Strain identifier

BacDive ID: 130924

Type strain: No

Species: Clostridium perfringens

Strain Designation: MJJ0609-4-1

Strain history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; MJJ0609-4-1

NCBI tax ID(s): 1502 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 22216

BacDive-ID: 130924

DSM-Number: 29991

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, rod-shaped, human pathogen

description: Clostridium perfringens MJJ0609-4-1 is an anaerobe, spore-forming, mesophilic human pathogen that was isolated from wild mouse.

NCBI tax id

  • NCBI tax id: 1502
  • Matching level: species

strain history

  • @ref: 22216
  • history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; MJJ0609-4-1

doi: 10.13145/bacdive130924.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium perfringens
  • full scientific name: Clostridium perfringens (Veillon and Zuber 1898) Hauduroy et al. 1937 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacillus perfringens

@ref: 22216

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium perfringens

full scientific name: Clostridium perfringens (Veillon and Zuber 1898) Hauduroy et al. 1937

strain designation: MJJ0609-4-1

type strain: no

Morphology

cell morphology

@refcell shapegram stainconfidence
68367rod-shaped
68367positive
69480positive100

colony morphology

  • @ref: 22216
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
22216FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203)yeshttps://mediadive.dsmz.de/medium/1203Name: FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203) Composition: Horse blood 100.0 g/l Fastidious Anaerobe Agar 45.7 g/l Distilled water
22216CHOPPED MEAT MEDIUM (DSMZ Medium 78)yeshttps://mediadive.dsmz.de/medium/78Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water
22216WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)yeshttps://mediadive.dsmz.de/medium/339Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

  • @ref: 22216
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
22216anaerobe
69480anaerobe98.651

spore formation

@refspore formationconfidence
68367yes
69481yes100
69480yes99.999

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836727082trehalose+builds acid from
6836716024D-mannose+builds acid from
6836717754glycerol+builds acid from
683674853esculin+hydrolysis
683675291gelatin+hydrolysis
6836717306maltose+builds acid from
6836717992sucrose+builds acid from
6836717716lactose+builds acid from
6836717634D-glucose+builds acid from
6836716199urea-hydrolysis
6836727897tryptophan-energy source

metabolite production

  • @ref: 68367
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68367
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68367catalase+1.11.1.6
68367beta-glucosidase+3.2.1.21
68367gelatinase+
68367urease-3.5.1.5

API 20A

@refINDUREGLUMANLACSACMALSALXYLARAGELESCGLYCELMNEMLZRAFSORRHATRECATSPORGRAMCOCC
22216--+++++++++++++++++++++-
22216--+-+++---+++-+----++++-

Isolation, sampling and environmental information

isolation

  • @ref: 22216
  • sample type: wild mouse
  • geographic location: Freising
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

  • Cat1: #Host
  • Cat2: #Mammals
  • Cat3: #Muridae (Mouse/Rat)

taxonmaps

  • @ref: 69479
  • File name: preview.99_335.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_235;97_257;98_286;99_335&stattab=map
  • Last taxonomy: Clostridium perfringens subclade
  • 16S sequence: KR364753
  • Sequence Identity:
  • Total samples: 88326
  • soil counts: 3063
  • aquatic counts: 8762
  • animal counts: 75654
  • plant counts: 847

Safety information

risk assessment

  • @ref: 22216
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22216
  • description: Clostridium perfringens strain WCA-an-CC-1-3a 16S ribosomal RNA gene, partial sequence
  • accession: KR364753
  • length: 1462
  • database: ena
  • NCBI tax ID: 1502

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Clostridium perfringens strain FDAARGOS_9311502.1786completepatric1502
66792Clostridium perfringens DSM 29991GCA_024622845contigncbi1502

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno79.044no
gram-positiveyes93.029yes
anaerobicyes97.963yes
aerobicno96.354no
halophileno70.547no
spore-formingyes90.961yes
thermophileno99.006yes
glucose-utilyes90.587no
flagellatedno91.422no
glucose-fermentyes77.623no

External links

@ref: 22216

culture collection no.: DSM 29991

straininfo link

  • @ref: 89404
  • straininfo: 399924

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22216Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29991Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29991)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68367Automatically annotated from API 20A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
89404Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID399924.1