Strain identifier

BacDive ID: 13083

Type strain: Yes

Species: Pseudomonas poae

Strain Designation: P527/13

Strain history: CIP <- 2003, U. Behrendt, Paulinenaue, Germany: strain P527/13

NCBI tax ID(s): 200451 (species)

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General

@ref: 5609

BacDive-ID: 13083

DSM-Number: 14936

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Pseudomonas poae P527/13 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from phyllosphere of grasses.

NCBI tax id

  • NCBI tax id: 200451
  • Matching level: species

strain history

@refhistory
5609<- U. Behrendt; P527/13
367012003, U. Behrendt, Paulinenaue, Germany: strain P527/13
121917CIP <- 2003, U. Behrendt, Paulinenaue, Germany: strain P527/13

doi: 10.13145/bacdive13083.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas poae
  • full scientific name: Pseudomonas poae Behrendt et al. 2003

@ref: 5609

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas poae

full scientific name: Pseudomonas poae Behrendt et al. 2003

strain designation: P527/13

type strain: yes

Morphology

cell morphology

  • @ref: 121917
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 121917
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5609REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a)yeshttps://mediadive.dsmz.de/medium/220aName: REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a) Composition: Casein peptone 17.0 g/l Agar 15.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water
5609REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)yeshttps://mediadive.dsmz.de/medium/1aName: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
36701MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121917CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5609positivegrowth28mesophilic
36701positivegrowth30mesophilic
59222positivegrowth21-28
121917positivegrowth5-30
121917nogrowth37mesophilic
121917nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
59222aerobe
121917obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
121917NaClpositivegrowth0-6 %
121917NaClnogrowth8 %
121917NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
12191716947citrate-carbon source
1219174853esculin+hydrolysis
12191717632nitrate-reduction
12191716301nitrite-reduction
12191717632nitrate-respiration

antibiotic resistance

  • @ref: 121917
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12191735581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
121917oxidase+
121917beta-galactosidase-3.2.1.23
121917alcohol dehydrogenase-1.1.1.1
121917gelatinase-
121917amylase-
121917DNase-
121917caseinase-3.4.21.50
121917catalase+1.11.1.6
121917tween esterase+
121917lecithinase-
121917lipase-
121917lysine decarboxylase-4.1.1.18
121917ornithine decarboxylase-4.1.1.17
121917protease-
121917tryptophan deaminase-
121917urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121917-+++-+--+-++----+---

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
5609-----+--+++++-++-++-+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121917+++++--+----------++++----+----+++-----+---++--+++++-++++--++-+++------+++-+++-+++++--+-+++++++++++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5609phyllosphere of grassesBrandenburg, PaulinenaueGermanyDEUEurope
59222Phyllosphere of grassesGermanyDEUEurope
121917Phyllosphere of grassesGermanyDEUEurope1994

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Leaf (Phyllosphere)

taxonmaps

  • @ref: 69479
  • File name: preview.99_168.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_98;99_168&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: AJ492829
  • Sequence Identity:
  • Total samples: 5463
  • soil counts: 555
  • aquatic counts: 976
  • animal counts: 2173
  • plant counts: 1759

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
56091Risk group (German classification)
1219171Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5609
  • description: Pseudomonas poae partial 16S rRNA gene, type strain DSM 14936T
  • accession: AJ492829
  • length: 1529
  • database: ena
  • NCBI tax ID: 200451

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas poae DSM 14936GCA_001439785contigncbi200451
66792Pseudomonas poae LMG 21465GCA_001730605contigncbi200451
66792Pseudomonas poae strain DSM 14936200451.7wgspatric200451
66792Pseudomonas poae strain LMG 21465200451.9wgspatric200451
66792Pseudomonas poae LMG 214652917137258draftimg200451
66792Pseudomonas poae DSM 149362667527928draftimg200451
66792Pseudomonas poae LMG 214652639762637draftimg200451
66792Pseudomonas poae LMG 21465GCA_900103965chromosomencbi200451

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedyes76.255no
gram-positiveno98.294no
anaerobicno98.101no
aerobicyes93.168yes
halophileno87.504no
spore-formingno95.311no
glucose-fermentno91.489yes
thermophileno99.741yes
glucose-utilyes95.246yes
motileyes90.031no

External links

@ref: 5609

culture collection no.: DSM 14936, LMG 21465, CCUG 51512, CCM 7506, CIP 108181

straininfo link

  • @ref: 82293
  • straininfo: 86584

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny13130034Fluorescent pseudomonads associated with the phyllosphere of grasses; Pseudomonas trivialis sp. nov., Pseudomonas poae sp. nov. and Pseudomonas congelans sp. nov.Behrendt U, Ulrich A, Schumann PInt J Syst Evol Microbiol10.1099/ijs.0.02567-02003DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fluorescence, Molecular Sequence Data, Phenotype, Phylogeny, Poaceae/*microbiology, Pseudomonas/classification/genetics/*isolation & purification/metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species SpecificityGenetics
Phylogeny32228807Pseudomonas kitaguniensis sp. nov., a pathogen causing bacterial rot of Welsh onion in Japan.Sawada H, Fujikawa T, Nishiwaki Y, Horita HInt J Syst Evol Microbiol10.1099/ijsem.0.0041232020Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Japan, Multilocus Sequence Typing, Nucleic Acid Hybridization, Onions/*microbiology, *Phylogeny, Plant Diseases/*microbiology, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome
Phylogeny33270007Pseudomonas allii sp. nov., a pathogen causing soft rot of onion in Japan.Sawada H, Fujikawa T, Tsuji M, Satou MInt J Syst Evol Microbiol10.1099/ijsem.0.0045822020Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Japan, Nucleic Acid Hybridization, Onions/*microbiology, *Phylogeny, Plant Diseases/*microbiology, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome
Phylogeny33650945Pseudomonas cyclaminis sp. nov., a pathogen causing bacterial bud blight of cyclamen in Japan.Sawada H, Fujikawa T, Osada S, Satou MInt J Syst Evol Microbiol10.1099/ijsem.0.0047232021

Reference

@idauthorscataloguedoi/urltitle
5609Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14936)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14936
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
36701Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5775
59222Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 51512)https://www.ccug.se/strain?id=51512
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82293Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID86584.1StrainInfo: A central database for resolving microbial strain identifiers
121917Curators of the CIPCollection of Institut Pasteur (CIP 108181)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108181