Strain identifier
BacDive ID: 130947
Type strain: 
Species: Enterococcus hirae
Strain Designation: SB
Strain history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; SB
NCBI tax ID(s): 1354 (species)
version 9.1 (current version)
General
@ref: 22239
BacDive-ID: 130947
DSM-Number: 28619
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, Gram-positive, human pathogen
description: Enterococcus hirae SB is a microaerophile, Gram-positive human pathogen that was isolated from ileal content; wildtype C57BL/6 mouse.
NCBI tax id
- NCBI tax id: 1354
- Matching level: species
strain history
- @ref: 22239
- history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; SB
doi: 10.13145/bacdive130947.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Enterococcaceae
- genus: Enterococcus
- species: Enterococcus hirae
- full scientific name: Enterococcus hirae Farrow and Collins 1985
@ref: 22239
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Enterococcaceae
genus: Enterococcus
species: Enterococcus hirae
full scientific name: Enterococcus hirae Farrow and Collins 1985
strain designation: SB
type strain: no
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 92.952
colony morphology
- @ref: 22239
- incubation period: 1-2 days
Culture and growth conditions
culture medium
- @ref: 22239
- name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)
- growth: yes
- link: https://mediadive.dsmz.de/medium/92
- composition: Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
culture temp
- @ref: 22239
- growth: positive
- type: growth
- temperature: 37
Physiology and metabolism
oxygen tolerance
- @ref: 22239
- oxygen tolerance: microaerophile
spore formation
- @ref: 69481
- spore formation: yes
- confidence: 97
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68381 | 29016 | arginine | + | hydrolysis |
| 68381 | 16988 | D-ribose | + | builds acid from |
| 68381 | 16899 | D-mannitol | - | builds acid from |
| 68381 | 30911 | sorbitol | - | builds acid from |
| 68381 | 17716 | lactose | - | builds acid from |
| 68381 | 27082 | trehalose | + | builds acid from |
| 68381 | 16634 | raffinose | - | builds acid from |
| 68381 | 17992 | sucrose | + | builds acid from |
| 68381 | 30849 | L-arabinose | - | builds acid from |
| 68381 | 18333 | D-arabitol | - | builds acid from |
| 68381 | 40585 | alpha-cyclodextrin | + | builds acid from |
| 68381 | 28087 | glycogen | - | builds acid from |
| 68381 | 27941 | pullulan | - | builds acid from |
| 68381 | 17306 | maltose | + | builds acid from |
| 68381 | 28053 | melibiose | - | builds acid from |
| 68381 | 6731 | melezitose | - | builds acid from |
| 68381 | 320055 | methyl beta-D-glucopyranoside | + | builds acid from |
| 68381 | 16443 | D-tagatose | - | builds acid from |
| 68381 | 16199 | urea | - | hydrolysis |
metabolite production
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- production: yes
metabolite tests
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: +
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68381 | urease | - | 3.5.1.5 |
| 68381 | glycyl tryptophan arylamidase | + | |
| 68381 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
| 68381 | pyrrolidonyl arylamidase | + | 3.4.19.3 |
| 68381 | beta-galactosidase | - | 3.2.1.23 |
| 68381 | Alanyl-Phenylalanyl-Proline arylamidase | - | |
| 68381 | alkaline phosphatase | - | 3.1.3.1 |
| 68381 | alpha-galactosidase | - | 3.2.1.22 |
| 68381 | beta-glucosidase | + | 3.2.1.21 |
| 68381 | arginine dihydrolase | + | 3.5.3.6 |
API rID32STR
| @ref | ADH Arg | beta GLU | beta GAR | beta GUR | alpha GAL | PAL | RIB | MAN | SOR | LAC | TRE | RAF | SAC | LARA | DARL | CDEX | VP | APPA | beta GAL | PyrA | beta NAG | GTA | HIP | GLYG | PUL | MAL | MEL | MLZ | Mbeta DG | TAG | beta MAN | URE |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 22239 | + | + | - | + | - | - | + | - | - | - | + | - | + | - | - | + | + | - | - | + | + | + | +/- | - | - | + | - | - | + | - | - | - |
| 22239 | + | + | - | - | - | - | + | - | - | - | + | - | + | - | - | + | + | - | - | + | + | + | - | - | - | + | - | - | + | - | + | - |
Isolation, sampling and environmental information
isolation
- @ref: 22239
- sample type: ileal content; wildtype C57BL/6 mouse
- geographic location: Freising
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Mammals | #Muridae (Mouse/Rat) |
| #Host Body-Site | #Gastrointestinal tract | #Small intestine |
taxonmaps
- @ref: 69479
- File name: preview.99_23.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_19;97_19;98_22;99_23&stattab=map
- Last taxonomy: Enterococcus
- 16S sequence: KR364767
- Sequence Identity:
- Total samples: 55544
- soil counts: 1007
- aquatic counts: 2532
- animal counts: 51351
- plant counts: 654
Safety information
risk assessment
- @ref: 22239
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 22239
- description: Enterococcus sp. SB 16S ribosomal RNA gene, partial sequence
- accession: KR364767
- length: 1419
- database: nuccore
- NCBI tax ID: 1796632
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Enterococcus hirae strain FDAARGOS_1125 | 1354.361 | complete | patric | 1354 |
| 66792 | Enterococcus hirae DSM 28619 | GCA_024622605 | contig | ncbi | 1354 |
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69481 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 97 | no |
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 92.952 | no |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 88.244 | no |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 76.422 | no |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 95.214 | no |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 97.5 | yes |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | no | 88.167 | no |
External links
@ref: 22239
culture collection no.: DSM 28619
straininfo link
- @ref: 89424
- straininfo: 398670
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 22239 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-28619 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28619) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 68381 | Automatically annotated from API rID32STR | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
| 89424 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID398670.1 |