Strain identifier

BacDive ID: 130947

Type strain: No

Species: Enterococcus hirae

Strain Designation: SB

Strain history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; SB

NCBI tax ID(s): 1354 (species)

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General

@ref: 22239

BacDive-ID: 130947

DSM-Number: 28619

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, spore-forming, mesophilic, Gram-positive, human pathogen

description: Enterococcus hirae SB is a microaerophile, spore-forming, mesophilic human pathogen that was isolated from ileal content; wildtype C57BL/6 mouse.

NCBI tax id

  • NCBI tax id: 1354
  • Matching level: species

strain history

  • @ref: 22239
  • history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; SB

doi: 10.13145/bacdive130947.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus hirae
  • full scientific name: Enterococcus hirae Farrow and Collins 1985

@ref: 22239

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus hirae

full scientific name: Enterococcus hirae Farrow and Collins 1985

strain designation: SB

type strain: no

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

colony morphology

  • @ref: 22239
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

  • @ref: 22239
  • name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/92
  • composition: Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

  • @ref: 22239
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 22239
  • oxygen tolerance: microaerophile

spore formation

@refspore formationconfidence
69481yes97
69480no99.973

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838129016arginine+hydrolysis
6838116988D-ribose+builds acid from
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose-builds acid from
6838127082trehalose+builds acid from
6838116634raffinose-builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin+builds acid from
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside+builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
22239++-+--+---+-+--++--++++/---+--+---
22239++----+---+-+--++--+++---+--+-+-

Isolation, sampling and environmental information

isolation

  • @ref: 22239
  • sample type: ileal content; wildtype C57BL/6 mouse
  • geographic location: Freising
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Muridae (Mouse/Rat)
#Host Body-Site#Gastrointestinal tract#Small intestine

taxonmaps

  • @ref: 69479
  • File name: preview.99_23.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_19;97_19;98_22;99_23&stattab=map
  • Last taxonomy: Enterococcus
  • 16S sequence: KR364767
  • Sequence Identity:
  • Total samples: 55544
  • soil counts: 1007
  • aquatic counts: 2532
  • animal counts: 51351
  • plant counts: 654

Safety information

risk assessment

  • @ref: 22239
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22239
  • description: Enterococcus sp. SB 16S ribosomal RNA gene, partial sequence
  • accession: KR364767
  • length: 1419
  • database: ena
  • NCBI tax ID: 1796632

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus hirae strain FDAARGOS_11251354.361completepatric1354
66792Enterococcus hirae DSM 28619GCA_024622605contigncbi1354

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes97no
motileno95.522no
gram-positiveyes94.283no
anaerobicno95.416yes
halophileyes89.337no
spore-formingno90.873no
thermophileno99.759yes
glucose-utilyes87.114no
flagellatedno97.545no
aerobicno96.893yes
glucose-fermentyes90.615no

External links

@ref: 22239

culture collection no.: DSM 28619

straininfo link

  • @ref: 89424
  • straininfo: 398670

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22239Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28619Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28619)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68381Automatically annotated from API rID32STR
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
89424Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID398670.1