Strain identifier

BacDive ID: 130688

Type strain: Yes

Species: Pleomorphochaeta multiformis

Strain Designation: MO-SPC2

Strain history: M. Miyazaki MO-SPC2.

NCBI tax ID(s): 913101 (species)

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General

@ref: 22086

BacDive-ID: 130688

DSM-Number: 23952

keywords: 16S sequence, Bacteria, anaerobe, chemoheterotroph, psychrophilic, Gram-negative

description: Pleomorphochaeta multiformis MO-SPC2 is an anaerobe, chemoheterotroph, psychrophilic bacterium that was isolated from subseafloor sediment, 1180 m water depth.

NCBI tax id

  • NCBI tax id: 913101
  • Matching level: species

strain history

@refhistory
22086<- M. Miyazaki, Japan Agency for Marine-Earth Sci. and Technol. (JAMSTEC), Yokosuka; MO-SPC2 <- H. Imachi {2010}
67770M. Miyazaki MO-SPC2.

doi: 10.13145/bacdive130688.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/spirochaetota
  • domain: Bacteria
  • phylum: Spirochaetota
  • class: Spirochaetia
  • order: Spirochaetales
  • family: Sphaerochaetaceae
  • genus: Pleomorphochaeta
  • species: Pleomorphochaeta multiformis
  • full scientific name: Pleomorphochaeta multiformis (Miyazaki et al. 2014) Arroua et al. 2017

  • synonyms

    • @ref: 20215
    • synonym: Sphaerochaeta multiformis

@ref: 22086

domain: Bacteria

phylum: Spirochaetes

class: Spirochaetia

order: Spirochaetales

family: Sphaerochaetaceae

genus: Pleomorphochaeta

species: Pleomorphochaeta multiformis

full scientific name: Pleomorphochaeta multiformis (Miyazaki et al. 2014) Arroua et al. 2017

strain designation: MO-SPC2

type strain: yes

Morphology

cell morphology

  • @ref: 25202
  • gram stain: negative

colony morphology

  • @ref: 22086
  • incubation period: 8-14 days

Culture and growth conditions

culture medium

  • @ref: 22086
  • name: PLEOMORPHOCHAETA MEDIUM (DSMZ Medium 1271)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1271
  • composition: Name: PLEOMORPHOCHAETA MEDIUM (DSMZ Medium 1271; with strain-specific modifications) Composition: NaCl 24.9252 g/l MgCl2 x 6 H2O 3.98804 g/l Maltose 1.99402 g/l Na2CO3 1.49551 g/l CaCl2 x 2 H2O 0.997009 g/l Yeast extract 0.997009 g/l NH4Cl 0.498504 g/l Na2S x 9 H2O 0.299103 g/l L-Cysteine HCl x H2O 0.299103 g/l KH2PO4 0.0997009 g/l FeCl2 x 4 H2O 0.00199402 g/l NaOH 0.000498504 g/l Na2-EDTA 0.000498504 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l AlCl3 9.97009e-06 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
22086positivegrowth10psychrophilic
67770positivegrowth15psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
22086anaerobe
25202anaerobe

nutrition type

  • @ref: 25202
  • type: chemoheterotroph

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
22086--+--++--+------+----+/--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
22086subseafloor sediment, 1180 m water depthnorth-western Pacific Ocean off the Shimokita Peninsula (site C9001)JapanJPNAsia41.1773142.201
25202marine subsurface sediments
67770Marine subsurface sediments

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_184503.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_7468;96_51325;97_66108;98_100758;99_184503&stattab=map
  • Last taxonomy: Pleomorphochaeta multiformis subclade
  • 16S sequence: AB598280
  • Sequence Identity:
  • Total samples: 1350
  • soil counts: 30
  • aquatic counts: 1204
  • animal counts: 106
  • plant counts: 10

Safety information

risk assessment

  • @ref: 22086
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22086
  • description: Sphaerochaeta multiformis gene for 16S rRNA, partial sequence
  • accession: AB598280
  • length: 1466
  • database: ena
  • NCBI tax ID: 913101

GC content

  • @ref: 22086
  • GC-content: 32.3
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 22086

culture collection no.: DSM 23952, JCM 17281

straininfo link

  • @ref: 89195
  • straininfo: 402312

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny25249566Sphaerochaeta multiformis sp. nov., an anaerobic, psychrophilic bacterium isolated from subseafloor sediment, and emended description of the genus Sphaerochaeta.Miyazaki M, Sakai S, Ritalahti KM, Saito Y, Yamanaka Y, Saito Y, Tame A, Uematsu K, Loffler FE, Takai K, Imachi HInt J Syst Evol Microbiol10.1099/ijs.0.068148-02014Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Japan, Molecular Sequence Data, Pacific Ocean, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Sequence Analysis, DNA, Spirochaetaceae/*classification/genetics/isolation & purificationGenetics
Phylogeny27902274Pleomorphochaeta caudata gen. nov., sp. nov., an anaerobic bacterium isolated from an offshore oil well, reclassification of Sphaerochaeta multiformis MO-SPC2T as Pleomorphochaeta multiformis MO-SPC2T comb. nov. as the type strain of this novel genus and emended description of the genus Sphaerochaeta.Arroua B, Ranchou-Peyruse A, Ranchou-Peyruse M, Magot M, Urios L, Grimaud RInt J Syst Evol Microbiol10.1099/ijsem.0.0016412017Bacterial Typing Techniques, Base Composition, Congo, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Oil and Gas Fields/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Spirochaetaceae/*classification/genetics/isolation & purificationTranscriptome
Phylogeny30307390Pleomorphochaeta naphthae sp. nov., a new anaerobic fermentative bacterium isolated from an oil field.Arroua B, Grimaud R, Hirschler-Rea A, Bouriat P, Magot M, Urios L, Ranchou-Peyruse AInt J Syst Evol Microbiol10.1099/ijsem.0.0030482018Bacterial Typing Techniques, Base Composition, Congo, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Oil and Gas Fields/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spirochaetaceae/*classification/genetics/isolation & purificationTranscriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22086Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-23952Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23952)
25202B. R.-P. Arroua, A.,Ranchou-Peyruse, M.,Magot, M.,Urios, L.,Grimaud, R.Pleomorphochaeta caudata gen. nov., sp. nov., an anaerobic bacterium isolated from an offshore oil well, reclassification of Sphaerochaeta multiformis MO-SPC2T as Pleomorphochaeta multiformis MO-SPC2T comb. nov. as the type strain of this novel genus and emended description of the genus Sphaerochaeta10.1099/ijsem.0.001641IJSEM 67: 417-424 201727902274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
89195Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID402312.1