Strain identifier
BacDive ID: 130924
Type strain: 
Species: Clostridium perfringens
Strain Designation: MJJ0609-4-1
Strain history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; MJJ0609-4-1
NCBI tax ID(s): 1502 (species)
version 9.1 (current version)
General
@ref: 22216
BacDive-ID: 130924
DSM-Number: 29991
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, rod-shaped, human pathogen
description: Clostridium perfringens MJJ0609-4-1 is an anaerobe, spore-forming, rod-shaped human pathogen that was isolated from wild mouse.
NCBI tax id
- NCBI tax id: 1502
- Matching level: species
strain history
- @ref: 22216
- history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; MJJ0609-4-1
doi: 10.13145/bacdive130924.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Clostridium
- species: Clostridium perfringens
- full scientific name: Clostridium perfringens (Veillon and Zuber 1898) Hauduroy et al. 1937 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Bacillus perfringens
@ref: 22216
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Clostridiaceae
genus: Clostridium
species: Clostridium perfringens
full scientific name: Clostridium perfringens (Veillon and Zuber 1898) Hauduroy et al. 1937
strain designation: MJJ0609-4-1
type strain: no
Morphology
cell morphology
| @ref | cell shape | gram stain |
|---|---|---|
| 68367 | rod-shaped | |
| 68367 | positive |
colony morphology
- @ref: 22216
- incubation period: 2-3 days
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 22216 | FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203) | yes | https://mediadive.dsmz.de/medium/1203 | Name: FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203) Composition: Horse blood 100.0 g/l Fastidious Anaerobe Agar 45.7 g/l Distilled water |
| 22216 | CHOPPED MEAT MEDIUM (DSMZ Medium 78) | yes | https://mediadive.dsmz.de/medium/78 | Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water |
| 22216 | WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) | yes | https://mediadive.dsmz.de/medium/339 | Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water |
culture temp
- @ref: 22216
- growth: positive
- type: growth
- temperature: 37
Physiology and metabolism
oxygen tolerance
- @ref: 22216
- oxygen tolerance: anaerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 68367 | yes | |
| 69481 | yes | 100 |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68367 | 27082 | trehalose | + | builds acid from |
| 68367 | 16024 | D-mannose | + | builds acid from |
| 68367 | 17754 | glycerol | + | builds acid from |
| 68367 | 4853 | esculin | + | hydrolysis |
| 68367 | 5291 | gelatin | + | hydrolysis |
| 68367 | 17306 | maltose | + | builds acid from |
| 68367 | 17992 | sucrose | + | builds acid from |
| 68367 | 17716 | lactose | + | builds acid from |
| 68367 | 17634 | D-glucose | + | builds acid from |
| 68367 | 16199 | urea | - | hydrolysis |
| 68367 | 27897 | tryptophan | - | energy source |
metabolite production
- @ref: 68367
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68367
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68367 | catalase | + | 1.11.1.6 |
| 68367 | beta-glucosidase | + | 3.2.1.21 |
| 68367 | gelatinase | + | |
| 68367 | urease | - | 3.5.1.5 |
API 20A
| @ref | IND | URE | GLU | MAN | LAC | SAC | MAL | SAL | XYL | ARA | GEL | ESC | GLY | CEL | MNE | MLZ | RAF | SOR | RHA | TRE | CAT | SPOR | GRAM | COCC |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 22216 | - | - | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | - |
| 22216 | - | - | + | - | + | + | + | - | - | - | + | + | + | - | + | - | - | - | - | + | + | + | + | - |
Isolation, sampling and environmental information
isolation
- @ref: 22216
- sample type: wild mouse
- geographic location: Freising
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
- Cat1: #Host
- Cat2: #Mammals
- Cat3: #Muridae (Mouse/Rat)
taxonmaps
- @ref: 69479
- File name: preview.99_335.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_235;97_257;98_286;99_335&stattab=map
- Last taxonomy: Clostridium perfringens subclade
- 16S sequence: KR364753
- Sequence Identity:
- Total samples: 88326
- soil counts: 3063
- aquatic counts: 8762
- animal counts: 75654
- plant counts: 847
Safety information
risk assessment
- @ref: 22216
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 22216
- description: Clostridium perfringens strain WCA-an-CC-1-3a 16S ribosomal RNA gene, partial sequence
- accession: KR364753
- length: 1462
- database: nuccore
- NCBI tax ID: 1502
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Clostridium perfringens strain FDAARGOS_931 | 1502.1786 | complete | patric | 1502 |
| 66792 | Clostridium perfringens DSM 29991 | GCA_024622845 | contig | ncbi | 1502 |
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69481 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 100 | no |
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 73.135 | no |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | yes | 87.843 | no |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 76.252 | yes |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 94.822 | no |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 97.223 | yes |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | no | 66.205 | no |
External links
@ref: 22216
culture collection no.: DSM 29991
straininfo link
- @ref: 89404
- straininfo: 399924
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 22216 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-29991 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29991) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 68367 | Automatically annotated from API 20A | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
| 89404 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID399924.1 |