Strain identifier
BacDive ID: 130666
Type strain: 
Species: Novosphingobium chloroacetimidivorans
Strain Designation: BUT-14
Strain history: Q. Chen BUT-14.
NCBI tax ID(s): 1428314 (species)
version 8.1 (current version)
General
@ref: 21702
BacDive-ID: 130666
DSM-Number: 100345
keywords: 16S sequence, Bacteria, mesophilic
description: Novosphingobium chloroacetimidivorans BUT-14 is a mesophilic bacterium that was isolated from activated sludge of wastewater treatment plant of a chloroacetamide-herbicides-manufacturing facility.
NCBI tax id
- NCBI tax id: 1428314
- Matching level: species
strain history
| @ref | history |
|---|---|
| 21702 | <- KACC <- S.-W. Kwon, KACC <- Q. Chen, Nanjing Agricultural University, China |
| 67770 | Q. Chen BUT-14. |
doi: 10.13145/bacdive130666.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Sphingomonadales
- family: Erythrobacteraceae
- genus: Novosphingobium
- species: Novosphingobium chloroacetimidivorans
- full scientific name: Novosphingobium chloroacetimidivorans Chen et al. 2014
@ref: 21702
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Pseudomonadales
family: Erythrobacteraceae
genus: Novosphingobium
species: Novosphingobium chloroacetimidivorans
full scientific name: Novosphingobium chloroacetimidivorans Chen et al. 2014
strain designation: BUT-14
type strain: yes
Culture and growth conditions
culture medium
- @ref: 21702
- name: R2A MEDIUM (DSMZ Medium 830)
- growth: yes
- link: https://mediadive.dsmz.de/medium/830
- composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 21702 | positive | growth | 28 | mesophilic |
| 67770 | positive | growth | 30 | mesophilic |
Physiology and metabolism
observation
- @ref: 67770
- observation: quinones: Q-10
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
|---|---|---|---|---|---|---|---|
| 21702 | activated sludge of wastewater treatment plant of a chloroacetamide-herbicides-manufacturing facility | Kunshan City, Jinagsu province | China | CHN | Asia | 31.3667 | 120.933 |
| 67770 | Activated sludge of a chloroacetamide-herbicides-manufacturing wastewater treatment facility |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Industrial | |
| #Engineered | #Waste | #Activated sludge |
| #Engineered | #Waste | #Industrial wastewater |
| #Engineered | #Waste | #Water treatment plant |
Safety information
risk assessment
- @ref: 21702
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21702
- description: Novosphingobium chloroacetimidivorans strain BUT-14 16S ribosomal RNA gene, partial sequence
- accession: KF676669
- length: 1445
- database: ena
- NCBI tax ID: 1428314
GC content
- @ref: 67770
- GC-content: 66
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 21702
culture collection no.: DSM 100345, CCTCC AB 2013086, JCM 19923, KACC 17147, BCRC 80813
straininfo link
- @ref: 89174
- straininfo: 400560
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 24814333 | Novosphingobium chloroacetimidivorans sp. nov., a chloroacetamide herbicide-degrading bacterium isolated from activated sludge. | Chen Q, Zhang J, Wang CH, Jiang J, Kwon SW, Sun LN, Shen WB, He J | Int J Syst Evol Microbiol | 10.1099/ijs.0.062950-0 | 2014 | Acetamides, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Herbicides, Japan, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Spermidine/chemistry, Sphingomonadaceae/*classification/genetics/isolation & purification, Ubiquinone/chemistry, Waste Disposal Facilities | Genetics |
| Phylogeny | 25667393 | Novosphingobium fluoreni sp. nov., isolated from rice seeds. | Gao S, Zhang Y, Jiang N, Luo L, Li QX, Li J | Int J Syst Evol Microbiol | 10.1099/ijs.0.000111 | 2015 | Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Oryza/*microbiology, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Seeds/*microbiology, Sequence Analysis, DNA, Spermidine/chemistry, Sphingomonadaceae/*classification/genetics/isolation & purification, Ubiquinone/chemistry | Genetics |
| Phylogeny | 28866996 | Novosphingobium ipomoeae sp. nov., isolated from a water convolvulus field. | Chen WM, Huang CW, Chen JC, Chen ZH, Sheu SY | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002174 | 2017 | Bacterial Typing Techniques, Base Composition, Convolvulus/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spermidine/chemistry, Sphingomonadaceae/*classification/genetics/isolation & purification, Taiwan, Ubiquinone/chemistry | Transcriptome |
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 21702 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-100345 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100345) | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 89174 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID400560.1 |