Strain identifier

BacDive ID: 13085

Type strain: Yes

Species: Pseudomonas congelans

Strain Designation: P538/23

Strain history: CIP <- 2003, U. Behrendt, Paulinenaue, Germany: strain P538/23

NCBI tax ID(s): 200452 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 5612

BacDive-ID: 13085

DSM-Number: 14939

keywords: genome sequence, 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative, motile, rod-shaped

description: Pseudomonas congelans P538/23 is an aerobe, psychrophilic, Gram-negative bacterium that was isolated from phyllosphere of grasses.

NCBI tax id

  • NCBI tax id: 200452
  • Matching level: species

strain history

@refhistory
5612<- U. Behrendt; P538/23
397522003, U. Behrendt, Paulinenaue, Germany: strain P538/23
118729CIP <- 2003, U. Behrendt, Paulinenaue, Germany: strain P538/23

doi: 10.13145/bacdive13085.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas congelans
  • full scientific name: Pseudomonas congelans Behrendt et al. 2003

@ref: 5612

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas congelans

full scientific name: Pseudomonas congelans Behrendt et al. 2003

strain designation: P538/23

type strain: yes

Morphology

cell morphology

  • @ref: 118729
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 59223
  • incubation period: 1 day

pigmentation

  • @ref: 118729
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5612REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a)yeshttps://mediadive.dsmz.de/medium/220aName: REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a) Composition: Casein peptone 17.0 g/l Agar 15.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water
5612REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)yeshttps://mediadive.dsmz.de/medium/1aName: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
39752MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
118729CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5612positivegrowth22psychrophilic
39752positivegrowth30mesophilic
59223positivegrowth30mesophilic
118729positivegrowth5-30
118729nogrowth37mesophilic
118729nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
59223aerobe
118729obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
118729NaClpositivegrowth0-6 %
118729NaClnogrowth8 %
118729NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
11872916947citrate+carbon source
1187294853esculin+hydrolysis
11872917632nitrate-reduction
11872916301nitrite-reduction
11872917632nitrate-respiration

antibiotic resistance

  • @ref: 118729
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11872935581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
118729oxidase-
118729beta-galactosidase-3.2.1.23
118729alcohol dehydrogenase-1.1.1.1
118729gelatinase-
118729amylase+
118729DNase-
118729caseinase+3.4.21.50
118729catalase+1.11.1.6
118729tween esterase-
118729lecithinase-
118729lipase-
118729lysine decarboxylase-4.1.1.18
118729ornithine decarboxylase-4.1.1.17
118729protease-
118729tryptophan deaminase-
118729urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118729-+++-++-+-++----+---

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
5612-----++-++++--++-++-+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118729+++-+--+----------++++----+----+++--+---+--++--+++++-+++----+-+++-----++-+-+++-++++---+-+++++++++-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5612phyllosphere of grassesBrandenburg, PaulinenaueGermanyDEUEurope
59223Phyllosphere of grassesPaulinenaue BrandenburgGermanyDEUEurope
118729Phyllosphere of grassesGermanyDEUEurope1994

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Leaf (Phyllosphere)

taxonmaps

  • @ref: 69479
  • File name: preview.99_108.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_98;99_108&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: AJ492828
  • Sequence Identity:
  • Total samples: 1469
  • soil counts: 744
  • aquatic counts: 346
  • animal counts: 234
  • plant counts: 145

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
56121Risk group (German classification)
1187291Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5612
  • description: Pseudomonas congelans partial 16S rRNA gene, type strain DSM 14939T
  • accession: AJ492828
  • length: 1531
  • database: ena
  • NCBI tax ID: 200452

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas congelans DSM 14939GCA_900103225scaffoldncbi200452
66792Pseudomonas congelans strain DSM 14939200452.18wgspatric200452
66792Pseudomonas congelans LMG 214662663762773draftimg200452

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes90.962no
flagellatedyes72.105no
gram-positiveno98.248no
anaerobicno98.284yes
aerobicyes93.481yes
halophileno91.03no
spore-formingno94.575no
glucose-fermentno90.318yes
thermophileno99.761yes
glucose-utilyes95.66no

External links

@ref: 5612

culture collection no.: DSM 14939, LMG 21466, CCUG 51513, CFBP 7019, CIP 108180

straininfo link

  • @ref: 82295
  • straininfo: 86585

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny13130034Fluorescent pseudomonads associated with the phyllosphere of grasses; Pseudomonas trivialis sp. nov., Pseudomonas poae sp. nov. and Pseudomonas congelans sp. nov.Behrendt U, Ulrich A, Schumann PInt J Syst Evol Microbiol10.1099/ijs.0.02567-02003DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fluorescence, Molecular Sequence Data, Phenotype, Phylogeny, Poaceae/*microbiology, Pseudomonas/classification/genetics/*isolation & purification/metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species SpecificityGenetics
Phylogeny33999789Pseudomonas quercus sp. nov, associated with leaf spot disease of Quercus mongolica.Li Y, Wang S, Bian D, Sun S, Ma TInt J Syst Evol Microbiol10.1099/ijsem.0.0048002021Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Plant Diseases/*microbiology, Pseudomonas/*classification/isolation & purification, Quercus/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitle
5612Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14939)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14939
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39752Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5774
59223Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 51513)https://www.ccug.se/strain?id=51513
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82295Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID86585.1StrainInfo: A central database for resolving microbial strain identifiers
118729Curators of the CIPCollection of Institut Pasteur (CIP 108180)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108180