno97.971no
anaerobicno96.077yes
aerobicyes89.078yes
halophileno84.94no
spore-formingno92.526no
thermophileno99.3no
glucose-utilyes94.28no
motileyes88.777no
glucose-fermentyes93.256no

External links

@ref: 22379

culture collection no.: DSM 16687, CCM 8660, CCUG 53905, CIP 108490

straininfo link

  • @ref: 89549
  • straininfo: 134558

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22379Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-16687Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16687)
41956Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6118
60073Curators of the CCUGhttps://www.ccug.se/strain?id=53905Culture Collection University of Gothenburg (CCUG) (CCUG 53905)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
89549Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID134558.1
119970Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108490Collection of Institut Pasteur (CIP 108490)